酶定向进化方法的研究进展

中国畜牧兽医 ›› 2013, Vol. 40 ›› Issue (8): 68-71.

酶定向进化方法的研究进展

张林, 朱小翌, 江明锋

西南民族大学生命科学与技术学院, 动物遗传育种学国家民委—教育部重点实验室, 四川成都 610041

收稿日期:2013-01-22 出版日期:2013-08-20 发布日期:2013-08-16
通讯作者: 江明锋 E-mail:mingfengjiang@vip.sina.com
作者简介:张林(1988- ),男,四川人,硕士生,研究方向:遗传学。
基金资助:
四川省青年科技基金(09ZQ026-011);教育部科学技术研究重点项目(210265);2011年度四川省学术和技术带头人培养资金资助(2011-1);新津县科技局科研项目(2011-2)。

Research Progress on Directed Evolution of Enzymes

ZHANG Lin, ZHU Xiao-yi, JIANG Ming-feng

Key Laboratory of Animal Genetics & Breeding, State Ethnic Affairs Commission and Ministry of Education, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China

Received:2013-01-22 Online:2013-08-20 Published:2013-08-16

摘要/Abstract

摘要： 酶作为一种生物催化剂,已广泛应用于生活和生产中,但能直接应用于工业生产的天然酶并不多,因此,需要对酶进行人工改造。分子定向进化是在实验室模拟自然进化过程的一种技术,已广泛用于酶的改造中,并取得了较好的成绩。作者将简要介绍酶定向进化的原理、方法及应用,并展望其发展前景。

关键词: 定向进化; 化学诱变; 易错PCR; DNA改组; 随机引发体外重组

Abstract: As a biological catalyst, enzymes had been widely used in industrial production. But few of nature enzymes could be directly used for industrial production. So, nature enzymes needed be improved. Directed evolution was a molecular biology technique that allowed natural evolutionary processes to be mimicked in the laboratory, it was widely used to improve enzymes and achieved success. This article would be briefly described the methods and applications of directed evolution,meanwhile, the development of directed evolution was also prospected.

Key words: directed evolution; chemical mutagenesis; error-prone PCR; DNA shuffling; random-priming recombination

中图分类号:

Q814.9

张林, 朱小翌, 江明锋. 酶定向进化方法的研究进展[J]. 中国畜牧兽医, 2013, 40(8): 68-71.

ZHANG Lin, ZHU Xiao-yi, JIANG Ming-feng. Research Progress on Directed Evolution of Enzymes[J]. , 2013, 40(8): 68-71.

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