捻转血矛线虫虫卵的大量分离纯化

›› 2008, Vol. 1 ›› Issue (12): 120-122.

捻转血矛线虫虫卵的大量分离纯化

荣光^1，2，赵军明¹，郑金海¹，王新华¹，薄新文¹，钟发刚¹，罗盘琪¹，任耀军¹

1.新疆兵团绵羊繁育生物技术重点实验室，石河子 832003； 2.中国热带农业科学院品资所，儋州 571737

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-12-20 发布日期:2008-12-20

Separation and Purification of Much Eggs from Haemonchus Contorts

RONG Guang^1,2, ZHAO Junming¹, ZHENG Jinhai¹, WANG Xinhua^1, BO Xinwen¹, ZHONG Fagang¹, LUO Panqi¹, REN Yaojun¹

1.The Key Sheepbreeding Laboratory of Xinjiang Production and Construction Group,Shihezi 832003，China; 2. Tropical Crop Genetic Resources Institute,CATAS, Danzhou 571737,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

摘要/Abstract

摘要： 笔者采用虫体取卵和孵化后感染性幼虫直接在培养瓶瓶盖内收集的方法，获得了大量纯净单一的感染性幼虫，并且感染绵羊成功。再对感染绵羊直肠采粪收集虫卵，就可收集到大量纯种单一虫卵，进行三期幼虫的大量孵化。该试验解决了在寄生性线虫分子生物学研究中需要大量单一虫种的纯化分离问题。

关键词: 捻转血矛线虫; 虫卵; 分离纯化

Abstract: By gathering eggs from female adults of H. contortus,and hatching their eggs in incubation bottles,lots of monospecifical infectious larvae on the lids of these bottles were obtained, and contanmination was avoided from other worms.It was successful with these larvae infencting sheep. Then a lot of purebred L3 stage larvaes from the haemonchus contorts eggs were collected and got by salt floatation in the faeces of monospecifically infected sheep.The method provides a way to get purebred larvae in molecular biology research of parasites.

Key words: haemonchus contorts; eggs; separation and purification

荣光;赵军明;郑金海;王新华;薄新文;钟发刚;罗盘琪;任耀军. 捻转血矛线虫虫卵的大量分离纯化[J]. , 2008, 1(12): 120-122.

RONG Guang;ZHAO Junming;ZHENG Jinhai;WANG Xinhua;BO Xinwen;ZHONG Fagang;LUO Panqi;REN Yaojun. Separation and Purification of Much Eggs from Haemonchus Contorts[J]. China Animal Husbandry & Veterinary Medicine, 2008, 1(12): 120-122.

[1]	吴正姣, 侯林静, 饶国顺, 靳纬坤, 方芳, 张为宇, 邸文达. 广西南宁市水牛肝脏内前后盘吸虫的鉴定[J]. 中国畜牧兽医, 2021, 48(2): 668-675.
[2]	陆宝燕, 张艳艳, 马勋, 王云菲, 王正荣, 孟季蒙, 蒋建军, 薄新文. 苦楝提取物对捻转血矛线虫卵和幼虫活性的影响[J]. 中国畜牧兽医, 2020, 47(8): 2579-2587.
[3]	韩利方, 闫文朝, 李亚云, 杜婷婷, 钱伟锋, 张旻, 位治国, 王天奇. 球虫消毒剂的筛选及氨水对艾美耳球虫卵囊抑杀条件的优化[J]. 《中国畜牧兽医》, 2018, 45(12): 3601-3607.
[4]	王玺德;盛书娟;祝建平;崔希云;谢德颖. 石榴皮提取物对离体培养兔球虫卵囊的杀灭作用[J]. , 2012, 39(2): 212-215.
[5]	张继才;付美芬;杨世平;王安奎;黄必志;袁希平;杨国荣. 不同驱虫药及组合对BMY断奶犊牛体内寄生虫的驱除效果研究[J]. , 2009, 36(2): 114-115.