牛源金黄色葡萄球菌FnbpA-A基因克隆及抗原表位预测

中国畜牧兽医

牛源金黄色葡萄球菌FnbpA-A基因克隆及抗原表位预测

何焱，郝永清

（内蒙古农业大学兽医学院，微生物与免疫学实验室，内蒙古呼和浩特 010018）

收稿日期:2013-11-22 出版日期:2014-05-20 发布日期:2014-06-25
通讯作者: 郝永清（1962—），男，内蒙古人，博士生导师。E-mail：haoyq1960@163.com
作者简介:何焱（1987—），女，山东人，硕士生，研究方向：兽医微生物与免疫学。
基金资助:
国家科技支撑计划项目（2011BAD18B01）。

Cloning and Antigen Epitopes Prediction of Fibronection-binding Protein A Gene A Domain of Bovine Staphylococcus aureus

HE Yan，HAO Yong-qing

(Laboratory of Microbiology and Immunology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China)

Received:2013-11-22 Online:2014-05-20 Published:2014-06-25

摘要/Abstract

摘要： 据GenBank上公布的金黄色葡萄球菌纤连蛋白结合蛋白A（fibronection-binding protein A，FnbpA）基因序列，设计1对引物特异性扩增FnbpA基因A区（FnbpA-A），然后对其进行克隆、测序、蛋白二级结构分析及抗原表位预测。结果表明，FnbpA-A基因全长1645 bp，编码548个氨基酸，与金黄色葡萄球菌CIG1835株同源性达100%；该蛋白含α螺旋13.69%、β折叠30.84%、β转角11.86%和无规则卷曲43.61%；B细胞表位可能位于N端31—46、54—69、142—157、303—318、324—339、450—465、491—506和533—548区段；T细胞表位可能位于N端353—361和245—253区段。综上所述，FnbpA-A可作为后续金黄色葡萄球菌基因工程亚单位疫苗候选基因筛选区域。

关键词: font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">金黄色葡萄球菌; FnbpAfont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">-font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Afont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">基因; 克隆; 抗原表位预测

Abstract: A pair of specific primers was designed on A domain of FnbpA gene sequence of S.aureus published in GeneBank. Then FnbpA-A gene was cloned and sequenced, its protein second structure was analyzed and antigenic epitope was predicted. The sequencing results showed that FnbpA-A gene was 1645 bp in size, encoding 548 amino acids, the homology of coding amino acids between this strain and CIG1835 strain was 100%. Protein secondary structure analysis showed that FnbpA-A protein had alpha helixes 13.69%, β sheet 30.84%, β turn 11.86% and random coil 43.61%. B-cell epitopes were most likely localized in its N-teminal 31 to 46, 54 to 69, 142 to 157, 303 to 318, 324 to 339, 450 to 465, 491 to 506, 533 to 548 sites and T-cell epitopes had the high probability in the regions of 353 to 361 and 245 to 253. To sum up, FnbpA-A could be used as a candidate gene of S.aureus genetic engineering subunit vaccine.

Key words: font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Sfont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">.aureus; FnbpAfont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">-font-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">Afont-size: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US"> gene; cloning; antigen epitopes prediction

何焱，郝永清. 牛源金黄色葡萄球菌FnbpA-A基因克隆及抗原表位预测[J]. 中国畜牧兽医.

HE Yan，HAO Yong-qing. Cloning and Antigen Epitopes Prediction of Fibronection-binding Protein A Gene A Domain of Bovine Staphylococcus aureus[J]. .

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