陆川猪PTTG1基因克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2016.09.002

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (9): 2223-2229.doi: 10.16431/j.cnki.1671-7236.2016.09.002

陆川猪PTTG1基因克隆及序列分析

覃兆鲜, 农素群, 关志惠, 梁旦, 覃倩, 蓝晞, 潘天彪, 谢炳坤

广西壮族自治区畜牧研究所, 广西家畜遗传改良重点实验室, 南宁 530001

收稿日期:2016-03-07 出版日期:2016-09-20 发布日期:2016-09-20
通讯作者: 谢炳坤 E-mail:bkxie@163.com
作者简介:覃兆鲜(1983-),女,广西象州人,硕士,助理研究员,研究方向:动物基因工程,E-mail:zhx_qin@163.com
基金资助:
国家自然科学基金（31360544）；广西自然科学基金（2013GXNSFBA019117）；广西科学研究与技术开发计划项目（桂科重14121003-2-2）；广西水产畜牧科技推广应用项目（桂渔牧科201452020）

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig

QIN Zhao-xian, NONG Su-qun, GUAN Zhi-hui, LIANG Dan, QIN Qian, LAN Xi, PAN Tian-biao, XIE Bing-kun

Guangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Institute of Animal Sciences, Nanning 530001, China

Received:2016-03-07 Online:2016-09-20 Published:2016-09-20

摘要/Abstract

摘要：

试验旨在克隆陆川猪PTTG1基因全长CDS序列并对其进行生物信息学分析。利用GenBank公布的猪PTTG1预测序列设计引物，用RT-PCR扩增得到目的基因片段，并用生物信息学软件分析和预测了陆川猪PTTG1基因的理化性质与二级结构。结果表明，陆川猪PTTG1基因全长CDS序列为609 bp，编码202个氨基酸；其核苷酸序列与牛、黑猩猩、人、猕猴、大鼠、小鼠、原鸡和斑马鱼相对应序列同源性分别为90.15%、87.85%、87.52%、87.03%、76.03%、74.38%、55.74%和44.48%；PTTG1基因编码的蛋白无信号肽，属于亲水性蛋白，主要存在于细胞质中，存在16个磷酸化位点。氨基酸系统进化树分析表明，不同物种PTTG1基因在进化过程中具有高度保守性。本研究成功克隆了陆川猪PTTG1基因，为今后研究PTTG1基因在猪早期胚胎发育过程中的作用奠定理论基础。

关键词: 陆川猪; PTTG1基因; 克隆; 序列分析

Abstract:

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.

Key words: Luchuan pig; PTTG1 gene; cloning; sequence analysis

中图分类号:

Q785

覃兆鲜, 农素群, 关志惠, 梁旦, 覃倩, 蓝晞, 潘天彪, 谢炳坤. 陆川猪PTTG1基因克隆及序列分析[J]. 《中国畜牧兽医》, 2016, 43(9): 2223-2229.

QIN Zhao-xian, NONG Su-qun, GUAN Zhi-hui, LIANG Dan, QIN Qian, LAN Xi, PAN Tian-biao, XIE Bing-kun. Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig[J]. , 2016, 43(9): 2223-2229.

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陆川猪PTTG1基因克隆及序列分析

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig

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