猪增生性肠炎套式PCR检测方法的建立及初步应用

中国畜牧兽医

猪增生性肠炎套式PCR检测方法的建立及初步应用

张文波¹，戴益民¹，蒋新华²，胡杨¹，徐昌满¹，陈松昌²，邓舜洲¹

（1.江西农业大学动物科学技术学院，江西南昌 330045；2.南昌金牧动物保健服务有限公司，江西南昌 330013）

收稿日期:2013-10-18 出版日期:2014-04-20 发布日期:2014-05-27
通讯作者: 邓舜洲，副教授。E-mail: shzhdeng@163.com
作者简介:张文波（1972—），男，河南人，博士，研究方向：动物传染病与免疫学。
基金资助:
国家自然科学基金（31360617）；江西省科技支撑计划项目（20121BBF60031、20132BBF60044）。

Establishment and Preliminary Application of Nested PCR Method for Detection of Porcine Proliferative Enteropathy

ZHANG Wen-bo¹, DAI Yi-min¹, JIANG Xin-hua², HU Yang¹, XU Chang-man¹, CHEN Song-chang², DENG Shun-zhou¹

(1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; 2. Nanchang Jinmu Animal Health Co., Ltd., Nanchang 330013, China)

Received:2013-10-18 Online:2014-04-20 Published:2014-05-27

摘要/Abstract

摘要： 本试验旨在建立检测猪增生性肠炎(porcine proliferative enteropathy，PPE)的套式PCR方法，为临床快速鉴别诊断和防控该病提供病原学依据。根据GenBank上已发表的的劳森氏胞内菌(Lawsonia intracellularis，LI)的基因组序列（登录号：AM180252）设计2对特异性引物，以疑似PPE病猪粪便中纯化的DNA为模板，建立了套式PCR方法，并对PCR产物进行序列测定，所扩增的序列与GenBank上登录的劳森氏胞内菌相应序列的同源性在99%以上。应用本试验建立的套式PCR方法对疑似PPE病猪的粪便进行检测，结果表明，本方法具有快速、方便和敏感度高等优点，可用于PPE的临床检测。

关键词: 猪增生性肠炎; 劳森氏胞内菌; 套式PCR

Abstract: Porcine proliferative enteropathy (PPE), caused by Lawsonia intraeellularis (LI), was a kind of swine contagious infectious disease. In order to establish a nested PCR method to detect LI, two pairs of primers were designed according to the genome of LI, and the nested PCR was established for detection of LI in ileum or feces of pigs. The sequencing result analysis showed that the homologies of the amplified nucleotide sequences to the LI references sequence were above 99%. The nested PCR method established in this study was rapid, easy and sensitive, could be used in clinical diagnosis of PPE.

Key words: porcine proliferative enteropathy; Lawsonia iintracellularis; nested PCR

张文波，戴益民，蒋新华，胡杨，徐昌满，陈松昌，邓舜洲. 猪增生性肠炎套式PCR检测方法的建立及初步应用[J]. 中国畜牧兽医.

ZHANG Wen-bo, DAI Yi-min, JIANG Xin-hua, HU Yang, XU Chang-man, CHEN Song-chang, DENG Shun-zhou. Establishment and Preliminary Application of Nested PCR Method for Detection of Porcine Proliferative Enteropathy[J]. .

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