京海黄鸡甲状腺激素应答蛋白Spot14α基因外显子1多态性与屠体和腹脂性状的相关性分析

中国畜牧兽医

京海黄鸡甲状腺激素应答蛋白Spot14α基因外显子1多态性与屠体和腹脂性状的相关性分析

薛倩¹^，2，王金玉^1，2，张跟喜^1，2，王亚男^1，2，唐莹^1，2，魏岳^1，2，施会强³

1.扬州大学动物科学与技术学院，江苏扬州 225009；2.江苏省动物遗传繁育与分子设计重点实验室，江苏扬州 225009；3.江苏京海集团，江苏南通 226103

收稿日期:2013-12-09 出版日期:2014-07-20 发布日期:2014-08-21
通讯作者: 王金玉。E-mail: jywang@yzu.edu.cn
作者简介:薛倩(1989—），女，江苏人，硕士生，研究方向：动物遗传育种与繁殖。
基金资助:
江苏省科技支撑计划（BE2013386）；国家肉鸡产业技术体系资助（nycytx-42-G1-05）；江苏高校优势学科建设工程资助；江苏省动物遗传繁育与分子设计重点实验室资助。

Relationship between Polymorphisms of Exon 1 of THRSPα Gene and the Carcass and Abdominal Fat Traits in Jinghai Yellow Chicken

XUE Qian¹^，2,WANG Jin-yu¹^，2,ZHANG Gen-xi¹^，2, WANG Ya-nan¹^，2,TANG Ying¹^，2，WEI Yue¹^，2,SHI Hui-qiang³

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China；3. Jiangsu Jinghai Poultry Group Co., Ltd., Nantong 226103, China

Received:2013-12-09 Online:2014-07-20 Published:2014-08-21

摘要/Abstract

摘要： 本研究旨在寻找甲状腺激素应答蛋白Spot14α(thyroid hormone responsive Spot14α，THRSPα)基因的多态位点，并分析其对京海黄鸡屠体和腹脂性状产生的影响。针对THRSPα基因外显子区,利用PCR-SSCP结合测序技术对379只140日龄京海黄鸡进行SNP检测，结果发现，该基因第1外显子区存在9 bp插入缺失多态位点，形成3种基因型：AA、AB和BB，2种等位基因：A、B，基因频率分别为0.4485、0.5515。相关分析结果表明，该位点对京海黄鸡140日龄活重、屠体性状（包括屠体重、头重、脚重、翅重、胸肌重、腿肌重、全净膛重、半净膛重）和腹脂性状均没有显著影响(P>0.05)。因此推断该多态位点在京海黄鸡分子辅助标记育种中不能作为屠体和腹脂性状筛选的候选分子辅助标记。

关键词:

甲状腺激素应答蛋白Spot14α 基因；外显子；多态性；京海黄鸡；屠体和腹脂性状

Abstract: The objective of this study was to find out the SNPs of THRSPα gene and analysed their effects on the carcass and abdominal fat traits in Jinghai Yellow chicken. This experiment aimed at the exon region of THRSPα gene, using PCR-SSCP combined with sequencing technology to detect the SNPs in 379 Jinghai Yellow chickens at 140 days old. We foud that there was a 9 bp del/ins mutation in the exon 1 area of the gene. For the THRSPα gene, three genotypes(AA, AB and BB) were detected in Jinghai Yellow chicken. The frequencies of A and B alleles were 0.4485 and 0.5515, respectively. The results of the correlation analysis showed that the polymorphsim site had no significant influence(P>0.05) on the 140 day weight, carcass(including carcass weight, head weight, feet weight, wing weight, chest muscle weight, leg muscle weight, eviscerated weight, half eviscerated weight) and abdominal fat traits. Therefore,we concluded that the polymorphsim site could’t be used as a potential marker of carcass and abdominal fat traits in Jinghai Yellow chicken molecular marker-assisted breeding.

Key words:

THRSPα gene；exon；polymorphism；Jinghai Yellow chicken；carcass and abdominal fat traits

薛倩，王金玉，张跟喜，王亚男，唐莹，魏岳，施会强. 京海黄鸡甲状腺激素应答蛋白Spot14α基因外显子1多态性与屠体和腹脂性状的相关性分析[J]. 中国畜牧兽医.

XUE Qian,WANG Jin-yu,ZHANG Gen-xi, WANG Ya-nan,TANG Ying，WEI Yue,SHI Hui-qiang. Relationship between Polymorphisms of Exon 1 of THRSPα Gene and the Carcass and Abdominal Fat Traits in Jinghai Yellow Chicken[J]. China Animal Husbandry & Veterinary Medicine.

