H10N8亚型禽流感病毒的研究进展

摘要/Abstract

摘要： 中国于2013年底首次发现H10N8亚型禽流感病毒（AIV）能感染人并致死的病例，而在此之前从未有过关于H10N8亚型病毒能感染人的报道，目前也未在其他国家监测到H10N8亚型AIV感染人的病例。与H7N9亚型AIV相似，H10N8亚型AIV在家禽中致病性很弱，可一旦感染人能表现出很强的致病性。感染了H10N8亚型AIV的患者病情恶化快、死亡率高、对人的健康危害很大，但至今尚无有效的防制手段。目前还不太清楚H10N8亚型AIV是通过何种途径传播给人的，今后H10N8亚型AIV是否会在人群中引发新的疫情暴发是人们普遍关心的问题。文章简要介绍了H10N8亚型AIV的特点及研究现状。

关键词: 禽流感病毒; 基因重配; H10N8; H7N9; H5N1

Abstract: It was found for the first time at the end of 2013 in China that avian influenza A virus subtype H10N8 could infect human beings and result in death. No such a case has been reported in other parts of the world. Similarly to H7N9, H10N8 AIV is less pathogenic in poultry. However, when an H10N8 virus infects a human being, the virus can become highly pathogenic. A person infected with an H10N8 AIV deteriorated quickly and the mortality rate is high, and H10N8 infection poses a great threat to human health. At present there are no effective means to prevent and control H10N8 AIV infection. It is currently not clear how an H10N8 AIV is transmitted to humans. There are great concerns in public whether an H10N8 AIV will cause another outbreak in humans. This article will briefly describe the characteristics of H10N8 subtype viruses and the current research on these viruses.

Key words: avian influenza virus; gene reassortment; H10N8; H7N9; H5N1

谭伟，谢芝勋. H10N8亚型禽流感病毒的研究进展[J]. 中国畜牧兽医.

TAN Wei, XIE Zhi-xun. Research Progress on Avian Influenza A Virus Subtype H10N8[J]. .

