氟喹诺酮类药物的多残留酶免疫分析研究

›› 2007, Vol. 34 ›› Issue (11): 126-129.

氟喹诺酮类药物的多残留酶免疫分析研究

潘孝成1，祁克宗2，朱良强3，赵瑞宏1

1.安徽省农科院畜牧兽医研究所，合肥 230031；2.安徽农业大学，合肥 230036；3.安徽省兽医站,合肥 230022

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-11-20 发布日期:2007-11-20

Study on the Multi Residues of Fluoroquinolones by Enzyme Immunoassay

PAN Xiaocheng1, QI Kezong2,ZHU Liangqiang3,ZHAO Ruihong1

1.Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China;2.Anhui Agricultural University, Hefei 230036, China; 3. Anhui Veterinary Station, Hefei 230022, China 

Received:1900-01-01 Revised:1900-01-01 Online:2007-11-20 Published:2007-11-20

摘要/Abstract

摘要： 以制备的多抗血清为基础，建立了氧氟沙星（OFL）、二氟沙星（DIF）和诺氟沙星（NOR）的多残留间接竞争酶免疫吸附测定法(CiELISA法)。OFL、 DIF和NOR的标准曲线的线性回归方程分别为y=-0.1984x+0.9687 (r=0.9885)、y=-0.1738x+0.9150 (r=0.9985)、y=-0.2004x+0.9572(r=0.9954)；中值（IC50）分别为231.57、245.32 、186.14 ng/ml ；最低检测限（LOD）分别为1.62、1.86、1.07 ng/ml；标准曲线在2~10000 ng/ml之间均有良好的线性关系(r≥0.9885)。方法的批内CV≤5.44%,批间CV≤12.45%。当OFL、DIF或NOR以浓度15~10000 ng/ml在牛奶中添加时，药物能较好地被回收，回收率均在71.70%~94.53%之间。

关键词: 氟喹诺酮类药物; 多残留; 间接竞争ELISA法

Abstract: The antiserum was prepared with the mixture of OFL-BSA,DIF-BSA and NORBSA.The Ci-ELISA method for the detection of ofloxacin ,difloxacin and norfloxacin was established with it.To ofloxacin ,difloxacin and norfloxacin.The regression equations about their standard curves were y=-0.1984x+0.9687(r=0.9885),y=-0.1738x+0.9150 (r=0.9985) and y=-0.2004x+0.9572 (r=0.9954),respectively.The 50% inhibition concentration(IC50) were 231.57,245.32 and 186.14ng/ml,respectively. The limits of detect (LOD) were 1.62,1.86 and 1.07ng/ml ,respectively. The coefficients of variation for the Ci-ELISA method were less than 5.44% in intra-assay and 12.45% in inter-assay. The recoveries in milk ranged from 71.70% to 94.53%.

中图分类号:

S859.84

潘孝成;祁克宗;朱良强;赵瑞宏. 氟喹诺酮类药物的多残留酶免疫分析研究[J]. , 2007, 34(11): 126-129.

PAN Xiaocheng;QI Kezong;ZHU Liangqiang;ZHAO Ruihong. Study on the Multi Residues of Fluoroquinolones by Enzyme Immunoassay [J]. , 2007, 34(11): 126-129.

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