薄层层析－紫外分光光度法测定预混剂中的恩拉霉素

›› 2013, Vol. 40 ›› Issue (4): 125-128.

薄层层析－紫外分光光度法测定预混剂中的恩拉霉素

周鹏飞¹, 陈敏¹, 方中²

1. 浙江工商大学食品与生物工程学院,浙江杭州 310012;
2. 浙江凯胜科技有限公司,浙江杭州 310014

收稿日期:2012-09-20 出版日期:2013-04-20 发布日期:2013-04-19
通讯作者: 陈敏,博士,教授。E-mail:365773536@qq.com E-mail:365773536@qq.com
作者简介:周鹏飞(1988-),男,江西人,硕士生,研究方向:生物化工。
基金资助:
兽用抗生素研发及剂形研究开发(技咨037/2011)。

Determination of Enramycin in Premix by TLC-UV Spectrophotometry

ZHOU Peng-fei¹, CHEN Min¹, FANG Zhong²

1. College of Food Science & Bioengineering,Zhejiang Gongshang University, Hangzhou 310012,China;
2. Zhejiang Chyszern technology Co.,Ltd.,Hangzhou 310014,China

Received:2012-09-20 Online:2013-04-20 Published:2013-04-19

摘要/Abstract

摘要： 试验旨在建立薄层层析－紫外分光光度法测定预混剂中恩拉霉素的方法。使用硅胶GF254薄层板为固定相,正丁醇∶冰醋酸∶水(2∶1∶1)为展开剂,在紫外光灯(254 nm)下定位,样品中的恩拉霉素得到较好分离。在271 nm波长处对不同含量的恩拉霉素标品进行紫外检测,结果表明,恩拉霉素点样量在20~166 μg范围内与吸光度呈现良好的线性关系(r=0.9989),平均回收率为99.85%,RSD值为3.1%(n=9)。此法所测定的结果与高效液相色谱(HPLC)法所测定的结果基本一致。该方法简单、快捷、准确、分析量大,适用于预混剂中恩拉霉素的定量分析。

关键词: 恩拉霉素; 薄层层析－紫外分光光度法; 预混剂

Abstract: A method of TLC-UV was set up for determining enramycin in premix. Target components were extracted by acetone aqueous solution. Using TLC plate, a mixture of N-Butanol∶glacial acetic acid∶water (2∶1∶1) was used as developer. Fixing the position under the light of a UV lamp(254 nm), detection wavelength was 271 nm. The absorbance and quantity of sample application showed a good linear relationship when the quantity of enramycin ranged from 20 to 166 μg (r=0.9989) with average recovery of 99.85% and RSD value of 3.1%(n=9). The content of enramycin in premix detected by this method was almost the same as that was detected by HPLC. This method which was simple, rapid and accurate,large amount of analysis,could be used to the quantitative analysis of enramycin in premix.

Key words: enramycin; TLC-UV spectrophotometry; premix 

中图分类号:

S859.79⁺6

周鹏飞, 陈敏, 方中. 薄层层析－紫外分光光度法测定预混剂中的恩拉霉素[J]. , 2013, 40(4): 125-128.

ZHOU Peng-fei, CHEN Min, FANG Zhong. Determination of Enramycin in Premix by TLC-UV Spectrophotometry[J]. , 2013, 40(4): 125-128.

参考文献

1 刘雅妮,顾欣,蔡金华. 恩拉霉素预混剂含量测定方法的改进[J]. 中国兽药杂志,2006,40(4):29~31.
2 李正.阿维拉霉素高产菌的选育与发酵工艺的研究.武汉:武汉工业学院,2010.
3 朱明华.仪器分析[M]. 第4版.北京:高等教育出版社,2008.
4 陈敏,金萍,方中. 恩拉霉素微生物检定方法的研究[J].中国畜牧兽医,2012,39(1):56~59.
5 周岷江,严玉宝,胡娟,等. 恩拉霉素的研究进展[J]. 中国兽药杂志, 2007, 41(12): 48~50.
6 姜典镇,刘松辉,朱素霞,等.薄层层析分离－紫外分光光度法测定冰清中的环丙沙星[J].化学研究,2001,12(2):46~48.
7 顾欣,蔡金华. 中华人民共和国国家标准《饲料中恩拉霉素的测定微生物学法》[M].北京:中国标准出版社, 2008a.
8 顾欣,蔡金华,刘雅妮. 饲料中恩拉霉素的微生物学含量测定方法研究[J]. 中国兽药杂志,2008b,42(9):17~20.
9 戴静,方娜娜. 薄层层析－紫外分光光度法测定十全大补胶囊中芍药苷含量[J]. 天然产物研究与开发, 2003,15(3):206~207.
10 Abdel-Moety E M, Al-Obaid A M, Jado A I,et al. Coupling of TLC and UV measurement for quantification of naproxen and its main metabolite in urine[J]. European Journal of Drug Metabolism and Pharmacokinetics, 1988, 13(4):267~271.
11 Asai M, Muroi N, Sugita H, et al. Enduracidin. A new antibiotic Ⅱ. Isolation and characterization [J]. Antibiotics, 1968,2(21):138~146.
12 Castiglione F, Marazzi A, Meli M. Structure elucidation and 3D solution conformation of the antibiotic enduracidin determined by NMR spectroscopy and molecular dynamics[J]. Magn Reson Chem,2005, 43: 603~610.
13 Hatano K, Nogami I, Higashide E. Biosynthesis of enduracidin: origin of enduracididine and other amino acids[J]. Agriculture Biochemistry, 1984, 8(6):1503~1508.
14 Higashide E, Hatano K, Shibata M, et al. Enduracidin. A new antibiotic Ⅰ, Streptomyces fungicidicus No. B5477, an enduracidin producing organism [J]. J Antibiotics, 1968, 21(2):126~137.
15 Iwasaki H. A New Antibiotic Ⅷ structures of enduracidins A and B[J]. Chemical and Pharmaceutical Bulletin, 1973, 21(6): 1184~1191.
16 Liu L,Ao L,Song J. Determination of daphnetin in girald daphne bark fumigant by TCL-UV spectrophotometry[J]. China Pharmacy,2007,18(15):1171~1173.
17 Xihou Y T, Zabriskie M. The enduracidin biosynthetic gene cluster from Streptomyces fungicidicus[J]. Microbiology, 2006, 152: 2969~2983.

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