腹腔注射白消安对小鼠生殖能力影响的研究

›› 2013, Vol. 40 ›› Issue (12): 142-145.

腹腔注射白消安对小鼠生殖能力影响的研究

王琛^1,2, 朱化彬², 秦玉圣², 刘玲², 郝海生², 赵学明², 秦彤², 刘建华³, 黎宗强¹, 王栋²

1. 广西大学动物科学技术学院, 广西南宁 530004;
2. 中国农业科学院北京畜牧兽医研究所, 农业部畜禽遗传资源与利用重点开放实验室, 北京 100193;
3. 内蒙赤峰市敖汉旗畜牧业局动物卫生监督所, 内蒙古赤峰 024300

收稿日期:2013-05-14 出版日期:2013-12-20 发布日期:2014-02-11
通讯作者: 王栋, 黎宗强 E-mail:dwangcn2002@vip.sina.com.cn;lizq20@163.com
作者简介:王琛（1987- ），女，辽宁人，硕士，研究方向：繁殖育种。
基金资助:
国家十二五科技支撑计划课题（2011BAD19B02）。

Study on Effect of Fertility after Mice Treated with Busulfan by Intra-peritoneal Injection

WANG Chen^1,2, ZHU Hua-bin², QIN Yu-sheng², LIU Ling², HAO Hai-sheng², ZHAO Xue-ming², QIN Tong², LIU Jian-hua³, LI Zong-qiang¹, WANG Dong²

1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;
2. The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Husbandry Bureau of Aohan County, Chifeng City, Inner Monggolia Municipality, Chifeng 024300, China

Received:2013-05-14 Online:2013-12-20 Published:2014-02-11

摘要/Abstract

摘要： 本研究为了探索白消安对小鼠生殖能力的影响，将20只6~7周龄性成熟雄ICR鼠随机分成4组，以注射50%DMSO作为空白对照，分别以每只40、45、50 mg/kg剂量腹腔注射白消安。从注射当天开始分阶段共与母鼠合笼15次，记录了460只合笼母鼠的受孕率、窝产仔数，以及922只后代的性别，并对记录数据进行统计分析，研究了不同剂量药物对小鼠生殖能力的影响。结果表明，腹腔注射白消安能够造成小鼠的短期不育，但这种不育是可以恢复的，且低剂量比高剂量注射恢复更快。白消安虽然会对小鼠的生殖能力造成影响，但不会影响其后代的性别比例。

关键词: 白消安; 腹腔注射; 生殖能力

Abstract: The effect of fertility after mice treated with busulfan was observed and evaluated in this study. 20 sexually matured male ICR mice with 6 to 7 weeks old were randomly divided into 4 groups. Mice were treated with busulfan in doses of 40,45,50 mg/kg by intra-peritoneal injection, respectively, and perform a blank determination with 50% DMSO. Busulfan-treated mice mated with female 15 times after injection in phases, and we recorded the data of 460 females used for mating, such as conception rate, the litter size and the gender of 922 offspring. Then we measured male fertility after the mice were treated with differential dose by statistical analyses. The short-term infertility result from intra-peritoneal injection of busulfan, which can be recovered by themselves. The recovery of mice fertility treated with low doses was quickly than that with higher doses. The sex ratio of the offspring did not change by the treatment, though it made an impact to fertility.

Key words: busulfan; intra-peritoneal injection; fertility

中图分类号:

S814.1

王琛, 朱化彬, 秦玉圣, 刘玲, 郝海生, 赵学明, 秦彤, 刘建华, 黎宗强, 王栋. 腹腔注射白消安对小鼠生殖能力影响的研究[J]. , 2013, 40(12): 142-145.

WANG Chen, ZHU Hua-bin, QIN Yu-sheng, LIU Ling, HAO Hai-sheng, ZHAO Xue-ming, QIN Tong, LIU Jian-hua, LI Zong-qiang, WANG Dong. Study on Effect of Fertility after Mice Treated with Busulfan by Intra-peritoneal Injection[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(12): 142-145.

