Q热贝氏柯克斯体间接ELISA检测方法的建立

中国畜牧兽医

Q热贝氏柯克斯体间接ELISA检测方法的建立

武文君¹^，2，贾广乐^{2，廖娟红²，梅琳²，韩雪清²，林祥梅²，张映¹}

（1.山西农业大学动物科技学院，山西太谷 030801；2.中国检验检疫科学研究院，北京 100121）

收稿日期:2013-09-23 出版日期:2014-04-20 发布日期:2014-05-27
通讯作者: 贾广乐（1973—），男，山西人，副研究员。E-mail：gljiasx@hotmail.com
作者简介:武文君（1988—），女，山西人，硕士，研究方向：动物分子生物学。
基金资助:
动物Q热检疫技术及标准研究（201110247）；接壤国家边境蜱媒病生态学及流行病学合作研究（2012dfa30540-1）。

Establishment of Indirect ELISA Method to Detect Q Fever Coxiella burnetii

WU Wen-jun¹, 2,JIA Guang-le²,LIAO Juan-hong²,MEI Lin²,HAN Xue-qing²,LIN Xiang-mei²,ZHANG Ying¹

（1.College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China；2.Chinese Academy of Inspection and Quarantine,Beijing 100121,China）

Received:2013-09-23 Online:2014-04-20 Published:2014-05-27

摘要/Abstract

摘要： 本研究以Q热贝氏柯克斯体弱毒株Ⅱ相全菌为包被抗原建立Q热贝氏柯克斯体间接ELISA检测方法，通过方阵滴定法对抗原包被浓度和二抗反应浓度的确定及各项反应条件的优化，确定其阴阳性临界值为0.44。用本方法与IDEXX Q热抗体检测试剂盒对393份牛临床血清进行检测，检出的血清阳性率分别为11.45%和6.10%，符合率为94.66%。结果表明，本试验建立的检测Q热贝氏柯克斯体的间接ELISA法具有较好的特异性及较强的敏感性，可初步应用于Q热贝氏柯克斯体抗体的临床检测。

关键词: Q热; 贝氏柯克斯体; 间接ELISA; Ⅱ相抗原

Abstract: In this study, the whole bacteria protein of the Coxiella burnetii attenuated strain phase Ⅱ was used as the antigen and then the indirect ELISA to detect Q fever Coxiella burnetii was established. Through optimizing the concentration of the coated phase Ⅱ antigen, the concentration of the second antibody and the reaction condition, we confirmed that the critical value of this method was 0.44. 393 clinical bovine sera were detected by this method and IDEXX Q fever (Coxiella burnetii) Antibody Test Kit. The results showed that the sera positive detection rates were 11.45% and 6.10%, respectively. Compared with the results of the two detection methods, the coincidence was 94.66%. The results demonstrated that sensitivity and specificity of the established indirect ELISA detection method were better and it could be used for the clinical diagnosis of the antibody of Q fever Coxiella burnetii.

Key words: Q fever; Coxiella burnetii; indirect ELISA; phase Ⅱ antigen

武文君，贾广乐，廖娟红，梅琳，韩雪清，林祥梅，张映. Q热贝氏柯克斯体间接ELISA检测方法的建立[J]. 中国畜牧兽医.

WU Wen-jun,JIA Guang-le,LIAO Juan-hong2,MEI Lin,HAN Xue-qing,LIN Xiang-mei,ZHANG Ying. Establishment of Indirect ELISA Method to Detect Q Fever Coxiella burnetii[J]. .

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