不同浓度牛磺酸对猪精液负压条件常温保存效果的影响

doi:10.16431/j.cnki.1671-7236.2022.09.022

摘要/Abstract

摘要： 【目的】探讨负压条件下向Modena稀释剂中添加不同浓度牛磺酸对长白公猪精子常温保存质量及抗氧化能力的影响，以期为猪精液保存体系的完善提供参考。【方法】向Modena稀释剂中分别添加浓度为0（对照组）、1、5及10 mmol/L牛磺酸，各组精液在―0.04 Mpa条件下保存11 d。于第1、3、5、7、9及11天利用计算机辅助精子分析系统（CASA）检测精子活力、活率、畸形率、平均路径速度（VAP）、曲线速度（VCL）及鞭打频率（BCF）；利用试剂盒测定精液的总抗氧化能力（T-AOC）及H₂O₂含量。【结果】在保存1~11 d期间，3个牛磺酸组猪精子的活力及活率均显著高于对照组（P<0.05）；第11天时5 mmol/L牛磺酸组猪精子畸形率显著低于其他各组（P<0.05）；自第3天开始，1、5及10 mmol/L牛磺酸组猪精子的VAP均显著高于对照组（P<0.05），其中以5 mmol/L牛磺酸组效果最佳；第11天时，5 mmol/L牛磺酸组猪精子的VCL、BCF均显著优于其他各组（P<0.05）；牛磺酸可显著提升猪精子的T-AOC （P<0.05），但第11天时T-AOC较弱；第1~3天时，所有试验组间猪精子H₂O₂含量无显著差异，第5~11天时，3个牛磺酸组的H₂O₂含量均显著低于对照组（P<0.05）。【结论】在―0.04 Mpa条件下，向Modena稀释剂中添加牛磺酸可以显著改善常温保存猪精液的质量参数与抗氧化性能，其中以5 mmol/L添加量最好，保存时间在9 d以内为宜。

关键词: 负压; 牛磺酸; 猪精液; 常温保存

Abstract: 【Objective】 The aim of this study was to investigate the effect of adding different concentrations of taurine to Modena diluent under negative pressure on the quality and antioxidant capacity of Landrace boar sperm stored at room temperature, in order to provide reference for the improvement of pig semen preservation system.【Method】 Taurine was added to Modena diluent at the concentration of 0(control group), 1, 5 and 10 mmol/L, respectively, and the semen of each group were stored under the condition of-0.04 Mpa for 11 days.On 1st, 3rd, 5th, 7th, 9th and 11th days, sperm motility, viability, malformation rate, average path velocity (VAP), curvilinear velocity (VCL) and beatcross frequency (BCF) were detected by computer-aided sperm analysis system (CASA).The total antioxidant capacity (T-AOC) and H₂O₂ content of semen were determined by kits.【Result】 The motility and viability of boar sperm in the three taurine groups were significantly higher than those in control group during the storage period of 1-11 days (P<0.05).On the 11th day, the malformation rate of boar sperm in 5 mmol/L taurine group was significantly lower than that in other groups (P<0.05).From the 3rd day, the VAP of boar sperm in 1, 5 and 10 mmol/L taurine groups were significantly higher than those in control group (P<0.05), and the 5 mmol/L taurine group had the best effect.On the 11th day, the VCL and BCF of boar sperm in 5 mmol/L taurine group were significantly better than those in other groups (P<0.05).Taurine could significantly increase the T-AOC of boar sperm (P<0.05), but the T-AOC was almost undetectable on the 11th day.From 1 to 3 days, there was no significant difference in the H₂O₂ content of boar sperm among all test groups, from 5 to 11 days, the H₂O₂ content of the three taurine groups was significantly lower than that of the control group (P<0.05).【Conclusion】 Under the condition of -0.04 Mpa, adding taurine to Modena diluent could significantly improve the quality parameters and antioxidant properties of boar semen stored at room temperature, and the best concentration was 5 mmol/L, and the storage time was less than 9 days.

