奶牛乳房炎凝固酶阴性葡萄球菌耐药性分析

中国畜牧兽医

奶牛乳房炎凝固酶阴性葡萄球菌耐药性分析

王馨宇¹，裴琳¹，于恩琪¹，林伟东¹，温馨童¹，宰栩生¹，秦爱建¹，金文杰¹，张军²，梁祥焕²，王锦荣²，杭柏林¹^，3

（1.扬州大学兽医学院，江苏省动物预防医学重点实验室，江苏省动物重要疫病与人兽共患病防控协同创新中心，江苏扬州 225009；2.扬州大学实验农牧场，江苏扬州 225009；3.河南科技学院动物科学学院，河南新乡 453003）

收稿日期:2013-12-05 出版日期:2014-06-20 发布日期:2014-07-24
通讯作者: 金文杰。E-mail: wenjiejin1@163.com 张军。E-mail:zhangjun@yzu.edu.cn
作者简介:王馨宇（1991—），女，江苏人，学士，研究方向：动物医学。裴琳（1991—），女，江苏人，学士，研究方向：动物医学。王馨宇和裴琳对本文具有同等贡献，并列为第一作者。
基金资助:
扬州大学大学生创新创业训练计划（B12205）；扬州市社会发展科技攻关项目（YZ2012114）；江苏省“青蓝工程”；江苏高校优势学科建设工程资助项目（PAPD）。

Drug-resistance Analysis of Coagulase-negative Staphylococcus Isolated from Dairy Cow Mastitis

WANG Xin-yu¹，PEI Lin¹，YU En-qi¹，LIN Wei-dong¹，WEN Xin-tong¹，ZAI Xu-sheng¹，QIN Ai-jian¹，JIN Wen-jie¹，ZHANG Jun²，LIANG Xiang-huan²，WANG Jin-rong²，HANG Bo-lin¹^，3

（1.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Key Laboratory for Animal Preventive Medicine of Jiangsu Province,College of Veterinary Medicine, Yangzhou University, Yangzhou 225009,China;2.Farm of Experiment,Yangzhou University,Yangzhou 225009,China;3.College of Animal Science,Henan Institute of Science and Technology,Xinxiang 453003,China)

Received:2013-12-05 Online:2014-06-20 Published:2014-07-24

摘要/Abstract

摘要： 本研究对江苏部分奶牛场致隐性乳房炎病原进行流行病学调查，采样并进行细菌分离培养、革兰氏染色镜检、生化鉴定，共分离到35株凝固酶阴性葡萄球菌（CNS）。用常规药敏纸片法对CNS分离菌株的抗生素耐药情况进行分析，结果显示分离培养到的35株CNS对临床常用抗生素万古霉素、左氟沙星、克拉霉素、苯唑西林、诺氟沙星、氯霉素、环丙沙星、头孢噻肟、磷霉素和呋喃妥因等高度敏感，高敏率在60%～80%之间；而对青霉素G、复方新诺明和多黏菌素B有一定的耐药性，耐药率为70%～100%。分离菌株表现了较强的多重耐药性，耐20种药物的细菌有3株，耐15种药物的细菌有1株，耐14种药物的细菌有1株，62.9%的CNS分离菌株能耐5种以上抗生素，表明导致奶牛隐性乳房炎CNS菌株耐药情况不容小视。

关键词:

奶牛乳房炎; 凝固酶阴性葡萄球菌; 药敏试验

Abstract: In this study, pathogenic epidemiology of subclinical mastitis in part of Jiangsu province dairy farms was investigated. It included sampling, bacteria cultivation, gram staining, microscopy observation and biochemical identification. 35 strains of coagulase-negative Staphylococcus (CNS) were isolated. Results of drug sensitivity test showed that 60% to 80% of CNS isolates were highly sensitive to clinical commonly used antibiotics like vancomycin, clarithromycin, benzene azole pennicillin, norfloxacin, chloromycetin, ciprofloxacin, cefotaxime, fosfomycin and nitrofurantoin. While for penicillin G, SMZ and polymyxin B, these isolates showed high drug resistance, the resistance ratio was 70% to 100%. All these CNS isolates showed multi-drug resistance, 3 CNS isolates were resistant to 20 drugs, 1 was resistant to 15 drugs，1 was resistant to 14 drugs，62.9% of these CNS isolates were resistant to more than 5 tested drugs. All these results indicated that the resistant status of CNS caused subclinical mastitis was very serious.

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">dairy cow mastitisfont-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">；coagulasefont-size: 10.5pt; mso-bidi-font-family: 宋体; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">-font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">negative Staphylococcus (CNS)font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">；drug sensitivity test

王馨宇，裴琳，于恩琪，林伟东，温馨童，宰栩生，秦爱建，金文杰，张军，梁祥焕，王锦荣，杭柏林. 奶牛乳房炎凝固酶阴性葡萄球菌耐药性分析[J]. 中国畜牧兽医.

WANG Xin-yu，PEI Lin，YU En-qi，LIN Wei-dong，WEN Xin-tong，ZAI Xu-sheng，QIN Ai-jian，JIN Wen-jie，ZHANG Jun，LIANG Xiang-huan，WANG Jin-rong，HANG Bo-lin. Drug-resistance Analysis of Coagulase-negative Staphylococcus Isolated from Dairy Cow Mastitis[J]. .

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