陆川猪MC1R和KIT基因型鉴定

doi:10.16431/j.cnki.1671-7236.2017.03.022

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (3): 773-781.doi: 10.16431/j.cnki.1671-7236.2017.03.022

陆川猪MC1R和KIT基因型鉴定

秦珂¹, 黄翔¹, 曾检华², 王晨¹, 孙冠杰¹, 宋德清², 陈瑶生¹, 刘小红¹, 何祖勇¹

1. 中山大学生命科学学院, 有害生物控制与资源利用国家重点实验室, 广州 510006;
2. 广东壹号食品股份有限公司, 广州 510620

收稿日期:2016-09-01 出版日期:2017-03-20 发布日期:2017-03-21
通讯作者: 何祖勇 E-mail:zuyonghe@foxmail.com
作者简介:秦珂(1989-),男,安徽亳州人,硕士,研究方向:动物学,E-mail:949612306@qq.com;黄翔(1981-),男,江西上饶人,博士,研究方向:动物遗传与育种,E-mail:huangxiangxms@163.com
基金资助:
科技基础性工作专项（2014FY120800）；广东省省级科技计划（2014B020202001）；广东省扬帆计划（2014YT02H042）；广东省自然科学基金（2016A030313310）；国家转基因生物新品种培育重大专项（2016ZX08006003-006）

Characterization of MC1R and KIT Genotypes in Luchuan Pig Population

QIN Ke¹, HUANG Xiang¹, ZENG Jian-hua², WANG Chen¹, SUN Guan-jie¹, SONG De-qing², CHEN Yao-sheng¹, LIU Xiao-hong¹, HE Zu-yong¹

1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China;
2. Guangdong YIHAO Food Co., Ltd., Guangzhou 510620, China

Received:2016-09-01 Online:2017-03-20 Published:2017-03-21

摘要/Abstract

摘要：

为了检测陆川猪种群的纯度，本研究以4个华南地方猪种（莆田黑猪、粤东黑猪、大花白猪、巴马香猪）和3个外来猪种（杜洛克猪、大白猪、长白猪）作为对照，利用PCR测序法对陆川猪群体56个样本的MC1R和KIT基因进行基因型鉴定。测序结果表明，大白猪和长白猪KIT基因内含子17的第1个碱基发生G>A剪接突变，而陆川猪和4个华南地方猪种，以及杜洛克猪中KIT基因型一样，均为野生型的GG基因型；MC1R基因编码区分析表明，以海南野猪MC1R基因型作为参考序列，陆川猪和4个华南地方猪种在MC1R基因编码区存在95Val > Met和102Leu > Pro两个错义突变；MC1R基因非编码区分析表明，与大白猪、长白猪和杜洛克猪相比，陆川猪和4个华南地方猪种MC1R基因的5'UTR分别存在5~6个SNPs，3'UTR存在1个A碱基的缺失。另外，试验对一窝毛色异常的陆川猪仔猪及其亲本的KIT和MC1R基因进行测序分析，结果表明，仔猪及其亲本的KIT基因型均为GG野生型，仔猪和母本的MC1R基因均存在E^D1和E^p两种不同的基因型，父本的MC1R基因型为E^D1，因E^p对应于皮特兰猪的斑块表型，所以初步判定母本渗入了外来猪种的血统。本研究通过检测陆川猪等8个猪种的毛色相关基因KIT和MC1R基因的基因型，证实了中外猪种间在毛色遗传上的分子差异，为今后深入研究猪毛色遗传的分子调控机理提供参考。

关键词: 陆川猪; 毛色; KIT基因; MC1R基因

Abstract:

In order to assess the purity of Luchuan pig populations, four South Chinese local pig breeds including Putian Black pig, Yuedong Black pig, Dahuabai pig, Bama miniature pig and three foreign pig breeds including Duroc pig, Yorkshire pig and Landrace pig were studied as controls by sequencing of PCR products, MC1R and KIT genotypes in 56 Luchuan pigs were analyzed in this study. Sequencing results indicated that a splicing mutation (G>A) was presented in the first base in intron 17 of KIT gene in both Yorkshire pig and Landrace pig, in contrast, the wildtype GG of KIT gene was presented in Luchuan pig, four south Chinese local pig breeds and Duroc pig.Compared with Hainan wild boar, South Chinese local pig breeds had two missense mutations 95Val > Met and 102Leu > Pro in the coding region of MC1R gene;Compared with Yorkshire pig, Landrace pig and Duroc pig, South Chinese local pig breeds had 5 to 6 SNPs in MC1R gene 5'UTR, and in addtion, an A base deletion in MC1R gene 3'UTR. Furthermore, we found one litter of Luchuan pig with abnormal coat color.The results showed that the presentation of two distinct MC1R genotypes E^D1 and E^p in both litters and the sow,but only E^D1 in the boar. Considering E^p was derived from Pietrain pig, we preliminarily considered that the genome of the sow might be infiltrated with foreign pig breeds. In summary, we detected the genotypes of the coat color genes KIT and MC1R in eight pig breeds, confirmed the molecular differences of coat color between Chinese local pig breeds and foreign pig breeds, which could be useful for the further investigation of the molecular mechanism of pig coat color.

Key words: Luchuan pig; coat color; KIT gene; MC1R gene

中图分类号:

S813.3

秦珂, 黄翔, 曾检华, 王晨, 孙冠杰, 宋德清, 陈瑶生, 刘小红, 何祖勇. 陆川猪MC1R和KIT基因型鉴定[J]. 《中国畜牧兽医》, 2017, 44(3): 773-781.

QIN Ke, HUANG Xiang, ZENG Jian-hua, WANG Chen, SUN Guan-jie, SONG De-qing, CHEN Yao-sheng, LIU Xiao-hong, HE Zu-yong. Characterization of MC1R and KIT Genotypes in Luchuan Pig Population[J]. , 2017, 44(3): 773-781.

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陆川猪MC1R和KIT基因型鉴定

Characterization of MC1R and KIT Genotypes in Luchuan Pig Population

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