›› 2007, Vol. 34 ›› Issue (9): 47-49.

• 生物技术 • 上一篇    下一篇

山羊催乳素受体基因部分片段的克隆与序列分析

储明星1，张跟喜2，刘荣志3，王金玉2，方丽1，叶素成1,刘雪云4，马明德4
   

  1. 1.中国农业科学院北京畜牧兽医研究所，北京 100094；2.扬州大学动物科技学院，扬州 225009；3 中国农学会，北京 100026；4.山东省嘉祥县畜牧局，嘉祥 272400
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-20 发布日期:2007-09-20

Cloning and Sequence Analysis of Partial Fragment of Prolactin Receptor Gene in Goats

CHU Mingxing1, ZHANG Genxi2, LIU Rongzhi3, WANG Jinyu2, FANG Li1, YE Sucheng1, LIU Xueyun4,MA Mingde4   

  1. 1.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China； 2.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 3.China Association of Agricultrual Science Societies,Beijing 100026,China; 4.Animal Husbandry Bureau of Jiaxiang County in Shandong Province,Jiaxiang 272400,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20

摘要： 采用PCR技术扩增出济宁青山羊催乳素受体基因长892 bp的片段，该片段含有97 bp的部分外显子8序列（外显子8全长为100 bp）、683 bp的内含子8、70 bp的外显子9及42 bp的部分内含子9。将该片段克隆到pGEM-T Easy质粒中，重组质粒用PCR进行阳性克隆鉴定，然后测定核苷酸序列，并推导其氨基酸序列。该序列与绵羊、母牛、人、大鼠、小鼠的催乳素受体基因mRNA的对应序列的核苷酸同源性分别为99.4 %、97.01%、89.22%、89.22%、88.02%，氨基酸同源性分别为100%、94.55%、81.88%、81.82%、83.64%。

关键词: 山羊; 催乳素受体基因; 克隆; 序列分析

Abstract: The 892 bp fragment （97 bp of part of exon 8 (the full length of exon 8 was 100 bp), 683 bp of intron 8, 70 bp of exon 9 and 42 bp of part of intron 9）of prolactin receptor (PRLR) gene was amplified successfully in Jining Grey goats by PCR and cloned into pGEM-T Easy vector. The positive clones were further identified by PCR analysis. The nucleotide sequence was detected and the peptide sequence of this fragment was deduced. This sequence shared 99.4 %, 97.01%, 89.22%, 89.22%, 88.02% nucleotide homology with the published mRNA of PRLR gene of sheep, cow, human, rat and mouse separately, and the amino acid homology was 100%, 94.55%, 81.88%, 81.82%, 83.64% separately.

Key words: goat; prolactin receptor gene; cloning; sequence analysis

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