阿勒泰羊MyoD基因的遗传多态性及其与产肉性状的关联分析

›› 2014, Vol. 41 ›› Issue (11): 218-222.

阿勒泰羊MyoD基因的遗传多态性及其与产肉性状的关联分析

马海玉¹, 臧长江¹, 田佳², 王琼³, 于茜¹, 刘玲玲¹, 刘武军¹

1. 新疆农业大学动物科学学院, 新疆乌鲁木齐 830052;
2. 宁夏回族自治区畜牧工作站, 宁夏银川 750000;
3. 吐鲁番地区中等职业技术学校, 新疆吐鲁番 838000

修回日期:2014-10-01 出版日期:2014-11-20 发布日期:2014-12-06
通讯作者: 刘武军 E-mail:lwj_ws@163.com
作者简介:马海玉(1989-),女,新疆人,硕士生,研究方向:动物分子遗传学。
基金资助:
新疆维吾尔自治区高技术研究发展项目:新疆绵羊品种主要经济性状功能基因的鉴定(201111115)。

Association Analysis between MyoD Gene Polymorphisms and Meat Performance in Xinjiang Altay Sheep

MA Hai-yu¹, ZANG Chang-jiang¹, TIAN Jia², WANG Qiong³, YU Xi¹, LIU Ling-ling¹, LIU Wu-jun¹

1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. Livestock Workstation of Ningxia Hui Autonomous Region, Yinchuan 750000, China;
3. Turpan District Secondary Vocational and Technical School, Turpan 838000, China

Revised:2014-10-01 Online:2014-11-20 Published:2014-12-06

摘要/Abstract

摘要： 利用分子生物学手段研究MyoD基因遗传多态性与阿勒泰羊产肉量的关系,旨在为今后用分子标记辅助育种方法提高阿勒泰羊产肉性能提供科学依据。本试验以120只阿勒泰羊周岁公羊为研究对象,选取MyoD基因的5'调控区和外显子1的部分片段,采用PCR-SSCP技术检测其遗传多态性,并与周岁体重进行关联分析,研究MyoD基因的多态性与阿勒泰羊产肉性能的关系。结果表明,MyoD-P1基因座(MyoD基因5'调控区)无多态性;MyoD-P2基因座(外显子1)有多态性,存在3种基因型:AA、AB和BB,测序结果显示该处发生了碱基突变A→G,为错义突变,该突变导致其编码的氨基酸由组氨酸变为丙氨酸,经关联分析发现,该突变对阿勒泰羊产肉性能有显著影响(P<0.05)。阿勒泰羊MyoD基因外显子1上的点突变可能是影响阿勒泰羊产肉性能的重要位点,MyoD基因可望作为阿勒泰羊产肉性能的候选基因。

关键词: 阿勒泰羊; MyoD基因; 产肉量

Abstract: In this study, the relationship of MyoD gene polymorphisms and meat yield was studied by molecular biology methods. The aim was to provide scientific basis for improving meat performance of Altay sheep by molecular marker assisted breeding methods. One year old ram of Altay (n=120) were chosen in representative area to jugular vein blood, MyoD gene 5' control region and the part of the exon 1 pieces were selected to detect genetic polymorphism by PCR-SSCP, and the effects on weight traits were analyzed. It was used to research the relationship between MyoD genetic polymorphisms and meat performance. There was no polymorphism in MyoD-P1 gene locus (5' flanking region );There was polymorphism in MyoD-P2 gene locus (326-542 bp in exon 1), the primer MyoD-P2 gene locus existence three kinds of genotypes of AA, AB and BB. Sequencing results showed that there was a base mutation A→G in location 408 bp in MyoD gene exon 1. The mutation causesd its coding changing from histidine into alanine. By correlation analysis it was found that the individual weight of AA genotype were much higher than BB genotype (P<0.05). Mutational site exon 1 of MyoD gene might be the important site of Altay sheep's meat performance, MyoD gene might be the candidate genes of Altay sheep's meat performance.

Key words: Altay sheep; MyoD gene; meat yield

中图分类号:

S813.3

马海玉, 臧长江, 田佳, 王琼, 于茜, 刘玲玲, 刘武军. 阿勒泰羊MyoD基因的遗传多态性及其与产肉性状的关联分析[J]. , 2014, 41(11): 218-222.

