新疆家蚕抗菌肽可有效抑制掌面革兰氏阴性菌的研究

中国畜牧兽医 ›› 2014, Vol. 41 ›› Issue (9): 239-244.

新疆家蚕抗菌肽可有效抑制掌面革兰氏阴性菌的研究

康苏, 夏丽洁, 刘军, 马纪, 张富春

新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 新疆乌鲁木齐 830046

收稿日期:2014-03-28 出版日期:2014-09-20 发布日期:2014-09-24
通讯作者: 张富春 E-mail:zfcxju@gmail.com
作者简介:康苏（1987- ），女，甘肃人，硕士生，研究方向：生物化学与分子生物学。
基金资助:
新疆维吾尔自治区高技术研究发展计划项目（201110101）。

Research on CecropinXJ Effectively Inhibited the Palm Gram-negative Bacteria

KANG Su, XIA Li-jie, LIU Jun, MA Ji, ZHANG Fu-chun

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China

Received:2014-03-28 Online:2014-09-20 Published:2014-09-24

摘要/Abstract

摘要： 抗菌肽具有抑制细菌的作用，是天然防腐剂和潜在的抗生素替代品。为了解新疆家蚕抗菌肽（CecropinXJ）能否有效抑制手掌表面细菌，本研究采用擦拭法获取手掌表面细菌擦拭液，并用不同浓度CecropinXJ处理手掌擦拭液，通过平板菌落计数法评价CecropinXJ的抑菌效果，结果表明随着CecropinXJ浓度增加，其抑菌能力越好。当CecropinXJ添加到0.4%时，抑菌能力达到商业洗手液水平（P>0.05）；进一步对16S rDNA进行测序分析，结果发现手掌表面细菌多为革兰氏阴性菌，添加CecropinXJ后，手掌表面的革兰氏阴性菌生长能有效被抑制。本试验结果表明CecropinXJ可以用作消毒清洁产品进行开发。

关键词: 新疆家蚕抗菌肽; 手掌表面细菌; 平板菌落计数; 革兰氏阴性菌

Abstract: Antimicrobial peptide (AMP) could kill bacteria, which had been used as a natural preservative and potential alternative to antibiotics. To understand whether the CecropinXJ could effectively inhibit bacteria from palm skin, we used sterile water to swab hands and treated with swabbed liquor by different concentrations of CecropinXJ in this study. The antimicrobial activity was evaluated by plate count method. The results showed that the growth of bacteria derived from palm skin could be effectively inhibited via adding different concentrations of CecropinXJ, and the antimicrobial activity became stronger with increasing the concentrations of CecropinXJ. When the concentration of CecropinXJ was up to 0.4%, its inhibitory capacity could be comparable to commercial hand sanitizer (P>0.05). Further study elucidated that swabbed liquor included most of gram-negative bacteria through 16S rDNA sequence analysis. These findings suggested that CecropinXJ could be used as a novel disinfector or cleanser for application potentially.

Key words: CecropinXJ; palm surface bacteria; plate count; gram-negative bacteria

中图分类号:

S852.61

康苏, 夏丽洁, 刘军, 马纪, 张富春. 新疆家蚕抗菌肽可有效抑制掌面革兰氏阴性菌的研究[J]. 中国畜牧兽医, 2014, 41(9): 239-244.

KANG Su, XIA Li-jie, LIU Jun, MA Ji, ZHANG Fu-chun. Research on CecropinXJ Effectively Inhibited the Palm Gram-negative Bacteria[J]. , 2014, 41(9): 239-244.

参考文献

1 刘忠渊, 张富春, 蔡伦, 等. 酵母菌中表达的新疆家蚕抗菌肽(Cecropin-XJ)的特性研究[J]. 微生物学报, 2003, 43(5): 635～640.

2 刘宏道, 计融, 付平, 等. GB/T 4789.2—2003 食品卫生微生物学检验: 菌落总数测定[S]. 北京: 中国标准出版社, 2003.

3 许飞, 戴欣, 陈月琴, 等. 南沙海区沉积物中细菌和古细菌 16S rDNA多样性的研究[J]. 海洋与湖沼, 2004, 35(1): 89～94.

