不同来源柔嫩艾美耳球虫虫株微线体3基因的克隆和序列分析

›› 2013, Vol. 40 ›› Issue (6): 31-37.

不同来源柔嫩艾美耳球虫虫株微线体3基因的克隆和序列分析

王新秋¹, 高艳¹, 吴林林¹, 刘少芬¹, 刘丽丹², 翁亚彪¹, 林瑞庆¹

1. 华南农业大学兽医学院, 广东广州 510642;
2. 佛山市正典生物技术有限公司, 广东佛山 528138

收稿日期:2012-10-15 出版日期:2013-06-20 发布日期:2013-06-20
通讯作者: 林瑞庆 E-mail:rqlin@scau.edu.cn
作者简介:王新秋(1987-),男,福建人,硕士生,研究方向:兽医寄生虫学。
基金资助:
广东省科技计划项目(2012A020100001);家畜疫病病原生物学国家重点实验室开放课题(SKLVEB2012KFKT003)。

Cloning and Sequence Analysis of Microneme Proteins 3 Gene of Different Eimeria tellena Strains

WANG Xin-qiu¹, GAO Yan¹, WU Lin-lin¹, LIU Shao-fen¹, LIU Li-dan², WENG Ya-biao¹, LIN Rui-qing¹

1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2. Foshan Standard Bio-Tech Co., Ltd., Foshan 528138, China

Received:2012-10-15 Online:2013-06-20 Published:2013-06-20

摘要/Abstract

摘要： 以8株不同来源柔嫩艾美耳球虫分离株为研究对象,对其艾美耳球虫微线体3(Eimeria tenella microneme protein,EtMIC3)基因进行了RT-PCR扩增、测序及序列分析,并与GenBank上已发表的柔嫩艾美尔球虫相关序列进行比较,研究EtMIC3基因遗传变异情况。结果获得2976 bp的目的片段,各株与FJ374765.1 CDs的序列相似性在99.8%~99.9%之间,各株之间的序列相似性为99.9%~100.0%,各株编码的氨基酸序列与GenBank登记的柔嫩艾美耳球虫EtMIC3蛋白(ACJ11219)相似性在99.6%~99.9%之间,不同地理来源或耐药性虫株之间没有明显差异。本研究首次对中国EtMIC3基因进行了序列分析,结果显示不同来源株EtMIC3基因高度保守,为有效的疫苗候选因子,为进一步进行柔嫩艾美耳球虫基因工程疫苗构建的研究奠定了基础。

关键词: 柔嫩艾美球虫; RT-PCR; 微线体3; 序列分析

Abstract: The gene coding for Eimeria tenella microneme protein 3 (EtMIC3) of different strains was amplified by RT-PCR, subsequently cloned and analysed, and compared with related sequences published on GenBank. The results showed that the EtMIC3 sequence of 8 different strains was 2976 bp in length, with little difference in each other. The sequence similarities of nucleotide were 99.8% to 99.9% compared with FJ374765.1 CDs available in GenBank, and 99.9% to 100.0% within each of EtMIC3 obtained in this study with no obvious difference between strains of different geographical origin or drug resistance. The sequence similarities of induced amino acid were 99.6% to 99.9% compared with EtMIC3 (ACJ11219) available in GenBank. It was the first report on the analysis of EtMIC3 sequences of different Eimeria tenella strains from China. The results revealed EtMIC3 was high conservative, and could be used as valid vaccine candidate for the further study on constructing genetic engineering vaccine.

Key words: Eimeria tenella; RT-PCR; microneme proteins 3; sequence analysis

中图分类号:

S852.72

王新秋, 高艳, 吴林林, 刘少芬, 刘丽丹, 翁亚彪, 林瑞庆. 不同来源柔嫩艾美耳球虫虫株微线体3基因的克隆和序列分析[J]. , 2013, 40(6): 31-37.

WANG Xin-qiu, GAO Yan, WU Lin-lin, LIU Shao-fen, LIU Li-dan, WENG Ya-biao, LIN Rui-qing. Cloning and Sequence Analysis of Microneme Proteins 3 Gene of Different Eimeria tellena Strains[J]. , 2013, 40(6): 31-37.

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