精原干细胞介导法制备转基因羊的研究进展

›› 2012, Vol. 39 ›› Issue (12): 144-149.

精原干细胞介导法制备转基因羊的研究进展

邱峰龙, 李碧春

扬州大学动物科学与技术学院,江苏省动物繁育与分子设计重点实验室,江苏扬州 225009

收稿日期:2012-06-26 出版日期:2012-12-20 发布日期:2012-12-19
通讯作者: 李碧春(1963-),女,陕西人,博士生导师,教授,研究方向:胚胎工程。E-mail:yubcli@yzu.edu.cn;Tel:0514-87997207 E-mail:yubcli@yzu.edu.cn;Tel:0514-87997207
作者简介:邱峰龙(1989-),男,山东人,硕士,研究方向:胚胎工程。
基金资助:
转基因生物新品种培育重大专项(2011ZX08008-003);国家高技术研究发展计划(2011AA100307-04);江苏高校优势学科建设工程资助项目。

Research Progress on Transgenic Sheep Produced by Spermatogonial Stem Cell-Mediated

QIU Feng-long, LI Bi-chun

Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2012-06-26 Online:2012-12-20 Published:2012-12-19

摘要/Abstract

摘要： 精原干细胞介导法制备转基因动物是用试验导入的方法将外源基因移入动物细胞并整合到其基因组中,伴随着精原干细胞分化成精子,通过受精最终使外源基因得以表达。近年来,随着对精原干细胞研究的不断深入,人们发现其在建立转基因动物方面具有巨大的应用潜力和优势。作者从精原干细胞的生物学特性及携带外源基因的原理等方面阐述了其应用于转基因动物制备的理论机制,同时介绍了精原干细胞介导法在制备转基因动物方面,特别是转基因羊生产中的应用现状。

关键词: 精原干细胞介导法; 外源基因; 转基因羊

Abstract: Spermatogonial stem cell-mediated is to transfer the exogenous genes into the animal spermatogonia and the exogenous genes was integrated into its genome. With the original spermatogonial stem cells (SSCs) differentiation into sperm, the exogenous genes were expressed in their own offspring through fertilization. In recent years, people have realized its huge applications prospect and advantages in producing transgenic animals with the deepen research of SSCs. Then, the biological characteristics of SSCs as well as the principle of carrying exogenous gene into animal genome was described to clarify its theoretical mechanisms in preparation of transgenic animals, meanwhile, the research status of spermatogonial stem cell-mediated was introduced, especially for its application in the transgenic goat or sheep production.

Key words: spermatogonial stem cell-mediated; exogenous gene; transgenic sheep

中图分类号:

S813.3

邱峰龙, 李碧春. 精原干细胞介导法制备转基因羊的研究进展[J]. , 2012, 39(12): 144-149.

QIU Feng-long, LI Bi-chun. Research Progress on Transgenic Sheep Produced by Spermatogonial Stem Cell-Mediated[J]. , 2012, 39(12): 144-149.

