牦牛源产细菌素屎肠球菌SWUN5732基因组测序及抑菌相关基因分析

doi:10.16431/j.cnki.1671-7236.2023.11.023

摘要/Abstract

摘要： 【目的】本研究旨在通过研究1株牦牛源产细菌素屎肠球菌SWUN5732的基因组序列，发掘该菌种的关键功能性特征基因。【方法】通过Illumina PE150测序系统，对屎肠球菌SWUN5732进行基因组测序，并在GO、KEGG、COG和NR数据库对基因组的基本功能特征进行注释，结合NR数据库的注释，挖掘出基因组中潜在的抗菌、抗氧化和抑菌肽相关基因。【结果】屎肠球菌SWUN5732的基因组大小为2 824 168 bp，有效平均GC含量约为38.09%；可检测到的编码基因数量约为2 919个，全部编码基因总长度约为2 387 787 bp，平均长度约为818 bp。在GO数据库中SWUN5732基因组一共有8 851个基因得到注释，分别注释到分子功能、细胞组分和生物过程三大类45个条目；在KEGG数据库中SWUN5732基因组共有1 534个基因被注释，分别为细胞过程、环境信息处理、遗传信息处理、人类疾病、新陈代谢和生物体系统6大功能38个通路；在COG数据库中，SWUN5732基因组共有2 118个基因被注释，在基因组中还包含4个有ABC型氨基酸转运系统（渗透酶组分）的功能序列和具有重复ATP酶结构域的ABC转运蛋白的序列；通过NR数据库对屎肠球菌SWUN5732的功能注释，一共有2 844个基因得到注释，发现SWUN5732基因组中包含抗菌物质、抗氧化物质和抑菌肽类相关基因。系统进化树分析结果表明，该菌株与ATCC 19434的进化关系最为接近。【结论】本研究完成对牦牛源产细菌素屎肠球菌SWUN5732基因组测序和潜在抑菌物质挖掘，证实该菌株具有耐酸耐胆盐环境适应性并探究了抗氧化、免疫调控、产细菌素和抗菌物质相关基因，为此菌株作为抗生素替代品或饲料益生菌添加剂提供了可靠的依据。

关键词: 牦牛酸奶; 屎肠球菌; 基因测序; 基因功能注释; 细菌素

Abstract: 【Objective】 The objective of this study was to investigate the essential functional genes of Enterococcus faecium SWUN5732, a strain isolated from yak, through an analysis of its genome sequence detection outcomes.【Method】 Through Illumina PE150 sequencing system, the genome of Enterococcus faecium SWUN5732 was sequenced, and the basic functional characteristics of the genome were annotated in GO, KEGG, COG and NR databases.Combined with the annotation of NR database, the potential antibacterial, antioxidant and antibacterial peptide related genes in the genome were excavated.【Result】 The genome size of Enterococcus faecium SWUN5732 was 2 824 168 bp, and the effective average GC-content was about 38.09%.The number of detectable coding genes was approximately 2 919, and the total length of all coding genes was approximately 2 387 787 bp, with an average length of approximately 818 bp.In the GO database, a total of 8 851 genes in the SWUN5732 genome were annotated, with 45 entries in three major categories:Molecular function, cellular component and biological process.In the KEGG database, there were a total of 1 534 genes annotated in the SWUN5732 genome, including 38 pathways for six major functions:Cellular processes, environmental information processing, genetic information processing, human diseases, metabolism, and biological systems.In the COG database, a total of 2 118 genes had been annotated in the SWUN5732 genome, which also included four functional sequences with ABC type amino acid transport systems (osmotic enzyme components) and sequences of ABC transport proteins with repetitive ATPase domains.According to the functional annotation of Enterococcus faecium SWUN5732 in NR database, a total of 2 844 genes were annotated, and it was found that the genome of SWUN5732 contained antibacterial substances, antioxidant substances and antibacterial peptides related genes.The phylogenetic tree analysis results indicated that the strain had the closest evolutionary relationship to ATCC 19434.【Conclusion】 In this study, the genome of bacteriocin-producing Enterococcus faecium SWUN5732 from yaks was sequenced and potential antimicrobial substances were excavated, which confirmed that the strain had acid and bile salt tolerance environmental adaptability and explored the genes related to antioxidant, immune regulation, bacteriocin-production and antimicrobial substances, providing a reliable basis for the strain as an antibiotic substitute or feed probiotic additive.

Key words: yak yogurt; Enterococcus faecium; genome sequencing; gene function annotation; bacteriocin

中图分类号:

S852.6

蒲思成, 郑惠滨, 冉旋, 杨发龙, 汤承, 刘媛媛, 陈德纯. 牦牛源产细菌素屎肠球菌SWUN5732基因组测序及抑菌相关基因分析[J]. 中国畜牧兽医, 2023, 50(11): 4545-4556.

PU Sicheng, ZHENG Huibin, RAN Xuan, YANG Falong, TANG Cheng, LIU Yuanyuan, CHEN Dechun. Genome Sequencing and Antimicrobial Related Gene Analysis of Bacteriocin-producing Enterococcus faecium SWUN5732 from Yak[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4545-4556.

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