卵泡抑素基因过表达对其通路上相关基因表达的影响

›› 2013, Vol. ›› Issue (5): 35-39.

卵泡抑素基因过表达对其通路上相关基因表达的影响

孙亚蒙¹, 张冬杰², 张旭¹, 汪亮², 解宇¹, 刘娣^1,2

1. 东北农业大学动物科学技术学院, 黑龙江哈尔滨 150030;
2. 黑龙江省农业科学院畜牧所, 黑龙江哈尔滨 150086

收稿日期:2012-10-12 出版日期:2013-05-20 发布日期:2013-05-27
通讯作者: 刘娣(1963-),女,黑龙江,教授,博士生导师,研究方向:动物遗传育种与繁殖。E-mail:liudi1963@163.com E-mail:liudi1963@163.com
作者简介:孙亚蒙(1986-),男,山东人,博士生,研究方向:动物遗传育种与繁殖。
基金资助:
转基因生物新品种培育重大专项(2011ZX08006-003)。

Effect of FST Gene Over-expression on Expression of Related Genes in its Pathway

SUN Ya-meng¹, ZHANG Dong-jie², ZHANG Xu¹, WANG Liang², XIE Yu¹, LIU Di^1,2

1. College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China;
2. Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

Received:2012-10-12 Online:2013-05-20 Published:2013-05-27

摘要/Abstract

摘要： 为了探讨过表达卵泡抑素(follistatin,FST)基因对其通路上相关基因表达量的影响,本研究采用PCR方法扩增了民猪的FST基因,构建真核表达载体pcDNA3.1(+)-Dsig-FST,对转染了重组质粒的成纤维细胞进行了鉴定,并采用实时荧光定量PCR检测通路上相关基因表达量的变化。结果表明,本研究成功克隆了民猪的FST基因,并在猪胎儿成纤维细胞中显著过表达,结果导致了ActRⅡB和BMP4基因显著下调 (P<0.05),Smad4和Myostatin基因有下调趋势,ActRⅡA基因有上调趋势。由此可见,FST基因的表达变化对其通路上部分相关基因的表达有不同程度的抑制或促进作用。

关键词: 卵泡抑素(FST); 克隆; 真核表达; 猪胎儿成纤维细胞; 通路基因

Abstract: To investigate the effect of follistatin (FST) gene over-expression on related genes in its pathway, this study cloned the FST gene of Min pig with PCR method, constrcuted eukaryotic expression vector pcDNA3.1(+)-Dsig-FST, identified fibroblast cells transfected with the recombinant plasmid, and detected the change of related genes in its pathway with Real-time PCR method. The results showed that FST gene of Min pig was successfully cloned and over-expressed significantly in the porcine fetal fibroblast cell, and then lead to ActRⅡB and BMP4 gene decline significantly (P<0.05),Smad4 and Myostatin gene had downward trend, ActRⅡA gene had the upward trend. In summary, FST gene changed in its expression had the different effect of degrees inhibition or promotion on its related genes.

Key words: follistatin (FST); clone; eukaryotic expression; porcine fetal fibroblast; pathway gene

中图分类号:

Q786

孙亚蒙, 张冬杰, 张旭, 汪亮, 解宇, 刘娣. 卵泡抑素基因过表达对其通路上相关基因表达的影响[J]. , 2013, (5): 35-39.

SUN Ya-meng, ZHANG Dong-jie, ZHANG Xu, WANG Liang, XIE Yu, LIU Di. Effect of FST Gene Over-expression on Expression of Related Genes in its Pathway[J]. China Animal Husbandry & Veterinary Medicine, 2013, (5): 35-39.

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