水貂TYR基因T138A位点多态性及其与毛色性状的关联分析

doi:10.16431/j.cnki.1671-7236.2017.10.020

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (10): 2973-2979.doi: 10.16431/j.cnki.1671-7236.2017.10.020

水貂TYR基因T138A位点多态性及其与毛色性状的关联分析

宋兴超, 徐超, 刘宗岳, 杨童奥, 岳志刚, 刘琳玲, 丛波, 杨福合

中国农业科学院特产研究所, 吉林省特种经济动物分子生物学省部共建国家重点实验室, 长春 130112

收稿日期:2017-04-10 出版日期:2017-10-20 发布日期:2017-10-20
通讯作者: 杨福合 E-mail:yangfh@126.com
作者简介:宋兴超(1982-),男,河北保定人,博士,助理研究员,研究方向:特种经济动物遗传育种,E-mail:songxingchao@caas.cn
基金资助:
国家重点基础研究发展计划-973项目（2012CB722907）；水貂优良种质资源及选育技术引进-948项目（2014-Z8）；中国农业科学院科技创新工程—特种动物育种科技创新团队（CAAS-ASTIP-2016-ISAPS02）；中央级公益性科研院所基本科研业务费专项（1610342017011）

Relationship Analysis Between the Polymorphism of T138A Locus in TYR Gene and Hair Color in Mink (Neovison vison)

SONG Xing-chao, XU Chao, LIU Zong-yue, YANG Tong-ao, YUE Zhi-gang, LIU Lin-ling, CONG Bo, YANG Fu-he

State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received:2017-04-10 Online:2017-10-20 Published:2017-10-20

摘要/Abstract

摘要：

试验旨在检测水貂酪氨酸酶（tyrosinase，TYR）基因T138A位点的多态性，并分析其与水貂毛色表型的相关性。提取5种被毛色型430只水貂血液基因组DNA，采用PCR-RFLP技术，对TYR基因T138A位点进行多态性检测，统计等位基因频率与基因型频率，通过卡方（χ²）独立性检验分析该位点多态性与水貂毛色性状的相关性。结果表明，T138A位点存在2个等位基因T和A，形成TT、TA和AA 3种基因型，AA基因型在吉林白水貂群体中为优势基因型（0.9069），而TT基因型为金州黑水貂、珍珠水貂、咖啡水貂和银蓝水貂群体的优势基因型，其中在金州黑水貂群体中基因型频率最高（1.0000）。关联分析表明，TYR基因T138A位点的多态性与毛色性状呈极显著相关（P<0.0001）。表明TYR基因T138A位点可能是影响水貂毛色的主控位点或与调控白色被毛表型主控位点连锁的分子标记。

关键词: 水貂; TYR基因; T138A位点; PCR-RFLP; 毛色

Abstract:

In order to detect the polymorphism of T138A locus in tyrosinase (TYR) gene in mink and analyze the relationship between the genetic polymorphisms and phenotypes of mink hair color, the blood samples of 430 minks with five kinds of different hair color were taken and their genomic DNA were extracted. The T138A locus of TYR gene in mink was detected using PCR-RFLP method. Allele frequency and genotype frequency were calculated. Furthermore, the relationship between the polymorphism of T138A locus and hair color trait were analyzed by the statistical method of Chi-square independence test. The results showed that the T138A locus polymorphism was found with two alleles T and A,and three genotypes of TT, TA and AA. AA genotype was dominant genotype in Jilin White mink (0.9069), TT genotype was dominant genotype in Jinzhou Black mink,Pearl mink,Coffee mink and Silverblue mink, and existed mainly in Jizhou Black mink (1.0000). The association analysis of T138A locus polymorphism with hair color trait indicated that there was extremely significant correlation between TYR gene polymorphism and hair color of mink (P < 0.0001). This results indicated that the T138A locus might affect hair color phenotype, or molecular marker linked with the major gene regulating the white hair phenotype of mink.

Key words: mink; TYR gene; T138A locus; PCR-RFLP; hair color

中图分类号:

S813.3

宋兴超, 徐超, 刘宗岳, 杨童奥, 岳志刚, 刘琳玲, 丛波, 杨福合. 水貂TYR基因T138A位点多态性及其与毛色性状的关联分析[J]. 《中国畜牧兽医》, 2017, 44(10): 2973-2979.

SONG Xing-chao, XU Chao, LIU Zong-yue, YANG Tong-ao, YUE Zhi-gang, LIU Lin-ling, CONG Bo, YANG Fu-he. Relationship Analysis Between the Polymorphism of T138A Locus in TYR Gene and Hair Color in Mink (Neovison vison)[J]. , 2017, 44(10): 2973-2979.

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水貂TYR基因T138A位点多态性及其与毛色性状的关联分析

Relationship Analysis Between the Polymorphism of T138A Locus in TYR Gene and Hair Color in Mink (Neovison vison)

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