4 伍小松, 罗振福, 李俊波, 等. 天蚕素类抗菌肽的抗菌性能测定及其性质研究[J]. 中国畜牧兽医, 2009, 36(2): 28～32.

5 李启, 吴建明, 许乐, 等. 高危科室消毒灭菌质量监测评价的研究[J]. 中华医院感染学杂志, 1999, 9(2): 107.

6 李秋剑. 哺乳动物抗菌肽的研究进展[J]. 科技与实践, 2006, 42(20): 60～62.

7 吴爱平, 吴学贵, 章勇. 医院消毒方法的评价与思考[J]. 中华医院感染学杂志, 2000, 10(2): 123.

8 吴静, 梁永利, 李玉峰, 等. 重组鸡 Cathelicidins 类抗菌肽的融合表达及其对金黄色葡萄球菌抗菌活性的研究[J]. 中国畜牧兽医, 2013, 40(6): 19～26.

9 郑力, 李友广. 新疆家蚕抗菌肽在番茄汁加工中的应用研究[J].食品科学, 2007, 28(3): 135～139.

10 康苏, 夏丽洁, 马纪.新疆家蚕抗菌肽对小鼠体征指标和脏器组织影响的研究[J].食品工业科技, 2013, 34(21): 343～347.

11 Boman H G. Antibacterial peptides: Key components needed in immunity[J]. Cell, 1991, 65: 205～207.

12 Delves-Broughton J. Nisin as a food preservative[J]. Food Australia, 2005, 57(12): 525～527.

13 Fadaei V. Milk proteins-derived antibacterial peptides as novel functional food ingredients[J]. Ann Biol Res, 2012, 3(5): 2520～2526.

14 Fuller N J, Marie D, Partensky F, et al. Clade-specific 16S ribosomal DNA oligonucleotides reveal the predominance of a single marine Synechococcus clade throughout a stratified water column in the red sea[J]. Applied and Environmental Microbiology, 2003, 69(5): 2430～2443.

15 Gálvez A, Maqueda M, Martínez-Bueno M, et al. Permeation of bacterial cells, permeation of cytoplasmic and artificial membrane vesicles, and channel formation on lipid bilayers by peptide antibiotic AS-48[J].J Bacteriol, 1991, 173(2): 886～892.

16 Grande M J, Lucas R, Abriouel H, et al. Control of Alicyclobacillus acidoterrestris in fruit juices by enterocin AS-48[J]. International Journal of Food Microbiology, 2005, 104(3): 289～297.

17 Grande M J, Abriouel H, Lucas López R.Efficacy of enterocin AS-48 against bacilli in ready-to-eat vegetable soups and purees[J].J Food Prot, 2007, 70(10): 2339～2345.

18 Hao Y Q, Zhou X D, Xiao X R, et al. Effects of cecropin-XJ on growth and adherence of oral cariogenic bacteria in vitro[J]. Chin Med J (Engl), 2005, 118: 155～160.

19 Hugenholtz P, Huber T. Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases[J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53(1): 289～293.

20 Toke O. Antimicrobial peptides: New candidates in the fight against bacterial infections[J]. Peptide Science, 2005, 80(6): 717～735.

21 Venkitanarayanan K S, Zhao T, Doyle M P. Antibacterial effect of lactoferricin B on Escherichia coli O157：H7 in ground beef[J]. Journal of Food Protection, 1999, 62(7): 747～750.

22 Xia L, Zhang F, Liu Z, et al. Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli[J]. Experimental and Therapeutic Medicine, 2013a, 5(6): 1745～1751.

23 Xia L, Liu Z, Ma J, et al. Expression, purification and characterization of cecropin antibacterial peptide from Bombyx mori in Saccharomyces cerevisiae[J]. Protein Expression and Purification, 2013b, 90(1): 47～54.

24 Zasloff M. Inducing endogenous antimicrobial peptides to battle infections[J]. Proceedings of the National Academy of Sciences, 2006, 103(24): 8913～8914.

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