基于网络药理学探究芪翁黄柏散治疗仔猪腹泻的有效成分及作用机制

doi:10.16431/j.cnki.1671-7236.2022.12.037

摘要/Abstract

摘要： 【目的】基于网络药理学预测芪翁黄柏散治疗仔猪腹泻的有效成分，并探究其作用机制。【方法】通过TCMSP数据库筛选芪翁黄柏散潜在的有效成分和靶标蛋白，利用Cytoscape 3.9.1软件构建药物成分-靶点网络；通过GeneCards和OMIM数据库检索腹泻靶点并去重，获得疾病与药物的共同靶点，导入STRING数据库并构建蛋白互作（PPI）网络，对共同靶点进行GO功能和KEGG通路富集分析。【结果】共获得芪翁黄柏散37个有效成分和74个靶点，其中与疾病相交的共同靶点有68个；GO功能注释得到生物过程147个条目，细胞组分15个条目，分子功能32个条目；KEGG通路富集分析获得98个通路。槲皮素、山奈酚、豆甾醇、四氢小檗碱、小檗碱等为其有效成分，肿瘤坏死因子（TNF）、肿瘤蛋白P53（TP53）、血管内皮生长因子A （VEGFA）、白细胞介素6（IL6）、IL10、C-C基序趋化因子配体2（CCL2）、上皮生长因子（EGF）、核因子κB抑制剂α（NFKBIA）等为药物关键靶点，癌症、磷脂酰肌醇3-激酶/蛋白激酶B （PI3K-Akt）、丝裂原激活的蛋白激酶（MAPK）、IL17、炎症性肠病等信号通路为关键通路，还涉及到免疫应答、转录、细胞凋亡、细胞因子活性、细胞外等方面。【结论】本研究预测了芪翁黄柏散治疗仔猪腹泻的有效成分及作用机制，体现了中药多成分、多靶点、多通路的特点，并为后续研究提供方向和参考。

关键词: 网络药理学; 仔猪腹泻; 芪翁黄柏散; 信号; 通路

Abstract: 【Objective】 The purpose of this research was to predict the effective components and explore the mechanism of Qiweng Huangbo powder in treating diarrhea of piglets based on network pharmacology.【Method】 Potential active ingredients and target proteins of Qiweng Huangbo powder were screened through TCMSP database website, and Cytoscape 3.9.1 software was used to construct a drug components-targets network.GeneCards and OMIM databases were used to retrieve and remove repeat targets, and the common targets of disease and drugs were obtained.The common targets were imported into STRING database and the protein-protein interaction (PPI) network was constructed.GO function and KEGG pathway enrichment analysis were performed for the common targets.【Result】 A total of 37 active components and 74 targets of Qiweng Huangbo powder were obtained, among which 68 common targets intersected with the disease.147 biological process, 15 cell component and 32 molecular function were obtained by GO functional annotation.98 pathways were obtained by KEGG pathway enrichment analysis.Quercetin, kaempferol, stigmasterol, tetrahyroberberine, berberine and so on were active components.Tumour necrosis factor (TNF), tumor protein p53(TP53), vascular endothelial growth factor A(VEGFA), interleukin 6 (IL6), IL10, chemokine 2(CCL2), epidermal growth factor (EGF), NF-kappa-B inhibitor alpha(NFKBIA) and so on were shown as key targets of the drug.Cancer, phosphatidylinositol-3/kinase protein kinase B(PI3K-Akt), mitogen-activated protein kinase (MAPK), IL17, inflammatory bowel disease and other signal pathways were the key pathways, and which also involved in immune response, transcription, apoptosis, cytokine activity, extracellular aspects.【Conclusion】 This study predicted the effective components and action mechanism of Qiweng Huangbo powder in the treatment of piglet diarrhea, which reflected the characteristics of multiple components, multiple targets and multiple pathways of Traditional Chinese medicine, and provided direction and reference for subsequent research.

Key words: network pharmacology; piglet diarrhea; Qiweng Huangbo powder; signal; pathways

中图分类号:

S853.74

陈文广, 张阳, 肖小玥, 刘艳艳, 陈雪英, 王秀敏, 秦俊杰, 李艳华. 基于网络药理学探究芪翁黄柏散治疗仔猪腹泻的有效成分及作用机制[J]. 中国畜牧兽医, 2022, 49(12): 4898-4908.

CHEN Wenguang, ZHANG Yang, XIAO Xiaoyue, LIU Yanyan, CHEN Xueying, WANG Xiumin, QIN Junjie, LI Yanhua. Investigation of the Effective Components and Active Mechanism of Qiweng Huangbo Powder in the Treatment of Piglet Diarrhea Based on Network Pharmacology[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4898-4908.

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