乌梅散提取物对兔离体小肠平滑肌运动的影响及机制研究

doi:10.16431/j.cnki.1671-7236.2021.02.009

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (2): 477-483.doi: 10.16431/j.cnki.1671-7236.2021.02.009

乌梅散提取物对兔离体小肠平滑肌运动的影响及机制研究

尚书凤, 王琦, 郭素芬, 陈志远, 王杨科

陕西理工大学生物科学与工程学院, 汉中 723000

收稿日期:2020-06-28 出版日期:2021-02-20 发布日期:2021-02-23
通讯作者: 王杨科 E-mail:497326701@qq.com
作者简介:尚书凤(1978-),女,陕西安康人,博士,研究方向:动物生理生化,E-mail:shangshf101291@163.com
基金资助:
陕西省科技厅青年人才项目（2016JQ3017）；陕西省教育厅专项科研计划项目（16JK1148）

Effects of the Dark Plum Powder Extraction on Contraction of Small Intestine Smooth Muscle of Rabbits in vitro and Its Mechanism

SHANG Shufeng, WANG Qi, GUO Sufen, CHEN Zhiyuan, WANG Yangke

College of Bioscience and Engineering, Shaanxi University of Technology, Hanzhong 723000, China

Received:2020-06-28 Online:2021-02-20 Published:2021-02-23

摘要/Abstract

摘要： 本研究旨在探究乌梅散提取物对兔离体小肠平滑肌收缩的作用及其机制。取乌梅30 g、柿蒂50 g、诃子12 g、黄连12 g、郁金12 g，通过煎熬制备乌梅散水提取物储液。兔小肠离体后恒温灌流，利用RM6240多道生物信号采集处理系统进行信息采集和记录。观察乌梅散提取物对离体小肠自发收缩的影响，应用乙酰胆碱（Ach）致使兔小肠平滑肌痉挛性收缩，观察中浓度乌梅散提取物（2.18 g/L）对Ach诱导的收缩的影响，并观察其对Ach引起的离体小肠细胞内Ca²⁺和胞外Ca²⁺收缩的影响。试验结果表明，在药物浓度大于1.09 g/L时，可显著抑制兔离体小肠平滑肌自发收缩的振幅和频率（P<0.05）；Ach可极显著诱导小肠平滑肌收缩的振幅（P<0.01）；2.18 g/L乌梅散提取物可极显著抑制Ach引起的痉挛性收缩振幅和频率（P<0.01）；在无Ca²⁺台氏液中，乌梅散提取物可极显著抑制Ach引起的离体小肠细胞内Ca²⁺和胞外Ca²⁺收缩振幅及频率（P<0.01）。综上所述，乌梅散提取物剂量依赖性地抑制小肠平滑肌自发收缩，可抑制小肠痉挛性收缩，其机制可能与抑制细胞内Ca²⁺释放和胞外Ca²⁺内流有关。

关键词: 乌梅散; 小肠平滑肌; Ca²⁺离子通道

Abstract: The effects of Dark Plum powder extraction on the contraction of small intestine in vitro were investigated and the possible mechanism was explored.The water extraction was prepared by decocting the following Chinese herbs including 30 g of Dark Plum,50 g of Calyx Kaki,12 g of Medicine Terminalia Fruit,12 g of Coptidis Rhizoma,and 12 g of Radix Curcumae.The technique of isothermal perfusion of small intestine in vitro was used.RM6240 multi-channel biological signal acquisition and processing system was used for information collection and processing.The effects of Dark Plum powder extraction on the spontaneous contraction of isolated gastrointestinal smooth muscles were observed.The effects of 2.18 g/L of Dark Plum powder extraction on the contraction induced by acetylcholine (Ach) were measured.The effects of 2.18 g/L of Dark Plum powder extraction on the Ach-induced both intracellular and extracellular calcium-depended contractions were studied.The amplitude and frequency of spontaneous contraction of isolated gastrointestinal smooth muscles were significantly reduced by Dark Plum powder extraction at the concentration of more than 1.09 g/L (P<0.05).Ach observably induced the amplitude of smooth muscle contractions (P<0.01).Dark Plum powder extraction at the concentration of 2.18 g/L markedly inhibited the amplitude and frequency of spasmodic contractions (P<0.01).In the Ca²⁺ free tyrodes solution,Dark Plum powder extraction evidently inhibited the amplitude and frequency of the Ach-induced both intracellular and extracellular calcium-depended contractions (P<0.01).Dark Plum powder extraction inhibited the spontaneous contraction and spasmodic contraction caused by Ach in a dose-dependent manner,isolated gastric smooth muscle.The mechanism was speculated to be related to inhibiting the extracellular Ca²⁺ inflowing via cell membrane and intracellular Ca²⁺ releasing.

