miRNA-205在猪卵母细胞成熟过程中表达规律及其调控作用的研究

中国畜牧兽医

miRNA-205在猪卵母细胞成熟过程中表达规律及其调控作用的研究

陈超，李万宏，孙丽娜，陈树雄，陈璐，李纯锦，周虚

（吉林大学动物科学学院，吉林长春 130062）

收稿日期:2013-12-16 出版日期:2014-04-20 发布日期:2014-05-27
通讯作者: 周虚（1965—）, 男，广西人，博士生导师，研究方向：动物生殖调控。E-mail:xzhou65@vip.sina.com
作者简介:陈超（1988—），男，山东人，硕士，研究方向：动物生殖调控。
基金资助:
国家青年科学基金项目（31301224）。

Stydy on Expression Regulation and Regulatory Functions of miRNA-205 in Pig Oocyte in vitro Maturation

CHEN Chao，LI Wan-hong, SUN Li-na，CHEN Shu-xiong，CHEN Lu，LI Chun-jin，ZHOU Xu

（College of Animal Science, Jilin University, Changchun 130062,China）

Received:2013-12-16 Online:2014-04-20 Published:2014-05-27

摘要/Abstract

Abstract: The research aimed to assess the expression profile and regulatory functions of miRNA-205 in pig oocyte in vitro maturation. The potential target of miRNA-205 was predicted by biology software including TargetScan and microRNA.org. The expression profile of miRNA-205 and its target gene were detected by Real-time PCR in pig cumulus-oocyte complexes(COCs) in vitro maturation 0, 24 and 48 h. The expression of miRNA-205 in pig COCs maturation gradually increased, 0 h lowest, 48 h maximum. The expression of miRNA-205 was significantly reduced in mature solution added EFG, FSH and LH compared with which in a basic mature solution, the expression of PTX3 and cumulus proliferation-related genes including TNFAIP6 and HAS2 were significantly increased. The expression of PTX3 was negative correlation with that of miRNA-205 in porcine oocyte maturation. From these results could be concluded that the miRNA-205 conserved sequence specifically bound to 3′-UTR region of PTX3 gene might be participated in the regulation of oocyte maturation.

Key words: miRNA-205; cumulus-oocyte complexes; Real-time PCR; porcine oocyte maturation

陈超，李万宏，孙丽娜，陈树雄，陈璐，李纯锦，周虚. miRNA-205在猪卵母细胞成熟过程中表达规律及其调控作用的研究[J]. 中国畜牧兽医.

CHEN Chao，LI Wan-hong, SUN Li-na，CHEN Shu-xiong，CHEN Lu，LI Chun-jin，ZHOU Xu. Stydy on Expression Regulation and Regulatory Functions of miRNA-205 in Pig Oocyte in vitro Maturation[J]. .

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