参考文献

1 丁晓麟,张寒莹,石国庆,等.利用精原干细胞法生产转基因动物的研究进展[J].生命科学研究,2007,11(4):1~5.
2 马良宏,丁强,冯立新,等.精原干细胞移植后的体内迁移、增殖和分化[J].中国组织工程研究与临床康复,2010,14(32):5976~5982.
3 孙可,朱宝长.精原干细胞的增殖分化及其在畜牧业上的应用[J].安徽农业科学,2009,37(21):10004~10007.
4 孙思宇,李碧春,魏彩霞,等.鸡精原干细胞体外培养[J].中国兽医学报,2008,28(1):102~105.
5 孙大林,张新东.精原干细胞增殖和分化相关因子的研究进展[J].中华男科学杂志,2010,1(3):268~272.
6 孙敏,倪黎刚,施青青,等.睾丸注射Mx基因生产抗病转基因鸡初探[J].扬州大学学报(农业与生命科学版),2010,31(2):14~18.
7 刘世学,唐伟,熊涛.人精原干细胞分离、培养及鉴定[J].第三军医大学学报,2011,33(6):570~573.
8 刘鹏,敖红,潘杰. 精原干细胞的分选、培养及转基因应用探讨[J].中国畜牧兽医, 2009, 36(10): 84~87.
9 阴彦辉,孙敏,陈庭锋,等.睾丸注射法制备携带山羊H-FABP基因的转基因小鼠[J].遗传,2012,34(6):37~42.
10 李碧春,周冠月,吴洪,等.鸡睾丸细胞体外分离培养初探[J].扬州大学学报(农业与生命科学版),2007,28(1):21~25.
11 李泽兵,王彦军.世界首例绵羊精原干细胞移植实验获得成功[J].四川畜牧兽医,2009,8:57.
12 李福兵,魏泓,孙新民,等.睾丸内注射pEGFP-N1体内转染精子并在山羊早期胚胎成功表达[J].中国实验动物学报,2005,13(2):110~113.
13 张学明,李德雪,于家傲,等.精原干细胞的生物学特性[J].细胞生物学杂志,2006(1):37~41.
14 沈新明,乔贵林,张玲,等.用曲细精管微注射法建立绿色荧光蛋白转基因小鼠[J].第一军医大学学报,2002,22(3):250~253.
15 陈晓宇,朱志伟,于福先,等.湖羊精原干细胞体外培养[J].浙江农业学报,2010,22(5):580~584.
16 采克俊,张易祥,丁志丽,等.精原干细胞的分离纯化方法[J].上海畜牧兽医,2008(1):67~69.
17 赵君,刘彬,任文陟,等.体内精原干细胞转染法建立转基因小鼠[J].实验生物学报,2003,36(3):197~201.
18 贾映辉,金梅,刘鹏,等.家畜精原干细胞移植研究进展[J].安徽农业科学,2009,37(3):1108~1110.
19 高华颖,曹阳,李世辉,等.精子介导转基因动物的制备[J].遗传,2003,25(3):283~290.
20 Aponte P M,Soda T,Derooij D G. Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor[J].Theriogenology,2006,65(9):1828~1847.
21 Baker M.Stem cells:Fast and furious[J].Nature,2009,458(7241):962~965.
22 Barroca V,Lassalle B,Coureuil M,et al.Mouse differentiating spermatogonia can generate germinal stem cells in vivo[J].Nat Cell Biol,2009,11(2):190~196.
23 Brinster R L,Zimmermann J W. Spermatogenesis following male germcell transplanttation[J].Proc Natl Acad Sci, 1994, 91(1): 298~302.
24 Brinster R L,Ararbock M R.Germline transmission of donor hap lotype following spermatogonial transplantation[J]. Proc Natl Acad Sci USA, 1994,91(24):11303~11307.
25 Derooij D G,Grootegoed J A.Spermatogonial stem cells[J].Curr Opin Cell Biol,1998,10:694~701.
26 Ehmcke J,Hubner K,Scholer H R,et al.Spermatogonia:Origin,physiology and prospects for conservation and manipulation of the male germ line[J].Reprod Fertil Dev,2006,18:72~12.
27 Farre L,Rigau T,Mogas T,et al.Adenovirus-mediated introduction of DNA into pig sperm and offspring[J].Mol Reprod Der,1999,53(2):149~158.
28 Hammer R E,Pursel V G,Rexroad C E, et al.Production of transgenic rabbits,sheep and pigs by microinjection[J]. Nature, 1985, 315:680~683.
29 Hamra F K,Schultz N,Chapman K M,et al. Defining the spermatogonial stem cell[J]. Dev Biol,2004,269(2):393~410.
30 Hamra F K,Gatlin J,Chapman K M,et al. Production of transgenic rats by lentiviral transduction of male germline stem cells[J]. Proc Natl Acad Sci USA,2002, 99(23):14931~14936.
31 Hettle S J H,Harvey M J A,Cameron E R.Generation of transgenic sheep by sub-zonal injection of feline leukemia virus[J].Journal of Cellular Biochemistry Suppl, 1989, 13B:180.
32 Honaramooz A, Behboodi E, Megee S O, et al. Fertility and germline transmission of donor haplotype following germ cell transplantation in immunocompetent goats[J]. Biol Reprod,2003, 69(4): 1260~1264.
33 Honaramooz A, Behboodi E, Hausler C L, et al. Depletion of endogenous germ cells in male pigs and goats in preparation for germ cell transplantation[J]. J Androl,2005, 26(6):698~705.
34 Honaramooz A,Megee S,Zeng W X,et al.Adeno associated virus(AAV)mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation [J]. FASEB, 2008, 22(2): 374~382.
35 Kanatsu-Shinohara M,Toyokuni S,Shinohara T.Genetic selection of mouse male germline stem cell in vitro:Offspring from single stem cells[J].Biol Reprod,2005,72:236~240.
36 Kanatsu-Shinohara M,Ikawa M,Takehashi M,et al.Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells[J].Proc Natl Acad Sci USA,2006,103(21):8018~8023.
37 Kanatsu-Shinohara M,Kato M,Takehasshi M,et al.Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells[J].Biol Reprod,2008,79(6):1121~1128.
38 Lee A S,Tang C F,et al.Effects of cell number on teratoma formation by human embryonic stem cells[J].Cell Cycle,2009,8(16):2608~2612.
39 Li N,Lian Z X,Dai Y P.Production of the first transgenic goat expressing mAAT gene in ehlna[J].Biotechnology Information,2001,2:46~47.
40 Ogawa T,Dobrinski I,Brinster R L.Recipient preparation is critical for spermatogonial transplantation in the rat[J].Tissue Cell,1999,31(5):461~472.
41 Virginie O,Francois C.The spermatogonial stem cell:From basic knowledge to transgenic technology[J].Biochem Cell Biol,2005,(37):246~250.
42 Wu Z.Capacity for stochastic self-renewal and differentiation in mammalian spermatogonial stem cells[J].J Cell Biol,2009,187(4):513~524.

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