冷冻方法对杜泊绵羊冷冻精液品质的影响

中国畜牧兽医

冷冻方法对杜泊绵羊冷冻精液品质的影响

王新奇¹^，2，苏和¹，达来¹，杨燕燕¹，曹贵方²

1.内蒙古自治区农牧业科学院畜牧研究所，内蒙古呼和浩特 010031；2.内蒙古农业大学兽医学院，内蒙古呼和浩特 010018

收稿日期:2013-12-25 出版日期:2014-08-20 发布日期:2014-08-22
通讯作者: 杨燕燕（1980—），女，内蒙古人，博士，研究方向:动物遗传育种。Tel：0471-5296076；E-mail：swallow_0088@163.com 曹贵方（1963—），男，内蒙古人，博士生导师，研究方向：动物组织胚胎与发育生物学。Tel：0471-4303140；E-mail：guifangcao@126.com
作者简介:王新奇（1988—），男，山东人，硕士，研究方向：绵羊精液冷冻保存。
基金资助:
内蒙古肉羊标准化繁育关键技术研究与示范（2130106）。

Effects of Freezing Methods for Dorper Sheep Frozen Semen Quality

WANG Xin-qi¹^，2, SU He¹, DA Lai¹, YANG Yan-yan¹, CAO Gui-fang²

1.Institute of Agriculture and Animal Husbandry in Inner Mongolia Academy of Animal Husbandry, Hohhot 010031, China；2. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2013-12-25 Online:2014-08-20 Published:2014-08-22

摘要/Abstract

摘要： 试验对绵羊精液分别采用液氮熏蒸冷冻法和冷冻仪冷冻法进行冷冻，通过测定精液解冻后精子活率、顶体完整率、质膜完整率和谷草转氨酶活性及乳酸脱氢酶活性来比较不同的冷冻方法对解冻后精液品质的影响；通过测定精液稀释后、平衡后、冷冻仪法冷冻后的酶活性来比较谷草转氨酶与乳酸脱氢酶在不同阶段的释放量。结果表明，采用冷冻仪法冷冻后的精子活率和质膜完整率极显著高于液氮熏蒸冷冻法（P<0.01）；冷冻仪法冷冻后的谷草转氨酶和乳酸脱氢酶活性极显著低于液氮熏蒸冷冻法（P<0.01）；精液稀释后的谷草转氨酶和乳酸脱氢酶活性极显著低于精液冷冻后的活性（P<0.01）。表明采用冷冻仪冷冻法的绵羊精液品质好于液氮熏蒸冷冻法。精子中谷草转氨酶主要在平衡阶段释放，而乳酸脱氢酶主要在冷冻阶段释放。

Abstract: The sheep semen were freezing by liquid nitrogen vapour method and apparatus freezing frozen method respectively, measuring thawed semen by sperm motility, acrosome integrity, membrane integrity and aspartate aminotransferase and lactate dehydrogenase activity to compare effect of different methods of freezing semen quality after thawing；measuring enzyme activity after semen dilution, equilibration, apparatus freezing frozen method to compare the release amount of aspartate aminotransferase and lactate dehydrogenase at different stages. The results showed that the sperm motility and membrane integrity after apparatus freezing frozen method were highly significant higher than the liquid nitrogen fumigation freezing method (P<0.01)；aspartate aminotransferase and lactate dehydrogenase activity after apparatus freezing frozen method were highly significant lower than that of liquid nitrogen fumigation method (P<0.01). Aspartate aminotransferase and lactate dehydrogenase activity of semen diluted were highly significant lower than the activity of frozen semen (P<0.01). The results showed that the sheep semen quality by apparatus freezing frozen method was better than the liquid nitrogen fumigation freezing method. For sperm, the release of aspartate aminotransferase was in the equilibrium phase, and the release of lactate dehydrogenase was in the freezer stage.

王新奇，苏和，达来，杨燕燕，曹贵方. 冷冻方法对杜泊绵羊冷冻精液品质的影响[J]. 中国畜牧兽医.

WANG Xin-qi, SU He, DA Lai, YANG Yan-yan, CAO Gui-fang. Effects of Freezing Methods for Dorper Sheep Frozen Semen Quality[J]. .