华蟾毒精对B16及RMA-S细胞主要组织相容性复合体Ⅰ类分子及其相关基因表达的影响

中国畜牧兽医

华蟾毒精对B16及RMA-S细胞主要组织相容性复合体Ⅰ类分子及其相关基因表达的影响

张瑾¹，史祺云²，程莎²，曹淑芳²，常豪²，宋宇¹

修回日期:2014-01-16 出版日期:2014-04-20 发布日期:2014-05-27
通讯作者: 宋宇，女，博士，副教授，硕士生导师。E-mail：applesy@163.com
作者简介:张瑾（1988—），女，内蒙古人，硕士生，研究方向：中药抗肿瘤与肿瘤免疫。

Effects of Cinobufagin on the Major Histocompatibility Complex ClassⅠ Molecules and Expression of their Related Genes in B16 and RMA-S Cells

ZHANG Jin¹, SHI Qi-yun², CHENG Sha², CAO Shu-fang², CHANG Hao², SONG Yu¹

(1. College of Animal Science, Jilin University, Changchun 130062, China; 2. College of Veterinary Medicine, Jilin University, Changchun 130062, China)

Revised:2014-01-16 Online:2014-04-20 Published:2014-05-27

摘要/Abstract

摘要： 试验旨在考察华蟾毒精（CBG）单体对B16和RMA-S细胞MHC-Ⅰ类分子及其相关基因表达的影响。通过MTT法检测CBG对B16和RMA-S细胞生长的影响；流式细胞术检测CBG对B16和RMA-S细胞表面MHC-Ⅰ分子表达的影响；荧光定量PCR检测CBG对B16和RMA-S细胞蛋白酶体相关基因（LMP2、LMP7）和ATP结合盒（ATP-binding cassette，ABC）转运蛋白（TAP1、TAP2）基因mRNA表达的影响。结果显示，CBG对B16和RMA-S细胞MHC-Ⅰ表达的影响均不显著(P＞0.05)，但高浓度CBG可不同程度提高LMP2、LMP7、TAP1、TAP2基因mRNA表达水平。结果表明，CBG可能具有通过上调LMP2、LMP7、TAP1、TAP2基因表达水平来增强肿瘤自身免疫原性的潜能。

关键词: 华蟾毒精; 主要组织相容性复合体Ⅰ类分子; 肿瘤免疫原性

Abstract: The study was aimed to investigate the effects of cinobufagin on the major histocompatibility complex classⅠ（MHC-Ⅰ） molecules and expression of their related genes in B16 and RMA-S cells. The effect of CBG on cell proliferation was determined by MTT assay. The expression of MHC-Ⅰ molecules was assayed by flow cytometry (FCM). The mRNA expression of LMP2, LMP7, TAP1 and TAP2 genes were deteceted by Real-time PCR. The results showed that the expression of MHC-Ⅰ was not affected significantly by CBG both in B16 and RMA-S cells（P>0.05）, while the mRNA expression of LMP2, LMP7, TAP1, TAP2 genes were increased remarkably by CBG of high concentrations. CBG may have the potential to enhance the immunogenicity of tumor by means of increasing the expression of LMP2, LMP7, TAP1 and TAP2 genes.

Key words: cinobufagin; major histocompatibility complex classⅠ molecules; immunogenicity of tumor

张瑾，史祺云，程莎，曹淑芳，常豪，宋宇. 华蟾毒精对B16及RMA-S细胞主要组织相容性复合体Ⅰ类分子及其相关基因表达的影响[J]. 中国畜牧兽医.

ZHANG Jin, SHI Qi-yun, CHENG Sha, CAO Shu-fang, CHANG Hao, SONG Yu. Effects of Cinobufagin on the Major Histocompatibility Complex ClassⅠ Molecules and Expression of their Related Genes in B16 and RMA-S Cells[J]. .