中国草原红牛肌肉生长抑制素基因3’-UTR多态性及其与屠宰性状的关联性分析

›› 2013, Vol. 40 ›› Issue (10): 184-188.

中国草原红牛肌肉生长抑制素基因3’-UTR多态性及其与屠宰性状的关联性分析

郭振刚^1,2, 张立春², 曹阳², 于永生², 罗晓彤², 金海国²

1. 吉林农业大学动物科学技术学院, 吉林长春 130000;
2. 吉林省农业科学院畜牧分院, 吉林公主岭 136100

修回日期:2013-08-12 出版日期:2013-10-20 发布日期:2013-12-19
通讯作者: 金海国 E-mail:khk1962@126.com
作者简介:郭振刚(1986-),男,贵州人,硕士生,研究方向:分子遗传与动物育种。
基金资助:
家养动物种质资源平台。

Correlations Analysis between Polymorphism of 3’-UTR in MSTN Gene and Slaughter Traits of China Red Steppe

GUO Zhen-gang^1,2, ZHANG Li-chun², CAO Yang², YU Yong-sheng², LUO Xiao-tong², JIN Hai-guo²

1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130000, China;
2. Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling 136100, China

Revised:2013-08-12 Online:2013-10-20 Published:2013-12-19

摘要/Abstract

摘要： 为研究肌肉生长抑制素(myostatin,MSTN)基因对中国地方黄牛品种产肉性能的影响,本试验以吉林省2个地方黄牛品种(草原红牛、延边黄牛)为研究对象,以4个二元杂交黄牛群体(德国黄牛×西门塔尔、利木赞×西门塔尔、夏洛莱×西门塔尔、红安格斯×西门塔尔)为参照,采用限制性片段长度多态性聚合酶链式反应(PCR-RFLP)技术对MSTN基因的多态性进行分型,并将其与草原红牛屠宰性状进行关联分析。测序结果发现,在MSTN基因外显子3上没有发现突变位点,而3'-UTR区发现了C5357A突变,导致了自然酶切位点AclⅠ基因的消失;6个群体皆表现多态性,形成A和C 2个等位基因和AA、CA、CC 3种基因型;4个二元杂交群体的AA基因型频率明显高于2个地方群体,且等位基因A为优势等位基因;CA、CC和AA基因型频率在二元杂交群体间和地方群体间的频率分布变异不大,等位基因C在地方群体间的基因频率明显高于等位基因A,为地方群体的优势等位基因。将草原红牛不同基因型与部分屠宰性能进行关联分析,结果表明AA基因型个体的净肉率和眼肌面积显著高于CA和CC基因型个体(P<0.05),但其他的屠宰性能在3种基因型上无显著差异(P>0.05)。上述结果可为今后草原红牛在产肉性能上的分子育种研究提供参考。

关键词: 中国草原红牛; 肌肉生长抑制素基因; 关联性分析

Abstract: In order to study the effect of myostatin (MSTN) gene on the meat performance of Local Yellow cattle in China,selecting 2 Local cattle breeds (Grassland Red bull,Yanbian cattle) as the research object,four binary hybrid cattle group (Germany Yellow cattle×Simmental,Limousin×Simmental,Charolais×Simmental and Red Angus×Simmental) as reference.PCR-RFLP and DNA sequencing methods were conducted to detect single nucleotide polymorphism of MSTN gene in two Chinese native cattle and four cross cattle,and analyzed the correlations between single nucleotide polymorphism of China Red Steppe MSTN gene and slaughter traits.The sequence analysis showed that there was no mutations in the MSTN gene exon 3,a C5358A mutation of 3'-UTR of MSTN gene was found,which made the AclⅠrestriction enzyme site disappeared,all cattle groups showed polymorphism which was formed two genes as A,C, and 3 genotypes as AA,CA and CC, respectively.The research results suggested that AA genotype in groups of four hybrid cattle had dominant advantage and the genotype frequency was apparently higher than two groups of Chinese Native cattle for the test of independence,allele A was expressed the preferential allelic,the frequencies of CA,CC and AA genotypes showed a little variation between two cross cattle and Native cattle,and the frequency of allele C was apparently higher than allele A,so allele C was designed the preferential allelic in the local population.While correlations between different genotypes of China Red Steppe and slaughter traits analysis showed that AA genotype individual net meat percentage and eye muscle area were significantly higher than CA and CC genotype individuals (P<0.05),but other slaughter straits had no significant differences in three genotypes(P>0.05).These results provided a useful reference for research of the meat performance of China Red Steppe in the molecular breeding field.

Key words: China Red Steppe; MSTN gene; correlation analysis

中图分类号:

S343.1⁺5

郭振刚, 张立春, 曹阳, 于永生, 罗晓彤, 金海国. 中国草原红牛肌肉生长抑制素基因3’-UTR多态性及其与屠宰性状的关联性分析[J]. , 2013, 40(10): 184-188.

GUO Zhen-gang, ZHANG Li-chun, CAO Yang, YU Yong-sheng, LUO Xiao-tong, JIN Hai-guo. Correlations Analysis between Polymorphism of 3’-UTR in MSTN Gene and Slaughter Traits of China Red Steppe[J]. , 2013, 40(10): 184-188.

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