禽呼肠孤病毒动物感染模型的建立

中国畜牧兽医

禽呼肠孤病毒动物感染模型的建立

毛雅元，王寿山，张立霞，康亚男，陈冰，吕茂杰，何召庆，杨保收

（天津瑞普生物技术股份有限公司,瑞普生物研究院,天津 300308）

出版日期:2014-01-20 发布日期:2014-02-27
通讯作者: 何召庆。E-mail:zhqhe111@sina.com
作者简介:毛雅元(1984—)，女，黑龙江人，硕士，研究方向：分子生物学及动物疫苗。
基金资助:
瑞普研究院鸡病毒性关节炎灭活疫苗专项基金（201209130405）。

Establishment of Animal Model Infected by Avian Reovirus

MAO Ya-yuan, WANG Shou-shan, ZHANG Li-xia, KANG Ya-nan, CHEN Bing, LV Mao-jie, HE Zhao-qing, YANG Bao-shou

(Ringpu Biological Research Institute, Tianjin Ringpu Bio-technology Co., Ltd., Tianjin 300308，China)

Online:2014-01-20 Published:2014-02-27

摘要/Abstract

摘要： 本试验旨在建立鸡病毒性关节炎动物感染模型，为疫苗效力检验奠定基础。试验采用禽呼肠孤病毒（avian reovirus，ARV）AV2311强毒株和ARV T98强毒株，分别以10^2.0、10^3.0、10^3.5、10^4.5和10^5.5 ELD₅₀/0.2 mL剂量感染49日龄SPF鸡，感染后观察10 d，每天观察、检查接种鸡足垫、小腿或跗关节的肿胀、跛行及红肿情况，于感染后6 d采血，感染后10 d剖检，应用ELISA检测方法测定血清抗体。结果显示，10^5.5和10^4.5 ELD₅₀/0.2 mL感染剂量鸡发病率均达100%，鸡足垫肿胀严重，呈暗红色或紫色，AV2311株症状稍轻于T98株；10^3.5 ELD₅₀/0.2 mL感染剂量鸡发病率为90%，10^3.0和10^2.0 ELD₅₀/0.2 mL感染剂量鸡发病率为80%。ELISA检测结果显示，2毒株10^4.5和10^5.5 ELD₅₀/0.2 mL感染剂量血清抗体均为阳性。试验结果表明，ARV T98株毒力强，具有好的免疫原性，最佳感染剂量为10^4.0 ELD₅₀/0.2 mL，为鸡病毒性关节炎疫苗质量标准的研究提供了重要依据。

关键词: 禽呼肠孤病毒; 动物感染模型; 肿胀; 跛行

Abstract: The infection model of avian reovirus in chicken was established, which layed the ground for vaccine efficacy test.The 49-day-old chickens were inoculated with 10²^.⁰, 10³^.⁰, 10³^.⁵, 10⁴^.⁵and 10⁵^.⁵ ELD₅₀/0.2 mL of two strains of ARV, T98 and AV2311, respectively, in foot pad inoculation method. Clinical signs were observed ten days and recorded daily. 6 days after inoculation, serum samples were taken. 10 days after inoculation, chickens were sacrificed for necropsy. Serum antibody was detected by ELISA. The results indicated that the morbidity of 10⁵^.⁵and 10⁴^.⁵ ELD₅₀/0.2 mL were 100%, and the foot pads of chicken were swelled severely, which were dark red or purple. Symptoms of AV2311 set was a bit lighter than that of T98 strain. The morbidity of 10³^.⁵ ELD₅₀/0.2 mL was 90% and the morbidity of 10³^.⁰and 10²^.⁰ ELD₅₀/0.2 mL were 80%. The ELISA result indicated that only the serum efficacy of 10⁴^.⁵and 10⁵^.⁵ ELD₅₀/0.2 mL set of two strains of ARV were positive.The experiment proved that the virulence of ARV T98 strain was strong, and had good immunogenicity. The best inoculated dose of ARV T98 strain was 10⁴^.⁰ ELD₅₀/0.2 mL, which provided the important basis for researching the quality standard of chicken viral arthrilis vaccine.

Key words: avian reovirus; animal infection model; swell; limp

毛雅元，王寿山，张立霞，康亚男，陈冰，吕茂杰，何召庆，杨保收. 禽呼肠孤病毒动物感染模型的建立[J]. 中国畜牧兽医.

MAO Ya-yuan, WANG Shou-shan, ZHANG Li-xia, KANG Ya-nan, CHEN Bing, LV Mao-jie, HE Zhao-qing, YANG Bao-shou. Establishment of Animal Model Infected by Avian Reovirus[J]. China Animal Husbandry & Veterinary Medicine.

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