B亚群禽白血病病毒SDAU09C2株的TCID50与p27抗原之间的相关性研究

›› 2013, Vol. 40 ›› Issue (2): 14-17.

B亚群禽白血病病毒SDAU09C2株的TCID₅₀与p27抗原之间的相关性研究

李薛¹, 董宣¹, 赵鹏¹, 牛星², 崔治中¹

1. 山东农业大学动物科技学院,山东省畜禽疫病防制工程技术研究中心,山东泰安 271018;
2. 山东畜牧兽医职业学院,山东济南 250000

收稿日期:2012-07-31 出版日期:2013-02-20 发布日期:2013-02-06
通讯作者: 崔治中(1944-),男,江苏人,博士生导师,主要从事分子病毒学研究。Tel:0538-8241560;E-mail:zzcui@sdau.edu.cn E-mail:zzcui@sdau.edu.cn
作者简介:李薛(1988-),男,山东人,硕士生,研究方向:分子病毒学。
基金资助:
国家公益性行业(农业)科研专项种鸡场禽白血病防控与净化技术的集成(#201203055)经费。

Study of the Correlation between TCID₅₀ and p27 Antigen of Subgroup B Avian Leukosis Virus

LI Xue¹, DONG Xuan¹, ZHAO Peng¹, NIU Xing², CUI Zhi-zhong¹

1. Shandong Engineering Research Center for Animal Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
2. Shandong Vocational Animal Science and Veterinary College, Jinan 250000, China

Received:2012-07-31 Online:2013-02-20 Published:2013-02-06

摘要/Abstract

摘要： 为了研究B亚群禽白血病病毒(ALV-B)的半数细胞培养物感染量(TCID₅₀)与p27抗原的酶联免疫吸附试验(ELISA)的S/P值之间的相关性,本试验将ALV-B SDAU09C2 株接种鸡胚成纤维细胞(CEF细胞),换维持液后连续10 d取样,检测10 d的TCID₅₀值与p27抗原的S/P值之间的相关性;同时,将该毒株在DF-1细胞系上传代至20代,取其中的第1、5、10、15和20代分别进行TCID₅₀滴度的测定和p27抗原检测。结果表明,在CEF细胞上接种的ALV-B SDAU09C2株连续10 d的TCID₅₀值与p27抗原之间存在显著的正相关(r=0.94002;P<0.0001);在DF-1细胞系上传的不同代数之间也呈显著正相关(r=0.96449;P=0.0080)。由此可推测ALV-B的TCID₅₀与p27抗原呈显著正相关,可以用ELISA法测得的p27抗原的S/P值来估测病毒的TCID₅₀值。

关键词: B亚群禽白血病病毒; TCID₅₀; p27抗原; Pearson相关

Abstract: To study the correlation between 50% tissue-culture infective dose (TCID₅₀) value and p27 antigen's S/P value of subgroup B avian leukosis virus and discuss their significance, chicken embryo fibroblast (CEF) cells were inoculated with subgroup B avian leukosis virus strain SDAU09C2 and samples were taken continuously for ten days after changing maintenance media. The correlations between TCID₅₀ value and p27 antigen's S/P value of ten days were then detected. Simultaneously, DF-1 cells were inoculated with it and passed to 20 generations. Samples taken from 1st, 5th, 10th, 15th and 20th generation were received the TCID₅₀ titer determination and the p27 antigen examination separately. A significant Pearson correlation was found between them in CEF cells (r=0.85277; P<0.0001) and in DF-1 cells (r=0.93000; P=0.0220). This study provided an important parameter for predicting TCID₅₀ by detecting the p27 antigen's S/P value.

Key words: subgroup B avian leukosis virus; TCID₅₀; p27 antigen; Pearson correlationMYCBiol,2012,Epub ahead of print)

中图分类号:

S816.79

李薛, 董宣, 赵鹏, 牛星, 崔治中. B亚群禽白血病病毒SDAU09C2株的TCID₅₀与p27抗原之间的相关性研究[J]. , 2013, 40(2): 14-17.

LI Xue, DONG Xuan, ZHAO Peng, NIU Xing, CUI Zhi-zhong. Study of the Correlation between TCID₅₀ and p27 Antigen of Subgroup B Avian Leukosis Virus[J]. , 2013, 40(2): 14-17.

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B亚群禽白血病病毒SDAU09C2株的TCID₅₀与p27抗原之间的相关性研究

Study of the Correlation between TCID₅₀ and p27 Antigen of Subgroup B Avian Leukosis Virus

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