猪源携带质粒介导喹诺酮类耐药基因大肠杆菌气源性传播的研究

doi:10.16431/j.cnki.1671-7236.2015.07.032

›› 2015, Vol. 42 ›› Issue (7): 1842-1850.doi: 10.16431/j.cnki.1671-7236.2015.07.032

猪源携带质粒介导喹诺酮类耐药基因大肠杆菌气源性传播的研究

张晓丹, 高丽丽, 胡家卿, 艾文豪, 柴同杰

山东农业大学动物科技学院, 泰安 271000

收稿日期:2014-11-27 出版日期:2015-07-20 发布日期:2015-07-23
通讯作者: 柴同杰 E-mail:chaitj117@163.com
作者简介:张晓丹(1989-),女,山东威海人,硕士生,研究方向:环境微生物,E-mail:zhangxiaodan89@126.com
基金资助:
国家自然科学基金(31270172)

Study on Aerotransmission of Escherichia coli Carrying the Plasmid-mediated Quinolone Resistance Genes of Swine

ZHANG Xiao-dan, GAO Li-li, HU Jia-qing, AI Wen-hao, CHAI Tong-jie

College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, China

Received:2014-11-27 Online:2015-07-20 Published:2015-07-23

摘要/Abstract

摘要： 为了调查养猪场携带质粒介导喹诺酮类耐药基因大肠杆菌的气源性传播情况,本试验分别在5个猪场舍内及舍外上风向和下风向不同距离收集空气样品,并在舍内随机采集粪便样品,分离大肠杆菌。药敏试验检测其对14种抗生素的耐药性。以质粒介导喹诺酮类耐药基因(qnr、aac(6')-Ib-cr、qepA)为指示基因,利用肠杆菌基因间重复一致序列聚合酶链式反应(ERIC-PCR)鉴定技术,分别对5个猪场不同样品中大肠杆菌的遗传相似性进行分析,评估其向舍外空气的传播情况。结果显示,5个猪场中的大肠杆菌对庆大霉素、卡那霉素、四环素、链霉素、萘啶酸、复方新诺明等12种常用抗生素耐药率较高,且呈现多重耐药。ERIC-PCR结果显示,46.34% (19/41)的舍内空气分离株与粪便分离株来源相同,其中73.68% (14/19)的分离株携带的耐药基因也相同;68.42% (26/38)的舍外空气分离株与舍内空气或粪便分离株来源相同,其中65.38% (17/26)的菌株携带相同的耐药基因。结果表明,起源于舍内粪便的携带质粒介导喹诺酮类耐药基因的大肠杆菌能形成气溶胶污染舍内空气,并借助舍内外气体交换,传播到舍外不同距离空气中(≥400 m),对养殖场周围的环境卫生及社区居民的健康形成威胁。

关键词: 大肠杆菌气溶胶; 耐药现状; 质粒介导喹诺酮类耐药(PMQR)基因; 遗传相似性; 公共卫生

Abstract: To investigate the aerotransmission of Escherichia coli (E.coli) carrying the plasmid-mediated quinolone resistance (PMQR) genes,airborne E.coli were isolated from indoor air,upwind air and downwind air samples in five swine farms.Fecal samples from swine houses were randomly collected to isolate the E.coli.The sensitivities of the E.coli strains against 14 antibiotics were tested.The E.coli carrying the PMQR genes (qnr,aac(6')-Ib-cr,qepA) were identified by ERIC-PCR,and then the genetic fingerprints of E.coli were established to analyze its origins and spread toward the outside surroundings.The results showed that E.coli isolated from five swine farms showed high resistance against 12 antibiotics,such as gentamicin,kanamycin,tetracycline,streptomycin,nalidixic acid and sulfamethoxazole,and presented multi-drug resistant.Results of ERIC-PCR showed that 46.34% (19/41) of strains isolated from indoor air samples had the same origin with fecal-obtained strains,and 73.68% (14/19) of them shared the same PMQR genes with fecal-obtained strains.68.42% (26/38) of strains isolated from downwind air samples had the same origin with fecal-obtained or indoor air-obtained strains,and 65.38% (17/26) of them shared the same PMQR genes with fecal-obtained or indoor air-obtained strains.This indicated that E.coli carrying PMQR genes and originating from feces in swine houses could form aerosols to pollute the indoor air and then spread to the downwind air through air exchange (≥400 m),which could be a potential threaten to public environment and human health.

Key words: E.coli aerosols; resistance status; plasmid-mediated quinolone resistance (PMQR) genes; genetic similarity; public health

中图分类号:

S852.61

张晓丹, 高丽丽, 胡家卿, 艾文豪, 柴同杰. 猪源携带质粒介导喹诺酮类耐药基因大肠杆菌气源性传播的研究[J]. , 2015, 42(7): 1842-1850.

ZHANG Xiao-dan, GAO Li-li, HU Jia-qing, AI Wen-hao, CHAI Tong-jie. Study on Aerotransmission of Escherichia coli Carrying the Plasmid-mediated Quinolone Resistance Genes of Swine[J]. , 2015, 42(7): 1842-1850.

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猪源携带质粒介导喹诺酮类耐药基因大肠杆菌气源性传播的研究

Study on Aerotransmission of Escherichia coli Carrying the Plasmid-mediated Quinolone Resistance Genes of Swine

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