Key words: Dark Plum powder; gastrointestinal smooth muscle; calcium channel

中图分类号:

S829.1

尚书凤, 王琦, 郭素芬, 陈志远, 王杨科. 乌梅散提取物对兔离体小肠平滑肌运动的影响及机制研究[J]. 中国畜牧兽医, 2021, 48(2): 477-483.

SHANG Shufeng, WANG Qi, GUO Sufen, CHEN Zhiyuan, WANG Yangke. Effects of the Dark Plum Powder Extraction on Contraction of Small Intestine Smooth Muscle of Rabbits in vitro and Its Mechanism[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 477-483.

参考文献

[1] 胡元亮.中兽医学[M].北京:科学出版社,2013. HU Y L.Chinese Veterinary Medicine[M].Beijing:Science Press,2013.(in Chinese)
[2] 张华月,李琦,付晓伶.乌梅化学成分及药理作用研究进展[J].上海中医药杂志,2017,51(S1):296-300. ZHANG H Y,LI Q,FU X L,et al.Research progress on chemical components and pharmacological effects of Wumei[J].Shanghai Journal of Traditional Chinese Medicine,2017,51(S1):296-300.(in Chinese)
[3] 张小琼,侯晓军,杨敏,等.乌梅的药理作用研究进展[J].中国药房,2016,27(25):3567-3570. ZHANG X Q,HOU X J,YANG M,et al.Research progress of the pharmacological action of Mume[J].China Pharmacy,2016,27(25):3567-3570.(in Chinese)
[4] GROSSMAN L D,ROSCOE R,SHACK A R.Complementary and alternative medicine for diabetes[J].Canadian Journal of Diabetes,2018,42:S154-S161.
[5] YANG X P,LI L L,FANG K,et al.Wu-Mei-Wan reduces insulin resistance via inhibition of NLRP3 inflammasome activation in HepG2 cells[J].Evidence-based Complementray and Alternative Medicine,2017,2017:7283241.
[6] ZHANG X J,HE L J,LU Q,et al.Pharmacological activity of Terminalia chebula[J].China Journal of Chinese Materia Medica,2016,41(4):619-623.
[7] 王军,张秀凤,宋予震,等.诃子粗提物影响家兔离体小肠运动的试验[J].中国兽医杂志,2010,6(1):17-19. WANG J,ZHANG X F,SONG Y Z,et al.Influence of Terminalia chebula extracts on the small bowel movement of rabbits[J].Chinese Journal of Veterinary Medicine,2010,6(1):17-19.(in Chinese)
[8] JUNG H L,YANG S Y,MIN C P,et al.Protective effects of chebulic acid from Terminalia chebula Retz.against t-BHP-induced oxidative stress by modulations of Nrf2 and its related enzymes in HepG2 cells[J].Food Science and Biotechnology,2019,28(2):555-562.
[9] YU J H,JIN M L,CHOI Y A,et al.Suppressive effect of an aqueous extract of Diospyros kaki calyx on dust mite extract/2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions[J].International Journal of Molecular Medicine,2017,40(2):505-511.
[10] PARK S B,PARK G H,SONG H M,et al.Anticancer activity of calyx of Diospyros kaki Thunb.through downregulation of cyclin D1 via inducing proteasomal degradation and transcriptional inhibition in human colorectal cancer cells[J].BMC Comple-mentary & Alternative Medicine,2017,17(1):445.
[11] 许家玉.乌梅散加减方治疗猪传染性胃肠炎效果研究[J].安徽农学通报,2016,22(8):107-110. XU J Y.Effect of porcine transmissible gastroenteritis treatment of Plum powder[J].Anhui Agronomy Bulletin,2016,22(8):107-110.(in Chinese)
[12] 张登鹤,马正文.乌梅散加味治疗仔猪红黄白痢[J].中兽医医学杂志,2017,1:28. ZHANG D H,MA Z W.Modified Wumei powder in the treatment of red,yellow and white diarrhea of piglets[J].Chinese Journal of Traditional Veterinary Science, 2017,1:28.(in Chinese)
