新疆南疆微小牛蜱Bm86基因及其编码蛋白剖析

中国畜牧兽医

新疆南疆微小牛蜱Bm86基因及其编码蛋白剖析

刘永宏，赵丽

塔里木大学动物科学学院，新疆生产建设兵团塔里木畜牧科技重点实验室，新疆阿拉尔 843300

收稿日期:2014-02-24 出版日期:2014-08-20 发布日期:2014-08-22
通讯作者: 赵丽。
作者简介:刘永宏（1981—），男，内蒙古人，博士，副教授，研究方向：动物传染病与免疫病理学。
基金资助:
新疆生产建设兵团博士资金项目（2012BB023）；大学生创新创业训练计划项目（2013107570030）。

Analysis of Bm86 Gene and its Encoding Protein of Rhipicephalus microplus in South Xinjiang

LIU Yong-hong, ZHAO Li

Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production & Construction Corps, College of Animal Science, Tarim University, Alar 843300, China

Received:2014-02-24 Online:2014-08-20 Published:2014-08-22

摘要/Abstract

摘要： 为了能高效表达理想的微小牛蜱抗原，本研究对新疆生产建设兵团塔里木畜牧科技重点实验室已获得的Bm86基因（Bm86 Tarim）进行遗传进化分析，并对其编码蛋白进行生物学信息分析。结果显示本研究获得的新疆南疆微小牛蜱Bm86 Tarim序列与已登录的所有国外Bm86基因完整阅读框序列的核苷酸和氨基酸同源性分别为95.9%～98.6%和93.8%～97.8%，差异极小；遗传进化树分析结果显示本研究毒株与已登录多数毒株在同一分支内；生物学信息预测结果显示该蛋白不稳定，不溶性的概率为93.26%，有4个潜在糖基化位点。本试验结果为后续Bm86基因表达、免疫抗原制备和抗微小牛蜱疫苗的研制奠定基础。

关键词: 微小牛蜱; Bm86基因; Bm86蛋白

Abstract: In order to highly express the ideal Rhipicephalus microplus antigen, we analyzed genetic evolution of Bm86 gene on the laboratory acquired in this study and biological information of its coding protein. The results showed that the homologies of complete open reading frame sequences of nucleotide and amino acid sequence of Bm86 gene of Rhipicephalus microplus with all the foreign Bm86 gene logged in GenBank were 95.9% to 98.6% and 93.8% to 97.8%，respectively. The difference was very small. The evolutionary tree showed that the strain and most of logged strains were in same branch. Biology information prediction showed that the protein was unstable. Insoluble probability was 93.26%. There were 4 potential glycosylation sites. Analysis of the above information would lay the foundation for the gene expression, preparation of immune antigen and development of vaccine against Rhipicephalus microplus.

Key words: Rhipicephalus microplus; Bm86 gene; Bm86 protein

刘永宏，赵丽. 新疆南疆微小牛蜱Bm86基因及其编码蛋白剖析[J]. 中国畜牧兽医.

LIU Yong-hong, ZHAO Li. Analysis of Bm86 Gene and its Encoding Protein of Rhipicephalus microplus in South Xinjiang[J]. .

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