参考文献

1 刘永宏，赵丽，代艳艳. 新型重配A（H7N9）流感病毒NA基因序列分析［J］. 中国畜牧兽医，2014，41(2)：43～49.
2 朱闻斐，高荣保，王大燕，等. H7亚型禽流感病毒概述［J］. 病毒学报，2013，29(3)：245～249.
3 陈化兰，于康震，卢景良. 禽流感病毒及其分子生物学研究进展［J］. 国外兽医学，1997，17(1)：3～7.
4 罗思思，谢芝勋，刘加波，等. H7N9亚型禽流感病毒三重PCR检测方法的建立［J］. 中国畜牧兽医，2014，41(2)：61～64.
5 徐晓龙，包红梅，陈化兰，等. 影响禽流感病毒致病性和传播的关键氨基酸位点的研究进展［J］. 中国预防兽医学报，2014，36(2)：165～168.
6 郭捷，谢芝勋，罗思思，等. 巢式PCR检测H1亚型禽流感病毒方法的建立［J］. 中国畜牧兽医，2013，40(9)：23～26.
7 谭伟，徐倩，谢芝勋. 禽流感病毒研究概述［J］. 基因组学与应用生物学，2014，33(1)：194～199.
8 Arzey G G, Kirkland P D, Arzey K E, et al. Influenza virus A (H10N7) in chickens and poultry abattoir workers, Australia［J］. Emerg Infect Dis, 2012, 18(5): 814～816.
9 Chan J F, To K K, Tse H, et al. Interspecies transmission and emergence of novel viruses: Lessons from bats and birds［J］. Trends Microbiol, 2013, 21(10): 544～555.
10 Chen H, Yuan H, Gao R, et al. Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection: A descriptive study［J］. Lancet, 2014, 383(9918): 714～721.
11 Cheng V C, Chan J F, Wen X, et al. Infection of immunocompromised patients by avian H9N2 influenza A virus［J］. J Infect, 2011, 62(5): 394～399.
12 Cheng V C, To K K, Tse H, et al. Two years after pandemic influenza A/2009/H1N1: What have we learned?［J］. Clin Microbiol Rev, 2012, 25(2): 223～263.
13 Cowling B J, Jin L, Lau E H, et al. Comparative epidemiology of human infections with avian influenza A H7N9 and H5N1 viruses in China: A population-based study of laboratory-confirmed cases［J］. Lancet, 2013, 382(9887): 129～137.
14 de Jong M D, Simmons C P, Thanh T T, et al. Fatal outcome of human influenza A (H5N1) is associated with high viral load and hypercytokinemia［J］. Nat Med, 2006, 12(10): 1203～1207.
15 De Marco M A, Campitelli L, Foni E, et al. Influenza surveillance in birds in Italian wetlands (1992—1998): Is there a host restricted circulation of influenza viruses in sympatric ducks and coots?［J］. Vet Microbiol, 2004, 98(3～4): 197～208.
16 de Wit E, Fouchier R A. Emerging influenza［J］. J Clin Virol, 2008, 41(1): 1～6.
17 de Wit E, Munster V J, van Riel D, et al. Molecular determinants of adaptation of highly pathogenic avian influenza H7N7 viruses to efficient replication in the human host［J］. J Virol, 2010, 84(3): 1597～1606.
18 El Zowalaty M E, Bustin S A, Husseiny M I, et al. Avian influenza: Virology, diagnosis and surveillance［J］. Future Microbiol, 2013, 8(9): 1209～1227.
19 Englund L. Studies on influenza viruses H10N4 and H10N7 of avian origin in mink［J］. Vet Microbiol, 2000, 74(1～2): 101～107.
20 Fan S, Deng G, Song J, et al. Two amino acid residues in the matrix protein M1 contribute to the virulence difference of H5N1 avian influenza viruses in mice［J］. Virology, 2009, 384(1): 28～32.
21 Fouchier R A, Schneeberger P M, Rozendaal F W, et al. Avian influenza A virus (H7N7) associated with human conjunctivitis and a fatal case of acute respiratory distress syndrome［J］. Proc Natl Acad Sci USA, 2004, 101(5): 1356～1361.
22 Gao R, Cao B, Hu Y, et al. Human infection with a novel avian-origin influenza A (H7N9) virus［J］. N Engl J Med, 2013, 368(20): 1888～1897.
23 García-Sastre A, Schmolke M. Avian influenza A H10N8——A virus on the verge?［J］. Lancet, 2014, 383(9918): 676～677.
24 Hatta M, Gao P, Halfmann P, et al. Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses［J］. Science, 2001, 293(5536): 1840～1842.
25 Jiao P, Tian G, Li Y, et al. A single-amino-acid substitution in the NS1 protein changes the pathogenicity of H5N1 avian influenza viruses in mice［J］. J Virol, 2008, 82(3): 1146～1154.
