动物性食品源大肠杆菌O血清型鉴定及其K88菌毛基因检测

›› 2013, Vol. 40 ›› Issue (3): 185-188.

动物性食品源大肠杆菌O血清型鉴定及其K88菌毛基因检测

张艳英, 高桂生, 史秋梅, 高光平, 刘申

河北科技师范学院,河北省预防兽医学重点实验室,河北秦皇岛 066600

收稿日期:2012-07-28 出版日期:2013-03-20 发布日期:2013-03-19
通讯作者: 史秋梅(1965-),女,河北人,教授,博士,研究方向:动物传染病学。E-mail:shiqiumei@yahoo.com.cn E-mail:shiqiumei@yahoo.com.cn
作者简介:张艳英(1974-),女,河北人,副教授,硕士,研究方向:动物病原微生物。
基金资助:
科技部国家农业成果转化项目(2012GB2420045);中国博士后基金(20100470565);河北省科技支撑计划(10960408D);秦皇岛市科技发展计划(201101A182)。

O Serotype Identification and K88 Fimbriae Genes Analysis of E.coli Isolated from Animal Foods

ZHANG Yan-ying, GAO Gui-sheng, SHI Qiu-mei, GAO Guang-ping, LIU Shen

Key Laboratory of Preventive Veterinary Medicine of Hebei Province, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China

Received:2012-07-28 Online:2013-03-20 Published:2013-03-19

摘要/Abstract

摘要： 本研究对河北省冀东地区农贸市场和超市采集的生猪肉、生鸡蛋和生羊肉等分离得到的20株大肠杆菌进行大肠杆菌血清型鉴定;并检测不同血清型大肠杆菌的K88菌毛基因。采用常规方法进行大肠杆菌的O血清型鉴定,用PCR方法检测K88菌毛基因。分离鉴定的20株大肠杆菌有7种血清型,包括O38、O78、O88、O11、O107、O91、O9,其中O38、O78为优势血清型菌,均占分离菌株的25%(5/20)。在分离的动物性食品源大肠杆菌中有30%(6/20)的菌株K88菌毛基因扩增呈阳性。结果表明,O78、O38为冀东地区动物性食品源大肠杆菌常见血清型,30%(6/20)的菌株K88菌毛基因扩增阳性。

关键词: 动物性食品源大肠杆菌; O血清型; K88基因

Abstract: 20 strains of E.coli from meat, chickens and mutton collected from product market and supermarket of Hebei Jidong areas were to be identified with serotype identification and K88 fimbriae gene of different serotypes was detected. O serotype of the strains of E.coli were identified with conventional methods and PCR. These isolates distributed in 7 serogroups (O38,O78,O88,O11,O107,O91,O9) and O38 and O78 were the dominant serotypes, the positive rates were both 25%(5/20). Detection rate of K88 fimbriae gene from food was 30%(6/20).The serotypes of O78 and O38 in Jidong areas from food were commom, and the detection rate of K88 fimbriae gene from food was 30%.

Key words: E.coli from animal food; O serotype; K88 gene

中图分类号:

S852.612

张艳英, 高桂生, 史秋梅, 高光平, 刘申. 动物性食品源大肠杆菌O血清型鉴定及其K88菌毛基因检测[J]. , 2013, 40(3): 185-188.

ZHANG Yan-ying, GAO Gui-sheng, SHI Qiu-mei, GAO Guang-ping, LIU Shen. O Serotype Identification and K88 Fimbriae Genes Analysis of E.coli Isolated from Animal Foods[J]. , 2013, 40(3): 185-188.

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