Key words: negative pressure; taurine; boar semen; room temperature preservation

中图分类号:

S814.3

王赫川, 张涵, 李天峰, 王骞, 张群, 郭铭晖, 孙超, 周彦芝, 李远, 李雁冰, 魏国生, 李井春. 不同浓度牛磺酸对猪精液负压条件常温保存效果的影响[J]. 中国畜牧兽医, 2022, 49(9): 3484-3491.

WANG Hechuan, ZHANG Han, LI Tianfeng, WANG Qian, ZHANG Qun, GUO Minghui, SUN Chao, ZHOU Yanzhi, LI Yuan, LI Yanbing, WEI Guosheng, LI Jingchun. Effects of Different Concentrations of Taurine on the Preservation of Boar Semen Under Negative Pressure at Room Temperature[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3484-3491.

参考文献

[1] BALIOU S, ADAMAKI M, IOANNOU P, et al.Protective role of taurine against oxidative stress[J].Molecular Medicine Reports, 2021, 24(2):605.
[2] JAKARIA M, AZAM S, HAQUE M E, et al.Taurine and its analogs in neurological disorders:Focus on therapeutic potential and molecular mechanisms[J].Redox Biology, 2019, 24(6):101223.
[3] SEOL S I, KIM H J, CHOI E B, et al.Taurine protects against postischemic brain injury via the antioxidant activity of taurine chloramine[J].Antioxidants, 2021, 10(3):372.
[4] ZHOU J, LU Y, WU W, et al.Taurine promotes the production of CD4⁺CD25⁺FOXP3⁺ Treg cells through regulating IL-35/STAT1 pathway in a mouse allergic rhinitis model[J].Allergy, Asthma & Clinical Immunology, 2021, 17(1):59.
[5] PATEL S N, PANDYA K, CLARK G J, et al.Comparison of taurine and pantoyltaurine as antioxidants in vitro and in the central nervous system of diabetic rats[J].Experimental and Toxicologic Pathology, 2016, 68(2-3):103-112.
[6] SURAI P F, EARLE P K, KIDD M T.Taurine as a natural antioxidant:From direct antioxidant effects to protective action in various toxicological models[J].Antioxidants, 2021, 10(12):1876.
[7] VASCO D, HEMANDEZ M, VAZQOEZ J M, et al.Generation of reactive oxygen species (ROS) by frozen-thawed boar spermatozoa[J].Reproduction in Domestic Animals, 2007, 42(2):89-90.
[8] 牛统娟.常温保存过程活性氧诱导猪精子凋亡的研究[D].杨凌:西北农林科技大学, 2021. NIU T J.Study on sperm apoptosis induced by reactive oxygen species during preservation at room temperature of boar semen[D].Yangling:Northwest A&F University, 2021.(in Chinese)
[9] LI J C, LI Q, LI Y B, et al.Effects of negative pressure applied before storage (liquid storage, 17℃) on boar semen quality and fertilization ability[J].Indian Journal of Animal Research, 2020, 54(10):1201-1205.
[10] 李琦, 李雁冰, 王硕, 等.不同负压条件对17℃保存猪精液生化指标的影响[J].中国畜牧杂志, 2018, 54(8):65-67. LI Q, LI Y B, WANG S, et al.Effect of different negative pressure conditions on biochemical parameters of boar semen stored at 17℃[J].Chinese Journal of Animal Science, 2018, 54(8):65-67.(in Chinese)
[11] REZAEE T F, ZEIDOONI L, RAFIEE Z, et al.Taurine effects on bisphenol A-induced oxidative stress in the mouse testicular mitochondria and sperm motility[J].JBRA Assisted Reproduction, 2020, 24(4):428-435.
[12] PERUMAL P, VUPRU K, RAJKHOWA C.Effect of addition of taurine on the liquid storage (5℃) of mithun (Bos frontalis) semen[J].Veterinary Medicine International, 2013, 2013(6):165348.
[13] ALY H A, KHAFAGY R M.Taurine reverses endosulfan-induced oxidative stress and apoptosis in adult rat testis[J].Food and Chemical Toxicology, 2014, 64(2):1-9.
