内蒙古鼠疫自然疫源地鼠疫菌耐药相关基因检测及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2023.11.021

摘要/Abstract

摘要： 【目的】试验旨在对内蒙古鼠疫自然疫源地鼠疫耶尔森菌（Yersinia pestis，鼠疫菌）抗生素及消毒剂耐药基因进行检测及生物信息学分析，以期为该地区的鼠疫防控提供科学依据。【方法】利用PCR对60株内蒙古鼠疫自然疫源地鼠疫菌的DNA进行耐链霉素耐药基因（StrA、StrB）、β-内酰胺类耐药基因（Tem、Ctx-m）、磺胺类耐药基因（Sul1、Sul2）和季铵盐类消毒剂耐药基因（QacEdelta）检测，并预测StrA、Sul1和QacEdelta蛋白的结构功能及抗原表位。【结果】PCR扩增结果显示，阴性对照和阳性对照均成立，60株鼠疫菌扩增结果均为阴性。从内蒙古鼠疫自然疫源地分离的鼠疫菌株中未发现有耐链霉素、磺胺类、β-内酰胺类抗菌药物及耐消毒剂等相关基因的菌株。生物信息学分析表明，StrA、Sul1和QacEdelta蛋白均为不含有跨膜区的疏水性外膜蛋白，且均属于分泌蛋白。3种蛋白的二级结构均以α-螺旋为主，占比分别为37.83%、45.88%和46.09%。抗原表位预测显示，3种蛋白中B细胞表位分别为9、8和3个；T细胞表位分别为9、16和14个。【结论】内蒙古鼠疫自然疫源地分离的60株鼠疫菌均未检测到耐药及耐消毒剂相关基因。StrA、Sul1和QacEdelta蛋白含有较多的B、T细胞抗原表位，具有较好的抗原性。本试验结果为指导该疫源地的鼠疫防控工作中合理使用抗菌药物和消毒剂提供参考依据。

关键词: 内蒙古; 鼠疫菌; 消毒剂及耐药基因; 生物信息学

Abstract: 【Objective】 This study was aimed to detect the antibiotic and disinfectant resistance-related genes of Yersinia pestis in Inner Mongolia natural plague foci, and carry out bioinformatics analysis, so as to provide scientific basis for plague prevention and control in this region.【Method】 PCR method was used to detect the streptomycin resistance genes (StrA and StrB), beta-lactam resistance genes (Tem and Ctx-m), sulfanilamide resistance gene (Sul1 and Sul2) and quaternary ammonium salt resistance gene (QacEdelta) in 60 strains of Yersinia pestis isolated from Inner Mongolia natural plague foci.At the same time, the structure, function and antigenic epitopes of StrA, Sul1 and QacEdelta proteins were predicted.【Result】 PCR amplification results showed that the negative and positive controls were established, and the amplification results of 60 strains of Yersinia pestis were negative.No genes related to streptomycin resistance, sulfonamides resistance, beta-lactam resistance and disinfectant resistance were found in the plague strains isolated from Inner Mongolia natural plague foci.Bioinformatics analysis showed that StrA, Sul1 and QacEdelta proteins were all hydrophobic outer membrane proteins that did not contain transmembrane regions, and were secreted proteins.The secondary structure of the three proteins was dominated by alpha helix, which accounted for 37.83%, 45.88% and 46.09%, respectively.Antigen epitope prediction showed that the three proteins contained 9, 8 and 3 B cell antigenic epitopes, and 9, 16 and 14 T cell antigenic epitopes, respectively.【Conclusion】 60 strains of Yersinia pestis isolated from Inner Mongolia plague foci did not have the characteristics of drug resistance and disinfectant resistance related genes.StrA, Sul1 and QacEdelta proteins contained more B and T cell antigenic epitopes and had good antigenicity.The results provided a reference for the rational use of antibiotics and disinfectants in plague prevention and control in these foci.

Key words: Inner Mongolia; Yersinia pestis; disinfectant and drug resistance genes; bioinformatics

中图分类号:

S816.6

王姝懿, 李建云, 刘芳, 冯一兰, 韩冰, 范蒙光. 内蒙古鼠疫自然疫源地鼠疫菌耐药相关基因检测及生物信息学分析[J]. 中国畜牧兽医, 2023, 50(11): 4526-4535.

WANG Shuyi, LI Jianyun, LIU Fang, FENG Yilan, HAN Bing, FAN Mengguang. Detection and Bioinformatics Analysis of Genes Related to Drug Resistance of Yersinia pestis in Inner Mongolia Natural Plague Foci[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4526-4535.

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