卵母细胞核因子促进核移植四倍体胚胎早期发育研究

doi:10.16431/j.cnki.1671-7236.2017.01.020

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (1): 148-154.doi: 10.16431/j.cnki.1671-7236.2017.01.020

卵母细胞核因子促进核移植四倍体胚胎早期发育研究

付博¹, 刘娣^1,2, 马红¹, 郭镇华¹, 汪亮¹, 李忠秋¹, 彭福刚¹, 张冬杰¹, 刘自广¹, 白晶³

1. 黑龙江省农业科学院畜牧研究所, 哈尔滨 150086;
2. 东北农业大学动物科学技术学院, 哈尔滨 150030;
3. 黑龙江中医药大学现代教育信息中心, 哈尔滨 150040

收稿日期:2016-06-18 出版日期:2017-01-20 发布日期:2017-01-19
通讯作者: 刘娣 E-mail:liudi1963616@163.com
作者简介:付博(1978-),男,黑龙江哈尔滨人,博士,副研究员,研究方向:猪胚胎工程与发育生物学,E-mail:fubohao810@163.com
基金资助:
黑龙江省自然科学基金（QC2013C021）；黑龙江省博士后科研启动金项目（LBH-Q15130）；黑龙江省农业科学院引进博士研究项目（201507-32）；国家自然科学基金（31201804）

Study on Oocyte Nuclear Factor Promotes the Early Development of Tetraploid Somatic Cell Nuclear Transfer Embryos

FU Bo¹, LIU Di^1,2, MA Hong¹, GUO Zhen-hua¹, WANG Liang¹, LI Zhong-qiu¹, PENG Fu-gang¹, ZHANG Dong-jie¹, LIU Zi-guang¹, BAI Jing³

1. Institute of Animal Husbandry Research, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;
2. College of Animal Science, Northeast Agricultural University, Harbin 150030, China;
3. Modern Education Technology and Information Center, Heilongjiang University of Chinese Medicine, Harbin 150040, China

Received:2016-06-18 Online:2017-01-20 Published:2017-01-19

摘要/Abstract

摘要：

研究旨在探讨猪卵母细胞核因子在重编程过程中发挥的作用。将体细胞引入未去核的MⅡ期卵母细胞中，构建体细胞核与卵母细胞核共存的核移植四倍体胚胎。通过分析核移植四倍体胚胎的早期发育情况探讨卵母细胞核因子对核移植四倍体胚胎早期发育的影响。结果显示，核移植四倍体胚胎、孤雌二倍体胚胎及孤雌单倍体胚胎这3组胚胎的卵裂率极显著高于核移植二倍体胚胎（P<0.01），且核移植四倍体囊胚率及总细胞数也极显著高于核移植二倍体囊胚（P<0.01）。与通过标准核移植程序构建的核移植二倍体胚胎相比，核移植四倍体胚胎具有更强的发育能力。本研究建立了一个体细胞核与完整卵母细胞核因子物质共存的四倍体胚胎模型，有助于研究供体核与卵母细胞核之间的联系，为研究核因子在重编程过程中发挥的作用提供了平台。

关键词: 克隆效率; 核因子; 猪卵母细胞; 体细胞核移植; 四倍体

Abstract:

The study was aimed to investigate the role of porcine oocyte nuclear factors during reprogramming. Somatic cell nuclei was introduced into intact MⅡ oocytes to establish tetraploid somatic cell nuclear transfer (SCNT) embryos containing both somatic nuclei and oocyte nuclei. And then the influence of the oocyte nucleus on tetraploid SCNT embryo development was examined by assessing characteristics including cleavage rate and blastocyst rate. The results showed that the cleavage rate of tetraploid SCNT embryos,diploid parthenogenetic embryos and haploid parthenogenetic embryos was extremely significantly higher than that of standard diploid SCNT embryos (P<0.01). The blastocyst rate and the total number of cells in tetraploid SCNT embryos were extremely significantly higher than that of standard diploid SCNT embryos (P<0.01).Overall,tetraploid SCNT embryos had a higher developmental competence than standard diploid SCNT embryos. In conclusion, the embryonic model was established in which a fetal fibroblast nucleus and an oocyte M Ⅱ plate coexist. Tetraploid SCNT represented a new research platform that was potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.

Key words: cloning efficiency; nuclear factors; porcine oocytes; somatic cell nuclear transfer; tetraploid

中图分类号:

Q813.7

付博, 刘娣, 马红, 郭镇华, 汪亮, 李忠秋, 彭福刚, 张冬杰, 刘自广, 白晶. 卵母细胞核因子促进核移植四倍体胚胎早期发育研究[J]. 《中国畜牧兽医》, 2017, 44(1): 148-154.

FU Bo, LIU Di, MA Hong, GUO Zhen-hua, WANG Liang, LI Zhong-qiu, PENG Fu-gang, ZHANG Dong-jie, LIU Zi-guang, BAI Jing. Study on Oocyte Nuclear Factor Promotes the Early Development of Tetraploid Somatic Cell Nuclear Transfer Embryos[J]. , 2017, 44(1): 148-154.

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卵母细胞核因子促进核移植四倍体胚胎早期发育研究

Study on Oocyte Nuclear Factor Promotes the Early Development of Tetraploid Somatic Cell Nuclear Transfer Embryos

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