中国畜牧兽医

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青海省部分地区3种牛病毒性腹泻病原的感染情况调查

权英存1,刘虎守2   

  1. 1.青海省大通县桥头镇新城兽医站,青海大通  8101002.青海省大通县农牧局,青海大通  810100

  • 收稿日期:2013-11-27 出版日期:2014-05-20 发布日期:2014-06-25
  • 作者简介:权英存(1983—),女,青海人,大专,助理兽医师,研究方向:动物医学。
  • 基金资助:

    青海省大通县农技推广补助项目。

Survey of Three Kinds of Bovine Viral Diarrhea Pathogens Infection in Some Areas of Qinghai Province

QUAN Ying-cun1,LIU Hu-shou2     

  1. 1.New Town Animal Husbandry and Veterinary Station of Qiaotou Datong County in Qinghai Province, Datong 810100China2.Datong County Agricultural Bureau of Qinghai ProvinceDatong 810100China

  • Received:2013-11-27 Online:2014-05-20 Published:2014-06-25

摘要: 为了解青海省部分牛群中牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)、牛轮状病毒(bovine rotavirus,BRV)和牛冠状病毒(bovine coronavirus,BCV)3种牛病毒性腹泻病原的感染现状,本研究采用RT-PCR方法首次对2012~2013年青海省部分地区的32份具有腹泻症状的临床病料及152份健康牛粪便样品进行了BVDV、BRV、BCV的核酸检测与分析。结果显示,32份腹泻牛病料样品中BVDV、BRV、BCV的阳性率分别为65.63%(21/32)、18.75%(6/32)、34.38%(11/32),且存在2种或3种病原的混合感染;152份健康牛粪便样品中BVDV、BRV、BCV的阳性率分别为3.95%(6/152)、1.97%(3/152)、0(0/152)。该结果表明青海省部分牛群中普遍存在BVDV、BRV、BCV的感染,且混合感染现象严重,需进一步加强青海省地区牛病毒性腹泻病原的综合防控。

关键词: 牛病毒性腹泻病毒; 牛轮状病毒; 牛冠状病毒; 感染; 调查

Abstract: In order to understand the infection status of 3 kinds of bovine viral diarrhea pathogens,bovine viral diarrhea virus (BVDV),bovine rotavirus (BRV) and bovine coronavirus (BCV) in Qinghai,RT-PCR method was adopted for nucleic acid detection and analysis of BVDV,BRV,BCV on clinical materials 32 with diarrhea and 152 healthy cattle feces samples which collected from 2012 to 2013 in part area of Qinghai province.The results showed that 32 diarrhea disease of cattle feed samples BVDV,BRV,BCV positive rate were 65.63% (21/32),18.75% (6/32),34.38% (11/32),respectively, mixed with 2 or 3 species infection.152 healthy cattle feces samples BVDV,BRV,BCV positive rate were 3.95% (6/152),1.97% (3/152),0(0/152),respectively.These results indicated that BVDV,BRV,BCV widespread infection in cattle in some areas of Qinghai,and mixed infection was serious,which need to further strengthen the comprehensive prevention and control of bovine viral diarrhea pathogens in Qinghai province.

Key words:

bovine viral diarrhea virus; bovine rotavirus; bovine coronavirus; infection; investigation