1株鹅源类志贺邻单胞菌的分离鉴定及致病性研究

doi:10.16431/j.cnki.1671-7236.2022.09.041

摘要/Abstract

摘要： 【目的】查明江苏某鹅场鹅发病及死亡原因。【方法】剖检病死鹅，运用细菌分离纯化、染色观察、生化试验、基质辅助激光解析电离飞行时间质谱（MALDI-TOF MS）鉴定、16S rDNA测定方法进行病原菌分离鉴定，通过药敏试验和小鼠致病性试验探究分离菌株的特性。【结果】从患病鹅肝脏分离到1株革兰氏阴性短杆状细菌；生化试验结果显示，此菌株可发酵葡萄糖、麦芽糖、枸橼酸盐、尿素；MALDI-TOF MS鉴定结果显示，此菌株为类志贺邻单胞菌；16S rDNA序列对比分析发现，分离菌与类志贺邻单胞菌相似性最高，达98%以上；药敏试验结果显示，分离菌对左氧氟沙星、美罗培南、头孢西丁、庆大霉素等10种抗菌药敏感，对四环素、头孢吡肟、头孢他啶、头孢唑林表现为中介，对阿奇霉素、复方新诺明、氨苄西林等6种药物表现为耐药，是典型的多重耐药菌；小鼠致病性试验结果显示，分离菌对小鼠的半数致死量为5.0×10^6.5 CFU。【结论】本试验首次报道了从鹅体内分离到致病性类志贺邻单胞菌，通过药敏试验筛选了左氧氟沙星、美罗培南、头孢西丁等有效的临床常用抗菌药，分离株对小鼠致病性较强，提示其带来的潜在风险不可忽视。

关键词: 类志贺邻单胞菌; 鹅; 药敏试验; 致病性

Abstract: 【Objective】 This study was aimed to find out the causes of morbidity and mortality of geese in a goose farm in Jiangsu province and reduce economic losses.【Method】 The dead geese were dissected and the pathogenic bacteria identified by bacterial isolation and purification, staining observation, biochemical test, MALDI-TOF MS identification and 16S rDNA determination.The characteristics of the isolated strains were explored by drug sensitivity test and mouse pathogenicity test.【Result】 A Gram-negative short rod-shaped bacterium was isolated from the liver of diseased geese.The results of biochemical tests showed that the strain could ferment glucose, maltose, citrate and urea.MALDI-TOF MS identification showed that the strain was Plesiomonas shigelloides.16S rDNA sequence analysis showed that the similarity between the isolated strain and Plesiomonas shigelloides was the highest, which was more than 98%.The results of drug sensitivity test showed that the isolated bacteria were sensitive to 10 kinds of antibiotics such as levofloxacin, meropenem, cefoxitin and gentamicin.The isolate was intermediary to tetracycline, cefepime, ceftazidime and cefazolin, and resistant to 6 kinds of antibiotics including azithromycin, compound sulfamethoxazole and ampicillin.The isolate was typical multidrug-resistant bacterium.The results of mouse pathogenicity test showed that the median lethal dose of the isolated bacteria to mice was 5.0×10^6.5 CFU.【Conclusion】 This experiment first reported the isolation of pathogenic Plesiomonas shigelloides from geese.Levofloxacin, meropenem, cefoxitin and other effective commonly used clinical antibiotics were screened through drug sensitivity test.The isolate was highly pathogenic to mice, suggesting that the potential risks brought by it should not be ignored.

Key words: Plesiomonas shigelloides; geese; antibiotic susceptibility test; pathogenicity

中图分类号:

S852.61⁺1

康宇晖, 周洪善, 金文杰, 秦爱建. 1株鹅源类志贺邻单胞菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2022, 49(9): 3676-3682.

KANG Yuhui, ZHOU Hongshan, JIN Wenjie, QIN Aijian. Isolation, Identification and Pathogenicity of a Strain of Plesiomonas shigelloides from Geese[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3676-3682.

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