[14] 张柳明.牛磺酸对湖羊精液常温保存效果的研究[D].扬州:扬州大学, 2021. ZHANG L M.Study on the effect of taurine on the preservation of Hu sheep semen at room temperature[D].Yangzhou:Yangzhou University, 2021.(in Chinese)
[15] 谢东淇, 刘琦, 苏泽智, 等.牛磺酸与抗生素复合剂对猪精液常温保存效果的影响[J].西北农业学报, 2017, 26(6):820-826. XIE D Q, LIU Q, SU Z Z, et al.Effect of taurine and antibiotics compounding agent on quality of boar semen stored at normal temperature[J].Acta Agriculturae Boreali-occidentalis Sinica, 2017, 26(6):820-826.(in Chinese)
[16] HIRANO Y, SHIBAHARA H, OBARA H, et al.Relationships between sperm motility characteristics assessed by the computer-aided sperm analysis (CASA) and fertilization rates in vitro[J].Journal of Assisted Reproduction and Genetics, 2001, 18(4):215-220.
[17] LIU D Y, CLARKE G N, BAKER H W.Relationship between sperm motility assessed with the Hamilton-Thorn motility analyzer and fertilization rates in vitro[J].Journal of Andrology, 1991, 12(4):231-239.
[18] SLANINA T, MIŠKEJE M, TIRPÁK F, et al.Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility[J].Czech Journal of Animal Science, 2018, 63(4):127-135.
[19] 陈宠霞, 金一.添加牛磺酸对兔精液液态保存质量参数的影响[J].畜牧与兽医, 2006, 8:25-27. CHEN C X, JIN Y.Effect of taurine addition on quality parameters of rabbit semen liquid preservation[J].Animal Husbandry & Veterinary Medicine, 2006, 8:25-27.(in Chinese)
[20] CANBAZ H, AKCA T, TATAROGLU C, et al.The effects of exogenous L-carnitine on lipid peroxidation and tissue damage in an experimental warm hepatic ischemia-reperfusion injury model[J].Current Therapeutic Research, 2007, 68(1):32-46.
[21] AITKEN R J, DREVET J R.The importance of oxidative stress in determining the functionality of mammalian spermatozoa:A two-edged sword[J].Antioxidants, 2020, 9(2):111.
[22] URIBE P, MERIÑO J, MATUS C E, et al.Autophagy is activated in human spermatozoa subjected to oxidative stress and its inhibition impairs sperm quality and promotes cell death[J].Human Reproduction, 2022, 37(4):680-695.
[23] JONG C J, SANDAL P, SCHAFFER S W.The role of taurine in mitochondria health:More than just an antioxidant[J].Molecules, 2021, 26(16):4913.
[24] TABASSUM H, REHMAN H, BANERJEE B D, et al.Attenuation of tamoxifen-induced hepatotoxicity by taurine in mice[J].Clinica Chimica Acta, 2006, 370(1-2):129-136.
[25] WANG Z, OHATA Y, WATANABE Y, et al.Taurine improves lipid metabolism and increases resistance to oxidative stress[J].Journal of Nutritional Science and Vitaminology, 2020, 66(4):347-356.
[26] LOU J, HAN D, YU H, et al.Cytoprotective effect of taurine against hydrogen peroxide-induced oxidative stress in UMR-106 cells through the Wnt/β-catenin signaling pathway[J].Biomolecules & Therapeutics, 2018, 26(6):584-590.
[27] BALAMURUGAN B, GHOSH S K, LONE S A, et al.Partial deoxygenation of extender improves sperm quality, reduces lipid peroxidation and reactive oxygen species during cryopreservation of buffalo (Bubalus bubalis) semen[J].Animal Reproduction Science, 2018, 189:60-68.
[28] 刘琦.不同精子质膜保护剂对猪精液常温保存效果的影响[D].杨凌:西北农林科技大学, 2015. LIU Q.Effects of different sperm plasma membrane protectant on boar semen stored at normal temperature[D].Yangling:Northwest A&F University, 2015.(in Chinese)