6-羟基多巴胺致帕金森病大鼠模型的建立与评价

›› 2012, Vol. 39 ›› Issue (12): 162-165.

6-羟基多巴胺致帕金森病大鼠模型的建立与评价

李泽鸿¹, 陶英楠^1,2, 刘继阳¹, 张帆¹

1. 吉林农业大学生命科学学院,吉林长春 130118;
2. 柳河县烟草公司,吉林柳河 130060

收稿日期:2012-06-04 出版日期:2012-12-20 发布日期:2012-12-19
作者简介:李泽鸿(1965-),女,吉林人,博士,副教授,硕士生导师,研究方向:生物药资源开发与抗癌药物。
基金资助:
吉林省教育厅"十一五"科学技术研究项目课题(吉教科和字2010第41号)。

The Establishment and Evaluation of Rats Model of the Parkinson’s Disease Cased by 6-OHDA

LI Ze-hong¹, TAO Ying-nan^1,2, LIU Ji-yang¹, ZHANG Fan¹

1. College of Life Science, Jilin Agricultural University, Changchun 130118,China;
2. Tobacco Company of Liuhe County, Liuhe 130060,China

Received:2012-06-04 Online:2012-12-20 Published:2012-12-19

摘要/Abstract

摘要： 应用6-羟基多巴胺(6-hydroxydopamine,6-OHDA)制造帕金森病(Parkinson’s disease,PD)大鼠模型,建模成功后采用组织化学方法检测模型黑质多巴胺能神经元变化,并从行为学及黑质多巴胺能神经元数目的变化对模型进行综合评价。取Wistar大鼠,采用立体定向法,进行右侧纹状体区6-OHDA 双点注射。结果表明,6-OHDA纹状体注射可成功的诱导帕金森大鼠模型。

关键词: 帕金森病; 6-羟基多巴胺; 多巴胺能神经元

Abstract: Appplicate the 6-hydroxydopanine(6-OHDA) to manufacture the rat’s model of Parkinson’s disease (PD), through the organizations of chemical method could dectect and evaluate the model comprehensivly from the changes of the behaviour and the number changes of dopaminergic neurons of nigra after the model succeed. Fetch Wistar rat by using stereotactic method, and then inject right striatum 6-OHDA into two-point. This study showed that 6-OHDA injection striatum could induce the rat model of Parkinson’s sucessfully.

Key words: Parkinson’s disease; 6-hydroxydopamine; dopaminergic neurons

中图分类号:

S865.1⁺2

李泽鸿, 陶英楠, 刘继阳, 张帆. 6-羟基多巴胺致帕金森病大鼠模型的建立与评价[J]. , 2012, 39(12): 162-165.

LI Ze-hong, TAO Ying-nan, LIU Ji-yang, ZHANG Fan. The Establishment and Evaluation of Rats Model of the Parkinson’s Disease Cased by 6-OHDA[J]. , 2012, 39(12): 162-165.

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