会理黑山羊mtDNA D-loop区遗传多样性分析

中国畜牧兽医

会理黑山羊mtDNA D-loop区遗传多样性分析

张文丽

（西昌学院动物科学学院，四川西昌 615013）

修回日期:2014-04-16 出版日期:2014-06-20 发布日期:2014-07-24
作者简介:张文丽（1974—），女，四川人，硕士，副教授，主要从事畜牧方向研究。
基金资助:
四川省教育厅重点项目（09ZA105）；四川省教育厅青年基金项目（09ZB075）；会理县畜牧局校县合作项目（XA0807）。

Genetic Diversity of Mitochondrial DNA D-loop Sequences in Huili Black Goat

ZHANG Wen-li

（Department of Animal Science, Xichang College， Xichang 615013，China）

Revised:2014-04-16 Online:2014-06-20 Published:2014-07-24

摘要/Abstract

摘要： 试验旨在研究会理黑山羊线粒体DNA (mtDNA) D-loop区的起源进化及遗传多样性，为保护该品种山羊遗传资源提供理论依据。采用PCR扩增、测序、生物信息学分析等技术分析41只会理黑山羊mtDNA D-loop区核苷酸序列，并对其进行系统发育树构建。会理黑山羊mtDNA D-loop区的长度在1211～1213 bp之间，其中2个个体为1211 bp，29个个体为1212 bp，10个个体为1213 bp；A+T(60.1%)含量远高于G+C(39.9%)含量，有9种单倍型，单倍型多样度为0.842±0.00368，核苷酸多样度为0.01542±0.00034。遗传进化分析表明，会理黑山羊与建昌黑山羊、成都麻羊、金堂黑山羊、贵州白山羊、贵州黑山羊亲缘关系最近，与旋角野山羊亲缘关系最远。会理黑山羊具有较丰富的遗传多样性，在进化过程中受到其他品种山羊影响。

关键词:

会理黑山羊；线粒体DNA Dmso-bidi-font-family: 宋体" lang="EN-US">-loop；遗传多样性

Abstract: To explore the genetic diversity and origin for genetic resource protection of Huili Black goat, the mitochondrial DNA (mtDNA) D-loop was investigated. mtDNA D-loop sequences of 41 goats were analyzed by PCR, sequencing techniques, and biological information and the phylogenetic trees were constructed. The mtDNA sequences of the Huili Black goat ranged from 1211 to 1213 bp, and 2 sequences were 1211 bp, 29 sequences 1212 bp, and 10 sequences 1213 bp. The content of A+T (60.1%) was higher than one of G+C (39.9%). There were 9 haplotypes, and the haplotype diversity was 0.842+0.00368. The nucleotide diversity was 0.01542+0.00034. The phylogenetic analysis showed that Huili Black goat was distributed in a branch, and were closed to Jianchang Black goat, Chengdu Ma goat, Jintang Black goat, Guizhou White goat, Guizhou Black goat, but they were less related to Capra falconeri. Huili Black goats had rather abundant genetic diversity, and were greatly affected by other goat breeds in history.

Key words:

Huili Black goat；mtDNA Dmso-bidi-font-family: 宋体" lang="EN-US">-loop；genetic diversity

张文丽. 会理黑山羊mtDNA D-loop区遗传多样性分析[J]. 中国畜牧兽医.

ZHANG Wen-li. Genetic Diversity of Mitochondrial DNA D-loop Sequences in Huili Black Goat[J]. .

参考文献

1 王康环, 金红, 蒋利, 等. 藏系绵羊、新疆细毛羊及阿勒泰羊mtDNA D-loop基因序列分析及系统进化研究［J］. 中国畜牧兽医, 2014, 41(1): 160～166.
2 刘若余, 杨公社, 雷初朝. 中国山羊mtDNA D-loop遗传多样性及其起源研究［J］. 遗传学报, 2006, 33(5): 420～428.
3 孙俊丽, 张冰, 马青艳, 等. 陆川猪mtDNA D-loop序列遗传多样性分析［J］. 中国畜牧兽医, 2010, 37(6): 122～125.
4 李齐发, 李隐侠, 赵兴波, 等. 牦牛线粒体DNA D-loop区序列测定及其在牛亚科中分类地位的研究［J］. 畜牧兽医学报, 2008, 39(1): 1～6.
5 李婉涛, 刘延鑫, 李新正, 等. 河南省3个山羊品种mtDNA D-loop区的序列变异与系统发育关系研究［J］. 河南农业科学, 2010, 3: 100～102.
6 於建国, 陈静波, 多来提尼牙孜·依明, 等. 和田黑鸡mtDNA控制区D-loop序列分析［J］. 中国畜牧兽医, 2011, 38(7): 105～107.
7 武建亮, 肖宇航, 刘文忠. 山羊线粒体DNA D-loop区遗传多样性研究［J］. 中国畜牧兽医, 2009, 36(5): 120～123.
8 郝荣超, 昝林森, 刘丑生, 等. 中国部分家养山羊mtDNA D环区遗传多样性与进化［J］. 遗传, 2008, 30(9): 1187～1194.
9 雷初朝, 陈宏, 杨公社, 等. 中国驴种线粒体DNA D-loop多态性研究［J］. 遗传学报, 2005, 32(5): 481～486.
10 Chen S, Fan B, Liu B, et al. Genetic variations of 13 indigenous Chinese goat breeds based on cytochrome b gene sequences［J］. Biochemical Genetics, 2006, 44(3～4): 87～97.
11 Nozawa K, Kikkawa Y, Tsunoda K, et al. Gene-constitution of the Yunnan native goat in China［J］. Report of the Society for Researches on Native Livestock, 1995, 15: 131～142.
12 Schaller G B. Mountain monarchs. Wild sheep and goats of the Himalaya［M］. Chicago：University of Chicago Press,1977.