›› 2014, Vol. 41 ›› Issue (12): 282-286.

• 疾病防治 • 上一篇    

长毛兔巴氏杆菌病诊断与防控

刘吉山, 姚春阳, 莫玲, 郭时金, 李峰, 付石军, 汪洋   

  1. 山东省滨州畜牧兽医研究院, 山东滨州 256600
  • 收稿日期:2014-07-02 出版日期:2014-12-20 发布日期:2014-12-25
  • 作者简介:刘吉山(1975-),男,山东人,博士生,副研究员,研究方向:预防兽医学及临床兽医诊断与治疗.
  • 基金资助:
    滨州市兽医生物技术与疫苗药品研发平台建设项目(2011GG103).

Diagnosis and Prevention of Pasteurellosis of Long-hair Rabbit

LIU Ji-shan, YAO Chun-yang, MO Ling, GUO Shi-jin, LI Feng, FU Shi-jun, WANG Yang   

  1. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China
  • Received:2014-07-02 Online:2014-12-20 Published:2014-12-25

摘要: 2013年5月从山东某大型长毛兔场患有以鼻炎和化脓性肺炎为主要临床特征的病免中分离到1株细菌,经细菌培养、染色镜检、生化试验、PCR鉴定、动物试验证实分离菌株为荚膜血清A型巴氏杆菌;药敏试验结果表明,分离菌株对替米考星、恩诺沙星、氟苯尼考、头孢噻呋钠、阿莫西林等药物敏感;通过设计3个试验组:巴氏杆菌本场疫苗免疫试验组、恩诺沙星防治试验组、巴氏杆菌本场疫苗免疫与恩诺沙星联用试验组,每组500只长毛兔,连续观察30 d,结果发现巴氏杆菌本场疫苗免疫与恩诺沙星联用试验组对该病的防控取得了较为理想的防治效果.

关键词: 长毛兔; 荚膜血清A型巴氏杆菌; PCR鉴定; 防控

Abstract: One bacterium was isolated from a large-scale Shandong Long-hair rabbit farm with the main clinical features of rhinitis and purulent pneumonia disease in June 2012. After bacterial culture, it was verified as capsular serotype A Pasteurella multocida through staining, biochemical test, PCR identification and animal test. The drug sensitivity test results showed that the bacterium was sensitive to tilmicosin, enrofloxacin, florfenicol, ceftiofur sodium and amoxicillin. 3 experimental groups (Pasteurella vaccine trial group, enrofloxacin group, Pasteurella vaccine and enrofloxacin combined group), each group with 500 Long-hair rabbits, were designed, the results showed that Pasteurella vaccine and enrofloxacin combined group had ideal effect on the prevention and control of the disease after 30 days.

Key words: Long-hair rabbit; capsular serotype A Pasteurella multocida; PCR identification; prevention and control

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