土槿皮乙酸通过P53和caspase蛋白抑制人乳腺癌细胞MCF-7生长机制研究

中国畜牧兽医

土槿皮乙酸通过P53和caspase蛋白抑制人乳腺癌细胞MCF-7生长机制研究

田杰¹，任配友²，李锐²，刘春禹³

（1.长春医学高等专科学校，吉林长春 130031；2.吉林大学白求恩第一医院甲状腺外科，吉林长春 130000；3.长春中医药大学附属医院针灸科，吉林长春 130000）

收稿日期:2013-11-04 出版日期:2014-05-20 发布日期:2014-06-25
通讯作者: 刘春禹（1978—），男，吉林人，硕士，研究方向：针灸学和肿瘤分子药理学。E-mail: 544125048@qq.com
作者简介:田杰（1969—），女，吉林人，硕士，副教授，研究方向：天然药物有效成分及作用机理。
基金资助:
吉林省中医药管理局（2012-030）。

Study on Pseudolaric Acid B Inhibited Cell Growth through P53, Caspase 8 and Caspase 9 in MCF-7 Cells

TIAN Jie¹，REN Pei-you²，LI Rui²，LIU Chun-yu³

（1.Changchun Medical College, Changchun 130031, China; 2.Department of Thyroid Surgery,the First Hospital of Jilin University, Changchun 130000, China; 3.Acupunture Department, the Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130000, China）

Received:2013-11-04 Online:2014-05-20 Published:2014-06-25

摘要/Abstract

摘要： MCF-7细胞是caspase 3功能缺失的乳腺癌细胞，本研究结果发现土槿皮乙酸(PAB)可激活caspase 3 的上游蛋白caspase 8 和caspase 9来诱导细胞凋亡，同时PAB通过P53蛋白诱导人乳腺癌细胞MCF-7凋亡。本试验结果为进一步阐明PAB抗乳腺癌奠定了基础。

关键词:

土槿皮乙酸; P53蛋白; caspase 8; caspase 9; MCFmso-bidi-font-family: 宋体" lang="EN-US">-7细胞; 凋亡

Abstract: The assay was aimed to study the inhibitory mechanism of pseudolaric acid B (PAB) in MCF-7 cell line. MCF-7 cell was human breast cancer cell lacking of caspase 3 function. In this study, it was found that PAB activated caspase 8 and caspase 9 to induce cell apoptosis. Meanwhile PAB up-regulated P53 protein expression. Therefore PAB induced MCF-7 cell apoptosis through increasing P53 protein expression and promoting the activation of caspase 8 and caspase 9.

Key words:

pseudolaric acid B；P53；caspase 8；caspase 9；MCFmso-bidi-font-family: 宋体" lang="EN-US">-7 cell；apoptosis

田杰，任配友，李锐，刘春禹. 土槿皮乙酸通过P53和caspase蛋白抑制人乳腺癌细胞MCF-7生长机制研究[J]. 中国畜牧兽医.

TIAN Jie，REN Pei-you，LI Rui，LIU Chun-yu. Study on Pseudolaric Acid B Inhibited Cell Growth through P53, Caspase 8 and Caspase 9 in MCF-7 Cells[J]. .

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