绵羊卵母细胞采集、体外成熟和胚胎体外培养对体外受精技术效率的影响

中国畜牧兽医

绵羊卵母细胞采集、体外成熟和胚胎体外培养对体外受精技术效率的影响

闵江涛¹^，2，杜卫华²，赵学明²，郝海生²，杭苏琴¹，朱化彬²

(1.南京农业大学动物科技学院，江苏南京 210095；2. 中国农业科学院北京畜牧兽医研究所，北京 100193)

收稿日期:2013-12-03 出版日期:2014-05-20 发布日期:2014-06-25
通讯作者: 杭苏琴，女，博士，副教授。E-mail: suqinhang69@njau.edu.cn 朱化彬，男，博士，研究员。E-mail: zhuhuabin@caas.cn
作者简介:闵江涛（1987—），男，辽宁人，硕士，研究方向：胚胎工程。
基金资助:
国家科技支撑计划(2011BAD19B02)。

Oocyte Recovery, in vitro Maturation and Embryo Culture Systems Influence the Efficiency of Ovine in vitro Fertilization

MIN Jiang-tao¹^，2, DU Wei-hua², ZHAO Xue-ming², HAO Hai-sheng²,HANG Su-qin¹, ZHU Hua-bin²

（1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China；2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China）

Received:2013-12-03 Online:2014-05-20 Published:2014-06-25

摘要/Abstract

摘要： 利用屠宰场采集的绵羊卵巢作为试验材料，研究了不同卵母细胞采集方法(卵泡冲洗法、剖切法、注射器抽吸法和真空泵抽吸法)、成熟液中添加不同来源激素(BIONICHE或宁波激素厂生产 FSH/LH)和血清(发情绵羊血清或胎牛血清)，以及mSOF和mCR胚胎培养体系对绵羊体外受精各环节效率的影响。结果表明，卵泡冲洗法获得A、B两级卵母细胞比例为77.1%，显著高于其他3种方法(P＜0.05)，添加BIONICHE FSH/LH+ESS成熟液中，卵母细胞成熟率显著高于其他添加方式(P＜0.05)，mSOF和mCR胚胎培养体系在卵裂率上无显著差异(P＞0.05)，但mSOF组中囊胚率和孵化率均显著高于mCR组(P＜0.05)。综上所述，本研究中卵泡冲洗法更适合绵羊卵母细胞采集，成熟液中添加BIONICHE FSH/LH和ESS可显著促进绵羊卵母细胞成熟；与mCR培养体系相比，mSOF培养体系更适合绵羊体外受精胚胎的发育。

关键词:

绵羊体外受精; 卵母细胞采集; 卵母细胞体外成熟

Abstract: Taking advantage of the ovaries from slaughterhouse, the effects of different kinds of oocyte recovery methods (follicle flushing, slicing, syringe aspiration and pump aspiration), different kinds of gonadotropins (FSH/LH from BIONICHE and Ningbo hormone factory) and serums (estrous sheep serum or fetal bovine serum) during in vitro maturation and different embryo culture systems (mCR and mSOF) on the efficiency of ovine in vitro fertilization were investigated. The results showed that the percentage of grade A and B oocytes recovered using the flushing method was 77.1%, which was significantly higher than using the other methods (P<0.05). In vitro maturation rate of oocytes matured in the medium supplemented with estrous sheep serum (ESS) and FSH/LH from BIONICHE was significantly higher than the other treatments (P<0.05). In mSOF and mCR cultural system, there was no significant difference in the cleavage rate between two groups （P>0.05）. However, mSOF provided significantly higher rates of blastocyst and hatched blastocyst than mCR (P<0.05). These results demonstrated that the flushing method introduced in present study was more appropriate for ovine oocyte recovery than other recovery methods；The maturation rate of ovine oocytes could be increased significantly with the supplementation of ESS and BIONICHE FSH/LH into in vitro maturation media；Compared with mCR, mSOF was superior for culturing ovine IVF embryos.

Key words:

ovine IVF; oocyte recovery; oocyte IVM

闵江涛，杜卫华，赵学明，郝海生，杭苏琴，朱化彬. 绵羊卵母细胞采集、体外成熟和胚胎体外培养对体外受精技术效率的影响[J]. 中国畜牧兽医.

MIN Jiang-tao, DU Wei-hua, ZHAO Xue-ming, HAO Hai-sheng,HANG Su-qin, ZHU Hua-bin. Oocyte Recovery, in vitro Maturation and Embryo Culture Systems Influence the Efficiency of Ovine in vitro Fertilization[J]. China Animal Husbandry & Veterinary Medicine.

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