参考文献

1 张茨,王玲珑,金化民,等.白消安诱导小鼠无精子症模型[J].医学新知杂志,2003,4(13):201~203.
2 Brinster R,Zimmermann J. Spermatogenesis following male germ-cell transplantation[J]. Proc Natl Acad Sci USA,1994,91(24):11298~11302.
3 Bucci L, Meistrich M. Effects of busulfan on murine spermatogenesis:Cytotoxicity,sterility, sperm abnormalities,and dominant lethal mutations[J].Mutat Res-Fund Mol M,1987,176:259~268.
4 Choi Y, Ok D, Kwon D N,et al. Murine male germ cell apoptosis induced by busulfan treatment correlates with loss of c-kit-expression in a Fas/FasL- and p53-independent manner[J].FEBS Lett, 2004,575 (1~3):41~51.
5 Heller R,Jones J.Production of ovarian dysgenesis in the rat and human by busulphan[J].Am J Obstet Gynecol,1964,89:414~420.
6 Hemsworth B,Jackson H.Effect of busulfan on the fetal gonad[J].Nature,1962,195:816~817.
7 Kramer M,de Rooij D. Spermatogonial stem cell renewal in the rat, mouse and golden hamster.A study with the alkylating agent myleran[J].Z Zellforsch Mikrosk Anat,1968,92:400~405.
8 Marcon L,Zhang X F, Barbara F,et al.Effects of chemotherapeutic agents for testicular cancer on rat spermatogonial stem/progenitor cells[J].J Androl,2011,32(4):432~443.
9 Ogawa T, Dobrinski I, Brinster R. Recipient preparation is critical for spermatogonial transplantation in the rat[J]. Tissue & Cell,1999,31(5):461~472.
10 Pérez-Crespo M, Pericuesta E, Pérez-Cerezales, et al. Effect of liver growth factor on both testicular regeneration and recovery of spermatogenesis in busulfan-treated mice[J].Reprod Biol Endocrinol,2011,9:21.
11 Rockett J,Mapp F,Garges J,et al.Effects of hyperthermia on spermatogenesis, apoptosis, gene expression, and fertility in adult male mice[J].Biol Reprod,2001,65(1):229~239.
12 Sanders J, Hawley J,Levy W.Pregnancies following high dose cyclophosphamide with or without high dose busulfan or total body irradiation and bone marrow transplantation[J]. Blood,1996,87:3045~3052.
13 Shinohara T,Orwiq K E, Avarbock M R, et al.Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells[J].Proc Natl Acad Sci USA,2000,97(15):8346~8351.
14 Shiraishi A,Sakumi K,Sekiguchi M,et al.Increased susceptibility to chemotherapeutic alkylating agents of mice deficient in DNA repair methyltransferase[J]. Carcinogenesis,2000,21(10):1879~1883.
15 Smith R,Lowe G,Kavoussi P,et al.Confocal fluorescence microscopy in a murine model of microdissection testicular sperm extraction to improve sperm retrieval[J].J Urol,2012, 187(5):1918~1923.
16 Zohni K,Zhang X,Tan S L,et al. The efficiency of male fertility restoration is dependent on the recovery kinetics of spermatogonial stem cells after cytotoxic treatment with busulfan in mice[J].Hum Reprod,2012,27(1):44~53.

[1]	吴开慧, 周成利, 赵羚均, 许师源, 刘松奇, 董智豪, 原开敏, 王栋. 白消安致小鼠睾丸损伤的机理及其调控通路的研究进展[J]. 中国畜牧兽医, 2023, 50(7): 2811-2819.
[2]	李佳豪, 李超, 臧长江, 孟军, 李宏坤, 贾怡琪, 麦丽克扎提·艾尔肯, 唐纳尔·托留别克. 灌胃和腹腔注射鼠李糖乳杆菌对妊娠期小鼠粪便菌群多样性的影响[J]. 中国畜牧兽医, 2023, 50(1): 151-160.
[3]	唐光武;吴玉臣;郭爽;闫若潜. 仔猪黄痢病原分离株药敏试验和不同给药方法疗效比较研究[J]. , 2010, 37(2): 159-161.