26 Jiao P, Cao L, Yuan R, et al. Complete genome sequence of an H10N8 avian influenza virus isolated from a live bird market in Southern China［J］. J Virol, 2012, 86(14): 7716.
27 Klingeborn B, Englund L, Rott R, et al. An avian influenza A virus killing a mammalian species——The mink［J］. Arch Virol, 1985, 86(3～4): 347～351.
28 Matrosovich M, Zhou N, Kawaoka Y, et al. The surface glycoproteins of H5 influenza viruses isolated from humans, chickens, and wild aquatic birds have distinguishable properties［J］. J Virol, 1999, 73(2): 1146～1155.
29 Pitzer V E, Olsen S J, Bergstrom C T, et al. Little evidence for genetic susceptibility to influenza A (H5N1) from family clustering data［J］.Emerg Infect Dis, 2007, 13(7): 1074～1076.
30 Reperant L A, Kuiken T, Osterhaus A D. Influenza viruses: From birds to humans［J］. Hum Vaccin Immunother, 2012, 8(1): 7～16.
31 Senne D A, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: Amino acid sequence at the HA cleavage site as a marker of pathogenicity potential［J］. Avian Dis, 1996, 40(2): 425～437.
32 Shinya K, Ebina M, Yamada S, et al. Avian flu: Influenza virus receptors in the human airway［J］. Nature, 2006, 440(7083): 435～436.
33 Shtyrya Y A, Mochalova L V, Bovin N V. Influenza virus neuraminidase: Structure and function［J］. Acta Naturae, 2009, 1(2): 26～32.
34 Su S, Qi W B, Chen J D, et al. Complete genome sequence of an avian-like H4N8 swine influenza virus discovered in southern China［J］. J Virol, 2012, 86(17): 9542.
35 Sun Y, Tan Y, Wei K, et al. Amino acid 316 of hemagglutinin and the neuraminidase stalk length influence virulence of H9N2 influenza virus in chickens and mice［J］. J Virol, 2013, 87(5): 2963～2968.
36 Taubenberger J K, Reid A H, Lourens R M, et al. Characterization of the 1918 influenza virus polymerase genes［J］. Nature, 2005, 437(7060): 889～893.
37 To K K, Chan J F, Chen H, et al. The emergence of influenza A H7N9 in human beings 16 years after influenza A H5N1: A tale of two cities［J］. Lancet Infect Dis, 2013, 13(9): 809～821.
38 To K K, Tsang A K, Chan J F, et al. Emergence in China of human disease due to avian influenza A (H10N8)——Cause for concern?［J］. J Infect, 2014, 68(3): 205～215.
39 van Riel D, Munster V J, de Wit E, et al. H5N1 virus attachment to lower respiratory tract［J］. Science, 2006, 312(5772): 399.
40 Wan J, Zhang J, Tao W, et al. A report of first fetal case of H10N8 avian influenza virus pneumonia in the world［J］.Zhonghua Wei Zhong Bing Ji Jiu Yi Xue， 2014, 26(2): 120～122.
41 Wang N, Zou W, Yang Y, et al. Complete genome sequence of an H10N5 avian influenza virus isolated from pigs in central China［J］. J Virol, 2012, 86(24): 13865～13866.
42 Webster R G, Geraci J, Petursson G, et al. Conjunctivitis in human beings caused by influenza A virus of seals［J］. N Engl J Med, 1981, 304(15): 911.
43 Yuan J, Zhang L, Kan X, et al. Origin and molecular characteristics of a novel 2013 avian influenza A (H6N1) virus causing human infection in Taiwan［J］. Clin Infect Dis, 2013, 57(9): 1367～1368.
44 Zhang H, Xu B, Chen Q, et al. Characterization of an H10N8 influenza virus isolated from Dongting lake wetland［J］. Virol J, 2011, 8: 42.
45 Zohari S, Metreveli G, Kiss I, et al. Full genome comparison and characterization of avian H10 viruses with different pathogenicity in mink (Mustela vison) reveals genetic and functional differences in the non-structural gene［J］. Virol J, 2010, 7: 145.

