添加阿拉伯半乳聚糖或聚蔗糖对牛取样胚胎冷冻效果的影响

中国畜牧兽医 ›› 2013, Vol. 40 ›› Issue (8): 133-136.

添加阿拉伯半乳聚糖或聚蔗糖对牛取样胚胎冷冻效果的影响

韩伟¹, 冯春涛², 毕江华², 陈龙¹, 刘潇¹, 裴燕², 余文莉²

1. 石家庄天泉良种奶牛有限公司, 河北石家庄 050051;
2. 北京安伯胚胎生物技术中心, 北京 100107

收稿日期:2012-11-28 出版日期:2013-08-20 发布日期:2013-08-16
通讯作者: 余文莉
作者简介:韩伟(1985- ),女,河北人,硕士,研究方向:胚胎生物技术。
基金资助:
国家科技支撑计划课题"家畜胚胎保存与性控双犊诱导技术研究与示范"(2011BAD19B01);河北省重大技术创新项目(11220403Z);石家庄市重大科技课题(11150142A)。

The Effect of Adding Arabogalactan or Ficoll on Freezing Liquid in the Refrigerating of Cattle Biopsied Embryos

HAN Wei¹, FENG Chun-tao², BI Jiang-hua², CHEN Long¹, LIU Xiao¹, PEI Yan², YU Wen-li²

1. Shijiazhuang Tianquan Elite Dairy Ltd., Shijiazhuang 050051, China;
2. Beijing Anbo Embryo Biotech Center, Beijing 100107, China

Received:2012-11-28 Online:2013-08-20 Published:2013-08-16

摘要/Abstract

摘要： 胚胎取样后由于透明带受损、细胞数减少使得冷冻存活率低于常规胚胎,本试验通过在冷冻液中添加聚蔗糖和阿拉伯半乳聚糖分析其对取样胚胎冷冻效果的影响。通过将各种冷冻液的胚胎回收率、培养24和48 h发育率同对照组进行比较,结果发现,在阿拉伯半乳聚糖系列冷冻液中,0.03 EAS₁冷冻效果最好;在聚蔗糖系列冷冻液中,0.6 EFS₁冷冻效果最好,且移植妊娠结果同培养结果一致。

关键词: 取样胚胎; 阿拉伯半乳聚糖; 聚蔗糖; 冷冻液

Abstract: Owing to harmed zona pellucida and decreased cell number after sampled, the survival rate of freezed embryos was lower than those from normal ones. The effect of freezed embryos by addition of ficoll and arabinogalactan in freezing liquid was analyzed in this paper. The embryo recoveries,developmental rate after culturing 24 and 48 h of all kinds of refrigerating fluid were compared with control group,and the final result was derived, 0.03 EAS₁ was the best refrigerating fluid in arabogalactan series refrigerating fluid, and 0.6 EFS₁ was the best refrigerating fluid in ficoll series refrigerating fluid, transplantation results in line with culturing.

Key words: sampled embryos; arabogalactan; ficoll; refrigerating fluid

中图分类号:

S814.3

韩伟, 冯春涛, 毕江华, 陈龙, 刘潇, 裴燕, 余文莉. 添加阿拉伯半乳聚糖或聚蔗糖对牛取样胚胎冷冻效果的影响[J]. 中国畜牧兽医, 2013, 40(8): 133-136.

HAN Wei, FENG Chun-tao, BI Jiang-hua, CHEN Long, LIU Xiao, PEI Yan, YU Wen-li. The Effect of Adding Arabogalactan or Ficoll on Freezing Liquid in the Refrigerating of Cattle Biopsied Embryos[J]. , 2013, 40(8): 133-136.

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