参考文献

1 王丛梅，郭豫杰，王月影，等. 猪甲状腺激素应答Spot14基因的克隆及组织表达研究［J］.中国畜牧兽医，2012，39(3):55～59.
2 闫文龙,何超,崔建勋，等. 鸡THRSPα基因多态性与生长、屠体及肉质性状的相关性［A］. 第9次全国畜禽遗传标记研讨会论文集［C］.2004.
3 闫文龙. THRSP基因对鸡脂肪沉积的影响［D］. 广州:华南农业大学, 2006.
4 闫文龙,何超,崔建勋,等. 鸡THRSPα基因多态性与脂肪性状的相关性研究［J］. 西南大学学报(自然科学版), 2007, 29(2): 57～59.
5 何超. THRSPα基因多态性及其与鸭脂肪性状的相关性研究［J］. 广东农业科学, 2010(8): 209～211.
6 张小白，昝林森，王洪宝，等. THRSP基因C184T突变与秦川牛肉质性状相关性的研究［J］. 中国农业科学，2009,42（11）：4058～4063.
7 曹志平,李辉. Spot14 基因研究进展［J］.东北农业大学学报, 2008, 39(2): 285～288.
8 詹凯,徐桂云,杨宁,等. 鸭THRSPα基因内含子多态与生长和屠体性状相关性研究［J］.中国畜牧杂志, 2007, 43(3): 1～3.
9 詹凯,杨宁,徐桂云,等. 鸡、鸭甲状腺激素应答基因(THRSP) 的研究进展［J］.遗传, 2009, 31(2): 131～136.
10 Cao Z P, Wang S Z, Wang Q G, et al. Association of Spot14 α gene polymorphisms with body weight in the chicken［J］. Poultry Science, 2007, 86: 1873～1880.
11 Carre W, Diot C, Fillon V, et al. Development of 112 unique expressed sequence tags from chicken liver using an arbitrarily primed reverse transcriptase-polymerase chain reaction and single strand conformation gel purification method［J］. Anim Genet, 2001, 32:289～297.
12 Chou W Y, Cheng Y S, Ho C L, et al. Human Spot14 protein interacts physically and functionally with the thyroid receptor［J］. Biochemical and Biophysical Research Communications, 2007, 357(1): 133～138.
13 Cogburn L A, Tang J, Cui J, et al. DNA microarray analysis of gene expression in the liver of broiler chickens divergently selected for growth rate［J］. Poult Sci, 2000, 79(Suppl 1): 72.
14 Cogburn L A, Wang X, Carre W, et al. Systems-wide chicken DNA microarrays, gene expression profiling and discovery of functional genes［J］. Poult Sci, 2003, 82: 6378～6383.
15 Cunningham B A, Maloney M, Kinlaw W B. Spot14 protein-protein interactions:Evidence for both homo- and heterodimer formation in vivo［J］.Endocrinology,1997,138(12):5184～5188.
16 Hirwa C D, Yan W, Wallace P, et al. Effects of the thyroid hormone responsive Spot14 alpha gene on chicken growth and fat traits［J］. Poultry Science, 2010, 89(9): 1981～1991.
17 Ikeobi C O, Woolliams J A, Morrice D R, et al. Quantitative trait loci affecting fatness in the chicken ［J］. Anim Genet, 2002, 33: 428～435.
18 Jump D B, Clarke S D, MacDougald O, et al. Polyunsaturated fatty acids inhibit S14 gene transcription in rat liver and cultured hepatocytes［J］. Proceedings of the National Academy of Sciences of the United States of America, 1993, 90: 8454～8458.
19 Kinlaw W, Church J, Harmon J, et al. Direct evidence for a role of the “Spot14” protein in the regulation of lipid synthesis［J］. J Biol Chem, 1995, 270:16615～16618.
20 La B, Oh D, Lee Y, et al. Association of bovine fatty acid composition with novel missense nucleotide polymorphism in the thyroid hormone-responsive(THRSP) gene［J］. Animal Genetics, 2013, 44(1): 118.
21 Lagarrigue S, Pitel F, Carre W. An initial QTL scan for abdominal fatness and breast muscle weight in broiler chickens［C］. San Diego:Plant and Animal Genome Ⅺ Conference, 2003.
22 Wang X, Carrea W, Zhou H J,et al. Duplicated Spot14 genes in the chicken: Characterization and identification of polymorphisms associated with abdominal fat traits［J］. Gene, 2004, 332:79～88.
23 Zhu Q, Anderson G W, Mucha G T, et al. The Spot14 protein is required for denovo lipid synthesis in the lactating mammary gland［J］. Endocrinology, 2005, 146: 3343～3350.