必需氨基酸上调乳腺腺泡中L型氨基酸转运载体1的表达

中国畜牧兽医

必需氨基酸上调乳腺腺泡中L型氨基酸转运载体1的表达

林叶，侯晓明，田雷，齐帮若，李庆章

（东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨 150030）

收稿日期:2013-08-28 出版日期:2014-03-20 发布日期:2014-05-15
通讯作者: 李庆章。E-mail：qzli@neau.edu.cn
作者简介:林叶（1980—），女，黑龙江人，博士，副教授，研究方向：泌乳生物学与乳腺功能调控。
基金资助:
2010年高等学校博士学科点专项科研基金联合资助课题（新教师类联合）（20102325120018）；2012年黑龙江省政府博士后科研启动金（LBH-Q12157）；东北农业大学科技人才启动基金项目（校博士启动基金）。

Essential Amino Acid Upregulates L-type Amino Acid Transporter 1 Expression in Mammary Acinus

LIN Ye, HOU Xiao-ming, TIAN Lei, QI Bang-ruo, LI Qing-zhang

(Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin 150030, China)

Received:2013-08-28 Online:2014-03-20 Published:2014-05-15

摘要/Abstract

摘要： 为探讨泌乳乳腺中必需氨基酸与L型氨基酸转运载体1（L-type amino acid transporter 1，LAT1）表达之间的关系，本研究通过体外构建奶牛乳腺泌乳模型，采用免疫印迹方法检测生理浓度的必需氨基酸对乳腺腺泡中LAT1及其辅因子4F2重链（4F2 heavy chain, 4F2hc）表达变化的影响。结果表明，生理浓度的必需氨基酸显著上调体外培养的奶牛乳腺腺泡中LAT1和4F2hc的表达，提示LAT1是奶牛乳腺上皮细胞内负责转运必需氨基酸的主要转运载体，LAT1和4F2hc的表达受必需氨基酸的诱导。

关键词: 乳腺腺泡; 必需氨基酸; L型氨基酸转运载体1（LAT1）; 4F2重链（4F2hc）

Abstract: To investigate the effect of essential amino acids on LAT1 expression in lactation mammary gland, the lactation mammary acini were cultured and LAT1 and 4F2hc expression were detected by Western blotting. The results showed that essential amino acids upregulated LAT1 and 4F2hc expression significantly in lactation mammary acini of dairy cow. It revealed that LAT1 was the mainly amino acid transporter in lactation mammary gland. The expression of LAT1 and 4F2hc was induced by essential amino acids.

Key words: mammary acinus; essential amino acid; LAT1; 4F2hc

林叶，侯晓明，田雷，齐帮若，李庆章. 必需氨基酸上调乳腺腺泡中L型氨基酸转运载体1的表达[J]. 中国畜牧兽医.

LIN Ye, HOU Xiao-ming, TIAN Lei, QI Bang-ruo, LI Qing-zhang. Essential Amino Acid Upregulates L-type Amino Acid Transporter 1 Expression in Mammary Acinus[J]. .

参考文献

1 王俊锋, 黄静, 梁国义. 泌乳反刍动物乳蛋白的合成机理及调控途径研究［J］.中国乳业，2005，26（7）: 24～27.
2 冯丽丽, 林叶, 李庆章.奶牛不同发育时期乳腺组织LAT1 和4F2hc的表达［J］.中国乳品工业，2011，39（4）: 4～7.
3 Aleman G, Lopez A, Ordaz G, et al. Changes in messenger RNA abundance of amino acid transporters in rat mammary gland during pregnancy, lactation, and weaning［J］. Metabolism, 2009, 58(5): 594～601.
4 Connor E E, Meyer M J, Li R W, et al. Regulation of gene expression in the bovine mammary gland by ovarian steroids［J］. J Dairy Sci, 2007, 90（Suppl 1）:E55～E65.
5 Drummond M J, Glynn E L, Fry C S, et al. An increase in essential amino acid availability upregulates amino acid transporter expression in human skeletal muscle［J］. Am J Physiol Endocrinol Metab, 2010, 298(5): E1011～E1018.
6 Fukasawa Y, Segawa H, Kim J Y, et al. Identification and characterization of a Na⁺-independent neutral amino acid transporter that associates with the 4F2 heavy chain and exhibits substrate selectivity for small neutral D- and L-amino acids［J］. J Biol Chem, 2000, 275(13): 9690～9698.
7 Kaira K, Oriuchi N, Imai H, et al. L-type amino acid transporter 1 and CD98 expression in primary and metastatic sites of human neoplasms［J］. Cancer Sci, 2008, 99(12): 2380～2386.
8 Manjarin R, Steibel J P, Zamora V, et al. Transcript abundance of amino acid transporters, β-casein, and α-lactalbumin in mammary tissue of periparturient, lactating, and postweaned sows［J］. J Dairy Sci, 2011, 94(7): 3467～3476.
9 Nguyen-Ngoc K V, Cheung K J, Brenot A, et al. ECM microenvironment regulates collective migration and local dissemination in normal and malignant mammary epithelium［J］. Proc Natl Acad Sci USA, 2012, 109(39): E2595～E2604.
10 O’brien L E, Zegers M M, Mostov K E. Building epithelial architecture: Insights from three-dimensional culture models［J］. Nat Rev Mol, 2002, 3(7): 531～537.
11 Ohno C, Nakanishi Y, Honma T, et al. Significance of system L amino acid transporter 1 (LAT-1) and 4F2 heavy chain (4F2hc) expression in human developing intestines［J］. Acta Histochem Cytochem, 2009, 42(3): 73～81.
12 Shennan D B, Peaker M. Transport of milk constituents by the mammary gland［J］. Physiol Rev, 2000, 80(3): 925～951.

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