[1]	龙凤, 谢守玉, 崔鹏飞, 施开创, 韦显凯, 冯淑萍, 屈素洁, 陆文俊, 李剑锋, 尹彦文, 邓国华. 2016－2021年广西H9亚型禽流感病毒HA和NA基因遗传进化动态分析[J]. 中国畜牧兽医, 2023, 50(5): 1947-1958.
[2]	谢守玉, 王睿敏, 崔鹏飞, 施开创, 韦显凯, 冯淑萍, 龙凤, 屈素洁, 陆文俊, 黄金山, 黄凤梅, 温新瑞, 尹彦文, 邓国华. 2株海鸭源H3亚型禽流感病毒全基因组分子特征分析[J]. 中国畜牧兽医, 2023, 50(4): 1319-1328.
[3]	杨景, 张新宇, 梁志鹏, 程晴, 王聪颖, 池仕红, 袁生, 郭锦玥, 黄淑坚, 温峰. H9N2亚型禽流感病毒NP蛋白原核表达及多克隆抗体制备[J]. 中国畜牧兽医, 2022, 49(10): 3963-3971.
[4]	江宁, 尹航, 张岩威, 李紫馨, 池晓娟, 王松. H9N2亚型禽流感病毒与其他病原混合感染的研究进展[J]. 中国畜牧兽医, 2021, 48(9): 3447-3455.
[5]	尹彦文, 施开创, 孙文超, 谢守玉, 屈素洁, 陆文俊, 王露霞, 覃勇, 裴幸彪, 凌丹. 2013－2019年广西边境地区低致病性禽流感病毒监测分析[J]. 中国畜牧兽医, 2021, 48(8): 3002-3009.
[6]	李占鸿, 朱建波, 寇美玲, 杨振兴, 李卓然, 牛保生, 姚萍芬, 李华春, 杨恒. 1株库蠓源版纳病毒的分离与全基因组序列分析[J]. 中国畜牧兽医, 2021, 48(3): 781-791.
[7]	程邓芳, 梅晨, 刘娟, 王宏俊, 先宏. 中药复方对H9N2亚型禽流感病毒在MDCK细胞增殖的作用[J]. 中国畜牧兽医, 2021, 48(2): 650-657.
[8]	李青梅, 郭军庆, 孟泽锟, 李艳华, 刘肖, 石建州, 李鸽, 柴书军, 罗俊, 邓瑞广, 张改平. H9N2亚型禽流感病毒中和单克隆抗体的制备与应用[J]. 中国畜牧兽医, 2021, 48(10): 3761-3769.
[9]	程艺, 田辉, 张亚, 薛景景, 盛东北, 刘武杰, 王婉冰, 张盼涛, 田克恭. H9亚型禽流感病毒HF株的分离鉴定和免疫原性评价[J]. 中国畜牧兽医, 2020, 47(9): 2945-2952.
[10]	李佳佳, 李素华, 宋建领, 黄景军, 缪渊, 陈曦, 季佳. 2株云南H9N2亚型禽流感病毒HA和NA基因序列分析[J]. 中国畜牧兽医, 2020, 47(3): 873-883.
[11]	彭成成, 吴熠潇, 刘旭平, 谭文松. H9N2亚型禽流感病毒纯化及病毒HA蛋白定量方法研究[J]. 《中国畜牧兽医》, 2019, 46(7): 2069-2078.
[12]	熊文婕, 谢芝勋, 李孟, 曹国敏, 覃海, 严小东. 一株鸭源H1N6亚型禽流感病毒的分离鉴定及HA和NA基因序列分析[J]. 《中国畜牧兽医》, 2018, 45(2): 310-319.
[13]	程邓芳, 王红均, 黄庆洲, 张莉, 刘娟, 先宏. 海藻硫酸多糖和中药复方对鸡胚接种H9N2亚型禽流感病毒的作用[J]. 《中国畜牧兽医》, 2018, 45(12): 3594-3600.
[14]	万润, 华敏, 龙立书, 贺欣薇, 罗引幸, 周碧君, 王开功, 程振涛, 文明. H6N6亚型禽流感病毒N6基因的原核表达与多克隆抗体制备[J]. 《中国畜牧兽医》, 2018, 45(11): 2989-2995.
[15]	何莹, 谢芝勋, 罗思思, 李孟, 谢丽基, 邓显文, 谢志勤, 奉彬, 黄娇玲, 张艳芳. 禽流感病毒与鸡细小病毒二重PCR检测方法的建立[J]. 《中国畜牧兽医》, 2017, 44(7): 2126-2131.