MA Hai-yu, ZANG Chang-jiang, TIAN Jia, WANG Qiong, YU Xi, LIU Ling-ling, LIU Wu-jun. Association Analysis between MyoD Gene Polymorphisms and Meat Performance in Xinjiang Altay Sheep[J]. , 2014, 41(11): 218-222.

参考文献

1 马月辉,何晓红. 养羊业发展趋势[J]. 中国牧业通讯,2004(20):35～37.
2 王琼,朱庆. 肌肉生长相关因子MyoG基因的研究进展[J]. 黑龙江畜牧兽医,2007(4):23.
3 王琪. 凉山半细毛羊MyoD基因多态性分析及其与体重性状的相关研究[D]. 雅安:四川农业大学,2009.
4 田璐,许尚忠,岳文斌,等. MyoD基因对肉牛胴体性状影响的分析[J]. 遗传,2007,29(3):314.
5 李捷,张艳康,苏尤力,等. 新疆肉羊规模养殖与羊肉生产的发展现状分析[J]. 草食家畜,2013(1):1～8.
6 李景芬,刘娣,于浩. 7个猪种MyoD基因家族中3个基因外显子的SNPs检测分析[J]. 中国畜牧杂志,2005(5):21～24.
7 张冀汉. 中国养羊业现状及发展对策(上)[J]. 草食家畜,2003(1):1～4.
8 张海军,陈宏,房兴堂,等. 山羊MyoD基因家族多态性及与体尺性状的相关性[J]. 遗传,2007,29(9):1077～1082.
9 褚敏,阎萍,梁春年,等. 大通牦牛MyoD1基因3'UTR SNPs多态性及其与生长性状相关性的研究[J]. 中国畜牧兽医, 2012, 39(2): 111～113.
10 Edmondson D. Helix-loop-helix proteins as regulatorys of muscle-specific transcription[J]. Biol Chem,1993,268:755～758.
11 Hughes S M,Schiaffino S. Control of muscle fiber size:Acrucial factor in ageing[J]. Acta physiological Scandinavica,1999,167:307～312.
12 Pas M F,Visscher A H. Genetic regulation of meat production by embryonic muscle formation——A review [J]. Anima Breed Genet,1994,111:404～412.
13 Stratil A,Cepica S. Three polymorphisms in the porcine myogenic factor 5(MYF5) gene detected by PCR-RFLP[J]. Animal Genetic,1999,30(1):79～80.
14 te Pas M F,Harders F,Soumillion A,et al. Genetic variation at the porcine MYF-5 gene locus. Lack of association with meat production traits[J]. Mammalian Genome,1999,10(2):123～127.
15 Yablonka R Z,Rudnicki M A,Rivera A J,et al. The transition from proliferation to differentiation is delayed in satellite cells from mice lacking MyoD[J]. Dev Biol,1999,210(2):440～445.

[1]	麦麦提依明·麦麦提力, 叶梦珺, 候萌, 王俊丽, 唐安琪, 向微微, 柳荦, 王金泉, 胡燕. 阿勒泰羊生长速度与主要血液生化指标含量及尾脂Leptin基因表达关系[J]. 中国畜牧兽医, 2021, 48(7): 2369-2377.
[2]	张丽君, 陆壮, 李静, 金双勇, 王春润, 蒋磊. 辽育白牛胴体产肉量预测模型研究[J]. , 2016, 43(6): 1654-1658.
[3]	田佳，周卫东，马晓燕，郑文新，高维明，迪拉热?沙塔尔，左晓佳，师帅，张蓉银，刘武军. 新疆阿勒泰羊钙蛋白酶抑制蛋白基因多态性与嫩度的关联性分析[J]. 中国畜牧兽医, 2014, 41(6): 177-181.
[4]	杨莉, 齐亚银, 张莉, 沈文, 李岩, 张鲁安. 冷应激对新疆阿勒泰羊脂蛋白脂酶mRNA表达的影响[J]. , 2014, 41(12): 230-234.
[5]	田发益;陈大芳;路永强. 多浪羊和阿勒泰羊发情周期测定[J]. , 2008, 1(11): 156-157.
[6]	杨会国;冯克明;候广田;郝耿;艾比西;薛正芬;古丽莎拉;卡拉提;古丽格娜. 道赛特、萨福克与阿勒泰羊杂交公羔育肥效果对比试验[J]. , 2007, 34(3): 130-132.