中国畜牧兽医

• 疾病防治 • 上一篇    下一篇

鬼针草不同溶媒提取物对耐药大肠杆菌抑菌效果初探

郑艳青,邹知明,何家康,宋剑武,司红彬   

  1. (广西大学动物科学技术学院,广西南宁 530005)
  • 收稿日期:2013-08-01 出版日期:2014-03-20 发布日期:2014-05-15
  • 通讯作者: 司红彬(1977—),男,河南人,博士,副教授,主要从事细菌耐药性研究。
  • 作者简介:郑艳青(1989—),女,广西人,硕士生,研究方向:中兽药。
  • 基金资助:

    广西自然科学基金青年基金(2012GXNSFBA053052)。

Antibacterial Study of Different Bidens bipinnata L Extracts against Drug-resistant E.coli

ZHENG Yan-qing, ZOU Zhi-ming, HE Jia-kang, SONG Jian-wu, SI Hong-bin   

  1. College of Animal Science and Technology, Guangxi University, Nanning 530005, China)
  • Received:2013-08-01 Online:2014-03-20 Published:2014-05-15

摘要: 采用微量稀释法和中西药联合传代法,比较鬼针草不同溶媒提取液对产超广谱β-内酰胺酶(ESBLs)大肠杆菌的抑菌效果,结果表明,鬼针草水煮液、乙酸乙酯提取液、丙酮提取液、三氯甲烷提取液与西药联合使用时均具有良好的抑菌效用,且抑菌效果乙酸乙酯提取液>水煮液>丙酮提取液>三氯甲烷提取液,但水煮液与抗生素联合效果更好,能降低更多不同类抗生素最小抑菌浓度(MIC)。

关键词: 鬼针草; 溶剂提取物; 产超广谱β -内酰胺酶大肠杆菌; 抑菌效果

Abstract: This paper researched antibacterial effect of different Bidens bipinnata L extracts against clinical ESBLs E.coli by means of micro-dilution assay and the present method of combining traditional Chinese herbs with western medicine in vitro. The results showed that it had good inhibitory effect under Bidens bipinnata L decoction, ethyl acetate extracts, acetone extracts and trichloromethane extracts in combination with antibiotic. And ethyl acetate extracts was the most efficient. Bidens bipinnata L decoction was the most widespread, and could reduce the MIC of different antibiotics.

Key words: Bidens bipinnata L; solvent extracts; ESBLs E.coli; antibacterial effect