[13] 宋海德.乌梅散治疗运输应激引起的犊牛腹泻[J].青海畜牧兽医杂志,2011,41(5):28. SONG H D.Wumei powder in the treatment of calf diarrhea caused by transport stress[J].Chinese Qinghai Journal of Animal and Veterinary Sciences,2011,41(5):28.(in Chinese)
[14] 刘艳艳,李明珠,张善婷,等.乌梅散加味辅助治疗犬球虫病[J].黑龙江畜牧兽医,2012,20:102-103. LIU Y Y,LI M Z,ZHANG S T,et al.Auxiliary treatment of canine Coccidiosis with modified Wumei powder[J].Heilongjiang Animal Science and Veterinary Medicine,2012,20:102-103.(in Chinese)
[15] 张刚,陆启航.Cajal间质细胞相关分子调控及临床疾病的研究进展[J].外科,2019,8(2):51-58. ZHANG G,LU Q H.Research progress of molecular regulation and clinical diseases Cajal related to stromal cell[J].Surgery,2019,8(2):51-58.(in Chinese)
[16] 李毅,齐清会.胃肠道神经-Cajal间质细胞-平滑肌网络研究进展[J].国际消化病杂志,2007,27(3):225-227. LI Y,QI Q H.Study progress of the gastrointestinal nerve-ICC-smooth muscle network[J].International Journal of Digestive Disease,2007,27(3):225-227.(in Chinese)
[17] 纪云西,凌江红,庞黎明,等.胃肠道Cajal间质细胞、Ca²⁺与胃肠动力[J].实用医学杂志,2013,29(11):1872-1873. JI Y X,LING J H,PANG L M,et al.Interstitial Cajal cells,Ca²⁺ and gastrointestinal motility[J].The Journal of Practical Medicine,2013,29(11):1872-1873.(in Chinese)
[18] 常宗宏,邓尚新,杨娟,等.Cajal间质细胞在胃肠动力障碍性疾病中的研究[J].胃肠病学和肝病学杂志,2019,28(4):387-390. CHANG Z H,DENG S X,YANG J,et al.The research of interstitial cells of Cajal in disorders of gastrointestinal motility[J].Chinese Journal of Gastroen-terology and Hepatology,2019,28(4):387-390.(in Chinese)
[19] 陈杰,蔡威.小肠平滑肌功能紊乱机制研究进展[J].临床儿科学,2012,30(2):195-197. CHEN J,CAI W.Research progress on intestinal smooth muscle dysfunction[J].Journal of Clinical Pediatrics,2012,30(2):195-197.(in Chinese)
[20] DRUMM B T,HENNING G W,BATTERSBY M J,et al.Clustering of Ca²⁺ transients in interstitial cells of Cajal defines slow wave duration[J].Journal of General Physiology,2017,149(7):703-725.
[21] MONTGOMERY L E,TANSEY E A,JOHNSON C D,et al.Autonomic modification of intestinal smooth muscle contractility[J].Advances Physiology Education,2016,40(1):104-109.
[22] MATSUI M,MOTOMURA D,FUJIKAWA T,et al.Mice lacking M₂ and M₃ muscarinic acetylcholine receptors are devoid of cholinergic smooth muscle contractions but still viable[J].The Journal of Neuroscience,2002,22(24):10627-10632.
[23] CHASEN J G,ROBERT A C.Muscarinic acetylcholine receptors participate in small intestinal mucosal homeostasis[J].Journal of Pediatric Surgery,2017,52(3):1031-1034.
[24] SAKAMOTO T,UNNO T,KITAZAWA T,et al.Three distinct muscarinic signalling pathways for cationic channel activation in mouse gut smooth muscle cells[J].Journal of Physiology,2007,582(1):41-61.

乌梅散提取物对兔离体小肠平滑肌运动的影响及机制研究

Effects of the Dark Plum Powder Extraction on Contraction of Small Intestine Smooth Muscle of Rabbits in vitro and Its Mechanism

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