复方中药对腹水综合征肉鸡肺组织炎症-增殖的调控研究

doi:10.16431/j.cnki.1671-7236.2023.08.035

摘要/Abstract

摘要： 【目的】基于缺氧-炎症-内皮增殖理论,探讨腹水综合征肉鸡肺组织的发病机理,并研究复方中药的防治机制。【方法】选取150羽8日龄健康AA肉鸡,随机分为5组,空白对照组常规饲养;肉鸡腹水综合征模型组采用低温、高能饲料、高钠饮水多因素法造模;在模型组基础上,高、中、低剂量复方中药组于每日饮水中按体重分别添加2.0、1.0、0.5 g/kg复方中药。观察临床症状和剖检变化;采用全自动血细胞分析仪检测血液白细胞计数;利用实时荧光定量PCR检测肺组织中缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)、血管细胞黏附分子1(vascular cell adhesion molecule 1,VCAM1)、Sprouty相关EVH1域含蛋白质1(Sprouty-related,EVH1 domain-containing protein 1,SPRED1)、磷脂酰肌醇-3激酶调节亚单位2(phosphatidylinositol 3-kinase regulatory subunit 2,PIK3R2)基因及miRNA-126-3p的表达量;采用ELISA法检测肺组织中VCAM1、SPRED1、PIK3R2蛋白表达量。【结果】与空白对照组相比,模型组肉鸡精神萎靡,腹部膨大,剖检可见腹腔内有大量腹水,肺脏、肝脏肿大充血,有心包积液;25、35、45、55日龄模型组肉鸡血液中白细胞计数极显著升高(P＜0.01);35日龄模型组肉鸡肺组织中HIF-1α基因表达量极显著升高(P＜0.01);15、25、35、45日龄模型组肉鸡肺组织中VCAM1基因和蛋白表达量均极显著升高(P＜0.01);15日龄模型组肉鸡肺组织中SPRED1基因和PIK3R2蛋白表达量均极显著降低(P＜0.01);25、35、45日龄模型组肉鸡肺组织中SPRED1、PIK3R2基因和蛋白表达量均极显著降低(P＜0.01);35日龄模型组肉鸡肺组织中miRNA-126-3p相对表达量极显著升高(P＜0.01)。与模型组相比,复方中药各剂量组肉鸡上述结果均有不同程度改善,其中复方中药高剂量组各指标变化最为明显。【结论】肺组织缺氧可引发炎症及增殖异常,参与了肉鸡腹水综合征的发生发展;复方中药可有效防治肉鸡腹水综合征,且2.0 g/mL复方中药作用效果最好,其作用机制与调节肺组织缺氧、炎症、增殖密切相关。

关键词: 肉鸡腹水综合征; 复方中药; 缺氧; 炎症; 增殖

Abstract: 【Objective】 Based on the theory of hypoxia-inflammation-endothelial proliferation,the pathogenesis of lung in broilers with ascites syndrome was explored,and the preventive and therapeutic mechanism of compound traditional Chinese medicine was studied.【Method】One hundred and fifty 8-day-old healthy AA broilers were randomly divided into 5 groups and fed routinely in blank control group.The ascites syndrome model group was established by multi-factor method of low temperature,high energy feed and high sodium drinking water.On the basis of model group,2.0,1.0 and 0.5 g/kg of compound traditional Chinese medicine were added to the daily drinking water of high,medium and low dose groups according to their body weight,respectively.The changes of clinical symptoms and anatomy were observed,the white blood cell count was detected by automatic hematology analyzer,the expression of hypoxia-inducible factor-1α(HIF-1α),vascular cell adhesion molecule 1(VCAM1),Sprouty-related,EVH1 domain-containing protein 1(SPRED1) and phosphatidylinositol 3-kinase regulatory subunit 2(PIK3R2) genes and miRNA-126-3p in lung were detected by Real-time quantitative PCR,and the expression of VCAM1,SPRED1 and PIK3R2 proteins in lung was detected by ELISA.【Result】 Compared with blank control group,broilers in model group were apathetic,had an enlarged abdomen,and on necropsy,they were found to have a large number of ascites in the abdominal cavity,pulmonary and hepatomegaly congestion,and had pericardial effusion.The white blood cell counts of broilers in model group at the age of 25,35,45 and 55 days were extremely significantly increased (P＜0.01);The expression of HIF-1α gene in lung of broilers in model group at the age of 35 days was extremely significantly increased (P＜0.01);The gene and protein expression of VCAM1 in lung of broilers in model group at the age of 15,25,35 and 45 days were extremely significantly increased (P＜0.01).The expression of SPRED1 gene and PIK3R2 protein in lung of broilers in model group at the age of 15 days were extremely significantly decreased (P＜0.01).The gene and protein expression of SPRED1 and PIK3R2 in lung of broilers in model group at the age of 25,35 and 45 days were extremely significantly decreased (P＜0.01).The expression of miRNA-126-3p in lung of broilers in model group at the age of 35 days was extremely significantly increased (P＜0.01).Compared with model group,the above results of broilers in each dose group of compound traditional Chinese medicine were improved to various degrees,and among them,the changes of each index in high dose group of compound traditional Chinese medicine were the most obvious.【Conclusion】 Inflammation and proliferation abnormalities triggered by hypoxia in lung were involved in the development of ascites syndrome in broilers.Compound traditional Chinese medicine could effectively prevent and treat ascites syndrome in broilers,among which 2.0 g/mL compound traditional Chinese medicine had the best effect.The mechanism of action was closely related to the regulation of hypoxia,inflammation,and proliferation in lung.

Key words: ascites syndrome; compound traditional Chinese medicine; hypoxia; inflammation; proliferation

中图分类号:

S858.31

崔亮, 王珂瑶, 张新平, 王慧敏, 韩宇峰, 张娟, 邓瑞强, 康杰, 段智变. 复方中药对腹水综合征肉鸡肺组织炎症-增殖的调控研究[J]. 中国畜牧兽医, 2023, 50(8): 3373-3383.

CUI Liang, WANG Keyao, ZHANG Xinping, WANG Huimin, HAN Yufeng, ZHANG Juan, DENG Ruiqiang, KANG Jie, DUAN Zhibian. Study on the Regulation of Compound Traditional Chinese Medicine on Inflammation-proliferation of Lung in Broilers with Ascites Syndrome[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3373-3383.

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