影响猪卵母细胞胞质内单精子注射效果的因素

中国畜牧兽医

影响猪卵母细胞胞质内单精子注射效果的因素

张立苹, 刘西梅, 李莉, 肖红卫, 乔宪凤, 郑新民

湖北省农业科学院畜牧兽医研究所/胚胎工程与分子育种湖北省重点实验室,湖北武汉 430064

收稿日期:2012-03-23 出版日期:2012-09-20 发布日期:2012-09-18
通讯作者: 郑新民,男,研究员,研究方向:动物生物技术。E-mail:anbit20@163.com E-mail:anbit20@163.com
作者简介:张立苹(1982－),女,河北人,助理研究员,研究方向:动物胚胎工程。
基金资助:
转基因生物新品种培育专项(2011ZX08006-003、2011ZX08006-002),重点实验室开放课题(2012ZD164)。

The Influencing Factors of the Efficiency of Intracytoplasmic Sperm Injection in Porcine Oocytes

ZHANG Li-ping, LIU Xi-mei, LI Li, XIAO Hong-wei, QIAO Xian-feng, ZHENG Xin-min

Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Science, Wuhan 430064,China

Received:2012-03-23 Online:2012-09-20 Published:2012-09-18

摘要/Abstract

摘要： 本试验探讨了成熟培养液中不同添加物、精子的处理方法及胞质内单精子注射(intracytoplasmic sperm injection,ICSI)胚胎激活方法3个方面,研究了影响猪卵母细胞胞质内单精子注射效果的因素。结果表明,在体外成熟液中添加30 ng/mL上表皮生长因子(epidermal growth factor,EGF)可以明显促进ICSI胚胎的后续发育(P＜0.05);精子的超声波处理和显微操作处理两种方法对ICSI受精卵发育的影响不明显(P＞0.05);电激活比较适合于ICSI胚胎的激活。

关键词: 胞质内单精子注射; 猪卵母细胞; 受精率

Abstract: The objective of this study was taken to investigate the effects of different additive of maturation media and sperm pretreatment and activation methods on ICSI embryos. The results showed that the development of ICSI embryos got improving obviously by adding 30 ng/mL EGF in maturation medium(P＜0.05),it had no significant effect for ICSI embryos between ultrasonic treatment and microinjection in sperm(P＞0.05), and the electro-activation was suitable for ICSI embryos.

Key words: ICSI; porcine oocyte; fertilization rate

中图分类号:

S814.4

张立苹, 刘西梅, 李莉, 肖红卫, 乔宪凤, 郑新民. 影响猪卵母细胞胞质内单精子注射效果的因素[J]. 中国畜牧兽医.

ZHANG Li-ping, LIU Xi-mei, LI Li, XIAO Hong-wei, QIAO Xian-feng, ZHENG Xin-min. The Influencing Factors of the Efficiency of Intracytoplasmic Sperm Injection in Porcine Oocytes[J]. .

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