转抗仔猪腹泻基因α1-盐藻糖转移酶克隆猪的胰岛素样生长因子2表达分析

›› 2014, Vol. 41 ›› Issue (12): 1-6.

• 生物技术 • 下一篇

转抗仔猪腹泻基因α1-盐藻糖转移酶克隆猪的胰岛素样生长因子2表达分析

窦金焕¹, 李钟淑², 王雅春¹, 董易春¹, 周传丽¹, 刘铮铸¹, 张勤¹, 俞英¹

1. 中国农业大学动物科技学院, 北京 100193;
2. 延边大学农学院, 吉林延吉 133000;
3. 河北师范科技学院, 河北秦皇岛 066004

收稿日期:2014-04-04 出版日期:2014-12-20 发布日期:2014-12-25
通讯作者: 李钟淑, 俞英 E-mail:lizs1962@yahoo.com;yuying@cau.edu.cn
作者简介:窦金焕(1990－),女,内蒙古人,硕士生,研究方向:动物遗传育种.
基金资助:
国家农业部转基因重点项目资金(2009ZX08009-146B);长江学者与创新团队发展计划(IRT1191).

Expression Analysis of Imprinting Gene IGF2 in Anti-diarrhea FUT1 Transgenic Cloned Pigs

DOU Jin-huan¹, LI Zhong-shu², WANG Ya-chun¹, DONG Yi-chun¹, ZHOU Chuan-li¹, LIU Zheng-zhu¹, ZHANG Qin¹, YU Ying¹

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. Agricultural College of Yanbian University, Yanji 133000, China;
3. Hebei Normal University of Science & Technology, Qinhuangdao 066004, China

Received:2014-04-04 Online:2014-12-20 Published:2014-12-25

摘要/Abstract

摘要： 为探求转基因克隆猪存在早期死亡率高及生长发育异常等原因,本研究首次以转抗仔猪腹泻基因α1-盐藻糖转移酶(fucosyl transferase 1,FUT1)阳性及阴性克隆猪的脾脏、肝脏及腿肌组织为试验材料,利用qRT-PCR技术定量检测影响胎儿及仔猪早期生长发育的印记基因胰岛素样生长因子2(insulin-like growth factor 2,IGF2)的表达情况,并以普通妊娠分娩猪为对照,分析其差异及变化.结果显示,IGF2基因在3类仔猪中均存在组织表达差异性,其中肝脏组织中表达量最高,腿肌组织中表达量最低.分析发现,IGF2基因在转FUT1阳性克隆猪脾脏组织中的表达量显著高于转FUT1阴性克隆猪(P<0.05),在腿肌组织中则显著低于阴性克隆猪(P<0.05).此外,转FUT1阴性克隆猪的脾脏、肝脏组织IGF2表达量分别极显著低于、高于普通猪(P<0.01).提示IGF2基因的表达变化可能与克隆猪早期生活力弱有关,但与转FUT1基因克隆猪早期死亡率高的关系还需进一步研究.

关键词: 转FUT1基因克隆猪; 抗仔猪腹泻; 印记基因IGF2; 表达差异

Abstract: To explore the possible reasons for high mortality rate and early abnormal development of transgenic cloned pigs, we used anti-diarrhea transgenic (α1-fucosyl transferase gene, FUT1) positive and negative cloned pigs, as well as normal piglets as control, to analyze the expression levels of the imprinted gene insulin-like growth factor 2 (IGF2) in spleen, liver and leg muscle tissues via fluorescence quantitative RT-PCR assay. The results showed that in all three groups of pigs, there were differential expressions of IGF2 among tissues, expression level in liver tissue being the highest and that in the leg muscles being the lowest. Significant test indicated that the expression level of IGF2 in spleen tissue of FUT1 transgenic positive cloned pigs was significantly higher than that in negative cloned pigs (P<0.05), while it was significantly lower than that of negative cloned pigs in leg muscle tissue (P<0.05). Furthermore, the expression level of IGF2 in spleen of FUT1 transgenic negative cloned pigs was very significantly lower than that of normal pigs (P<0.01);However, it was very significantly higher than that of normal pigs in liver tissue (P<0.01). These results implied that the abnormal expression level of IGF2 in cloned pigs might influence their weak viability, however, its relationship with reduced survival rate of transgenic FUT1 gene cloned pigs was still needed to be defined.

Key words: transgenic FUT1 gene cloned pig; anti-diarrhea; imprinting gene IGF2; expression differences

中图分类号:

S814.8

窦金焕, 李钟淑, 王雅春, 董易春, 周传丽, 刘铮铸, 张勤, 俞英. 转抗仔猪腹泻基因α1-盐藻糖转移酶克隆猪的胰岛素样生长因子2表达分析[J]. , 2014, 41(12): 1-6.

DOU Jin-huan, LI Zhong-shu, WANG Ya-chun, DONG Yi-chun, ZHOU Chuan-li, LIU Zheng-zhu, ZHANG Qin, YU Ying. Expression Analysis of Imprinting Gene IGF2 in Anti-diarrhea FUT1 Transgenic Cloned Pigs[J]. , 2014, 41(12): 1-6.

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转抗仔猪腹泻基因α1-盐藻糖转移酶克隆猪的胰岛素样生长因子2表达分析

Expression Analysis of Imprinting Gene IGF2 in Anti-diarrhea FUT1 Transgenic Cloned Pigs

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