›› 2011, Vol. 38 ›› Issue (6): 34-37.

• 动物营养与饲料科学 • 上一篇    下一篇

体外法研究不同磷添加水平对奶牛瘤胃内pH 和挥发性脂肪酸的影响

初汉平   

  1. 菏泽学院动物科学系,山东菏泽 274000
  • 收稿日期:2010-12-06 修回日期:1900-01-01 出版日期:2011-06-20 发布日期:2011-06-20

Effects of Different Inorganic Phosphorus Adding Levels on pH and Volatile Fatty Acid in Dairy Cows in vitro

CHU Han-ping   

  1. College of Animal Science and Technology,Heze University,Heze 274000,China
  • Received:2010-12-06 Revised:1900-01-01 Online:2011-06-20 Published:2011-06-20

摘要: 采用体外法研究无机磷添加浓度对瘤胃发酵的影响。无机磷在培养液中设0、30、60、90、120、150、200、250、300、350 mg/L 10个水平。体外培养发酵48 h后终止培养,测定培养液的pH、总挥发性脂肪酸(VFA)。结果表明,不同无机磷添加水平对乙酸、丙酸、丁酸和总VFA产量影响显著(P<0.05),无机磷添加水平为200 mg/L时,乙酸、丙酸、丁酸及总VFA产量最高,随后下降。瘤胃培养液pH随无机磷添加水平的增加而显著降低(P<0.05),无机磷添加水平为200 mg/L时达到最小值5.65,随后又上升。在本试验条件下,添加无机磷明显促进瘤胃微生物对饲料养分的发酵,适宜添加水平为200 mg/L。

关键词: 体外法; 无机磷; pH; 挥发性脂肪

Abstract: The effects of rumen fermentation by adding inorganic phosphorus in vitro were studied,Concentration of inorganic phosphorus were 0,30,60,90,120,150,200,250,300 and 350 mg/L. The pH and volatile fatty acid (VFA) of 48 h fermentation in vitro were measured. The results as follows: Acetate,propionate,butyrate acid and total VFA production were significantly effected by the concentration of inorganic phosphorus(P<0.05). Acetate,propionate,butyrate acid and total VFA production were the highest when the concentration of inorganic phosphorus was 200 mg/L. pH was significantly influenced by different inorganic phosphorus levels(P<0.05). pH decreased when inorganic P levels increased(P<0.05)and reached its minimum when inorganic phosphorus was 200 mg/L.In this experiment the optimum adding levels of inorganic phosphorus was 200 mg/L.

Key words: in vitro; inorganic phosphorus; pH; volatile fatty acid (VFA)

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