关键词: 山羊痘病毒; 分离; 聚合酶链式反应; 鉴定

Abstract: A strain of goat pox viruses was isolated from suspected goat pox cases in Inner Mongolia province, and was named as NM strain. Samples from skin varioliform and blisters were collected and inoculated BHK-21 cells and 10-day-old SPF chicken. Typical cytopathic effect was observed in BHK-21 cells, significant variola was observed in vaccination site. The NM strain cytotoxic and embryo virus could be inhibited by goat pox virus positive serum. Using goat pox virus specific detection primer amplified of the NM strains by PCR, obtained consistent with the expected size of 445 bp fragment. Then the amplification product was cloned into pMD18-T vector, after identifying with double-enzyme cutting. The sequencing of positive clones showed that the NM strain fragment had a high degree of homology with published goat pox virus, the homology was 99.2% to 100%.

Key words: goat pox viruses; isolation; PCR; identification